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Circulating miRNA 27a and miRNA150-5p; a noninvasive approach to endometrial carcinoma

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Abstract

The search for novel non-invasive biomarkers such as epigenetic molecular markers is new hope for common and burdensome cancers. We aim to assess serum expression of miRNA 27a and miRNA150-5p in endometrial cancer patients. Serum was drawn for 36 un-intervened endometrial cancer patients scheduled for hysterectomy and 35 controls. miRNA 27a and miRNA150-5p were measured by real time reverse transcription polymerase chain reaction. Significant overexpression of both miRNA in patients (p < 0.001). At cutoffs 0.2872 & > 1.02, miRNA 27a showed 100% sensitivity, specificity, positive and negative predictive values. miRNA150-5p showed 88.89% sensitivity, 100% specificity, 100% positive and 78.9% negative predictive values. Areas under curve were 1.0 for miRNA 27a, 0.982 for miRNA 150 performing much better than Ca125. miRNA 27a was significantly associated with type I endometroid endometrial cancer. Conclusion: miRNA 27a and miRNA-150-5P can be suggested as promising biomarkers of endometrial cancer possibly part of a miRNA panel for management.

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Authors and Affiliations

  1. Medical Biochemistry Department, Faculty of Medicine, Alexandria University, Champollion street, Al Mesallah Sharq, Al Attarin, Alexandria, Egypt

    Rasha Abdelmawla Ghazala

  2. Chemical Pathology Department, Medical Research Institute, Alexandria University, 165 El-Horreya Avenue, El- Hadra, Alexandria, 21561, Egypt

    Eman Ahmed El-Attar

  3. Department of Obstetrics and Gynecology, Faculty of Medicine, Alexandria University, Champollion street, Al Mesallah Sharq, Al Attarin, Alexandria, Egypt

    Ziad Sami Abouzeid

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  1. Rasha Abdelmawla Ghazala
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All authors whose names appear on the submission (1) made substantial contributions to the conception or design of the work; or the acquisition, analysis, or interpretation of data; or the creation of new software used in the work; (2) drafted the work or revised it critically for important intellectual content; (3) approved the version to be published; and (4) agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

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The Research Ethics Committee of the Faculty of Medicine, University of Alexandria IRB No 0012098, FWA No 00018699 approved this study and was conducted in accordance with the provisions of the Declaration of Helsinki and Good Clinical Practice guidelines.

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Ghazala, R.A., El-Attar, E.A. & Abouzeid, Z.S. Circulating miRNA 27a and miRNA150-5p; a noninvasive approach to endometrial carcinoma. Mol Biol Rep 48, 4351–4360 (2021). https://doi.org/10.1007/s11033-021-06450-6

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