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RETRACTED ARTICLE: Knockdown of filaggrin influences the epidermal terminal differentiation via MAPK pathway in normal human epidermal keratinocytes

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This article was retracted on 18 August 2015

Abstract

We aimed to gain further insight into the role of the MAPK signaling pathway in terminal differentiation of normal human epidermal keratinocytes (NHEKs) with filaggrin knockdown. Filaggrin expression was knocked down by shRNA technology and the MAPK pathways were inhibited by three different inhibitors in NHEKs. The associated mRNAs and proteins were investigated by RT-PCR and western blot. Filaggrin absence inhibited the expression of differentiation-associated proteins, and blocked the protein expression of p38 MAPK, ERK1/2, JNK, Akt and NF-κB. Moreover, inhibited p38 MAPK, instead of ERK1/2 or JNK, lead to decreases in the expressions of Akt, NF-κB, and differentiation- associated proteins. In conclusion, Filaggrin might affect the epidermal terminal differentiation mainly through the p38-MAPK, NF-κB and Akt pathways. ERK1/2 and JNK might also be involved in the process.

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Acknowledgments

The study was supported by National Natural Science Foundation of China (NSFC) (No. 81101183, 81170771, 81272588) and China Postdoctoral Science Foundation (CPSF) (No. 2014M550370).

Conflict of interest

All authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Dermatology, Jinan Central Hospital Affiliated to Shandong University, No. 105 Jiefang Road, Jinan, 250013, Shandong Province, China

    Ningning Dang, Haiyan Song & Xiaoran Zhang

  2. Department of Endocrinology, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013, Shandong Province, China

    Shuguang Pang

  3. Department of Neurology, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013, Shandong Province, China

    Hong Bian

  4. College of Life Science, Shandong Normal University, Jinan, 250014, Shandong Province, China

    Ningning Dang & Liguo An

  5. Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, No. 105 Jiefang Road, Jinan, 250013, Shandong Province, China

    Xiaoli Ma

Authors
  1. Ningning Dang
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  2. Shuguang Pang
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  3. Haiyan Song
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  4. Hong Bian
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  5. Xiaoran Zhang
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  6. Liguo An
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  7. Xiaoli Ma
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Corresponding authors

Correspondence to Ningning Dang or Xiaoli Ma.

Additional information

Ningning Dang and Shuguang Pang contributed equally to this work. Ningning Dang is the co-correspondence author.

The Publisher and Editor retract this article in accordance with the recommendations of the Committee on Publication Ethics (COPE). After a thorough investigation we have strong reason to believe that the peer review process was compromised.

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Dang, N., Pang, S., Song, H. et al. RETRACTED ARTICLE: Knockdown of filaggrin influences the epidermal terminal differentiation via MAPK pathway in normal human epidermal keratinocytes. Mol Biol Rep 42, 337–343 (2015). https://doi.org/10.1007/s11033-014-3765-6

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  • DOI: https://doi.org/10.1007/s11033-014-3765-6

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