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Transcriptional and epigenetic effects of Vitis vinifera L. leaf extract on UV-stressed human dermal fibroblasts

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A Correction to this article was published on 15 June 2023

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Abstract

Adverse environmental conditions such as UV radiation induce oxidative and aging events leading to severe damage to human skin cells. Natural products such as plant extracts have been implicated in the skin anti-oxidant and anti-aging cellular protection against environmental stress. Moreover, environmental factors have been shown to impact chromatin structure leading to altered gene expression programs with profound changes in cellular functions. In this study, we assessed the in vitro effect of a leaf extract from Vitis vinifera L. on UV-stressed primary human dermal fibroblasts, focusing on gene expression and DNA methylation as an epigenetic factor. Expression analysis of two genes known to be implicated in skin anti-aging, SIRT1and HSP4, demonstrated significant induction in the presence of the extract under normal or UVA conditions. In addition, DNA methylation profiling of SIRT1 and HSP47 promoters showed that the V. vinifera L. extract induced changes in the DNA methylation pattern of both genes that may be associated with SIRT1 and HSP47 gene expression. Our study shows for the first time transcriptional and DNA methylation alterations on human skin fibroblasts exposed to UV stress and suggest a protective effect of a V. vinifera extract possibly through transcriptional regulation of critical skin anti-aging genes.

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Authors and Affiliations

  1. Laboratory of Biochemistry, Department of Research and Development, APIVITA S.A., Industrial Park of Markopoulo Mesogaias, 19003, Markopoulo Attiki, Athens, Greece

    Sophia Letsiou & Aliki Kapazoglou

  2. Department of Vitis, Institute of Olive Tree, Subtropical Crops and Viticulture (IOSV), Hellenic Agricultural Organization-Demeter (HAO-Demeter), Lykovrysi, 14123, Athens, Greece

    Aliki Kapazoglou

  3. Department of Genetics and Plant Breeding, Aristotle University of Thessaloniki, University Campus, 54124, Thessaloniki, Greece

    Athanasios Tsaftaris

  4. Perrotis College, American Farm School, Thermi, 570 01, Thessaloniki, Greece

    Athanasios Tsaftaris

  5. Raw Materials & Extraction Specialist, Department of Research and Development, APIVITA SA, 19003, Markopoulo Attiki, Athens, Greece

    Eleni Spanidi

  6. Research and Development Director, Department of Research and Development, APIVITA S.A., 19003, Markopoulo Attiki, Athens, Greece

    Konstantinos Gardikis

Authors
  1. Sophia Letsiou
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  2. Aliki Kapazoglou
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  4. Eleni Spanidi
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  5. Konstantinos Gardikis
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Contributions

SL conceived, planned and oversaw the experiments. SL carried out the experiments on the RT-qPCR analysis, AK carried out experiments on epigenetics analysis. SL and AK analyzed and integrated the datasets and drafted the manuscript. AT critically read and contributed to improve the MS. K.G. made extract preparations K.G. critically read and contributed to improve the MS. E.S. made extract preparations and optimization and collected the plant material.

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Correspondence to Sophia Letsiou.

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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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The original online version of this article was revised: The authors "Eleni Spanidi" and "Konstantinos Gardikis" are included as 4th and 5th authors. The sections "Materials and methods, Discussions, Author contributions and funding" are updated.

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Letsiou, S., Kapazoglou, A., Tsaftaris, A. et al. Transcriptional and epigenetic effects of Vitis vinifera L. leaf extract on UV-stressed human dermal fibroblasts. Mol Biol Rep 47, 5763–5772 (2020). https://doi.org/10.1007/s11033-020-05645-7

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  • DOI: https://doi.org/10.1007/s11033-020-05645-7

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