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Expression of transient receptor potential vanilloid genes and proteins in diabetic rat heart

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Abstract

Cardiac complications are leading causes of death in diabetic patients. Imbalance of Ca2+ homeostasis is a hallmark of cardiac dysfunction in diabetes, while TRPV channels are non-selective for cations and are permeable to Ca2+. Our aim was to evaluate the expression levels of TRPV1, TRPV2, TRPV3, TRPV4, TRPV5, and TRPV6 genes and proteins in cardiac tissue at 3 days and 4, 8, and 12 weeks after induction of diabetes. Sprague-Dawley rats were assigned to control and DM groups. DM was induced by intraperitoneal injection of streptozotocin (60 mg/kg). The expression levels of TRPV genes were analyzed by the quantitative reverse transcription polymerase chain reaction, and TRPV proteins were determined by western blotting. Compared to controls, the expression levels of TRPV2, TRPV3, and TRPV6 in diabetic myocardium did not change, while TRPV1 decreased at 4, 8, and 12 weeks, TRPV4 was upregulated at 3 days and 4, 8, and 12 weeks, TRPV5 mRNA increased at 8 and 12 weeks, and TRPV5 protein increased at 4, 8, and 12 weeks. Our findings showed that TRPV1, TRPV4, and TRPV5 are associated with the diabetic heart.

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Abbreviations

DM:

Diabetes mellitus

qRT-PCR:

Quantitative reverse transcription polymerase chain reaction       

RT:

Reverse transcription

PCR:

Polymerase chain reaction

SD:

Standard deviation

STZ:

Streptozotocin

BCA:

Bicinchoninic acid

TRP:

Transient receptor potential

TRPV:

Transient receptor potential vanilloid

ATP:

5'-Adenylate triphosphate

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

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Acknowledgements

This study was supported by grants from the National Natural Science Foundation of China (30872716), the Natural Science Foundation of Hubei Province (2015CFB288), and a Health and Family Planning Project in Hubei Province (WJ2015MB171).

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  1. Xiaoli Jia and Tao Yu have contributed equally to this work.

Authors and Affiliations

  1. Department of Physiology, Medical Science College of China Three Gorges University, Yichang, China

    Xiaoli Jia, Chao Xiao, Deqiao Sheng, Mengcheng Yang, Quanyi Cheng, Jing Wu, Ting Lian, Yun Zhao & Shizhong Zhang

  2. Renhe Hospital of China Three Gorges University, Yichang, China

    Tao Yu

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  1. Xiaoli Jia
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Contributions

X.L.J. and T.Y. carried out the experiments and drafted the manuscript. C.X. and D.Q.S. was involved in data analysis. M.C.Y., Q.Y.C., J.W., and T.L. contributed to conducting the experiments. Y.Z. and S.Z.Z. reviewed the data, and revised the manuscript.

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Correspondence to Yun Zhao or Shizhong Zhang.

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Jia, X., Yu, T., Xiao, C. et al. Expression of transient receptor potential vanilloid genes and proteins in diabetic rat heart. Mol Biol Rep 48, 1217–1223 (2021). https://doi.org/10.1007/s11033-021-06182-7

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