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Polymorphism of insulin-like growth factor 1 gene and its association with litter size in Small Tail Han sheep

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Abstract

The insulin-like growth factor 1 (IGF1) gene was studied as a candidate gene for high prolificacy in sheep. Polymorphisms of 5′ regulatory region and all four exons of IGF1 gene were detected in Small Tail Han (n = 277), Hu (n = 58), Texel (n = 48) and Dorset (n = 46) sheep by PCR-RFLP and PCR-SSCP analysis. A microsatellite polymorphic site and a restriction fragment length polymorphism were shown in the 5′ regulatory region of IGF1 gene. The ewes with genotype 123/123 bp had 0.81 (P < 0.05) or 1.03 (P < 0.01) lambs more than those with genotype 125/125 bp or 125/127 bp, the ewes with genotype 123/125 bp had 0.46 (P < 0.05) or 0.68 (P < 0.01) lambs more than those with genotype 125/125 bp or 125/127 bp. In addition, there were two mutations (C1511G and A1513G) in 5′ regulatory region of IGF1 gene. The ewes with genotype BB or AB had 0.96 (P < 0.05) or 0.38 (P < 0.05) lambs more than those with genotype AA, but there were no significant differences between BB and AB genotypes (P > 0.05) in Small Tail Han sheep. These results preliminarily indicated that these polymorphisms of IGF1 gene could be used in molecular marker-assisted selection for sheep breeding programs.

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Acknowledgments

This work was supported by National Key Technology Research and Development (R&D) Program of China (No. 2008BADB2B01), by the earmarked fund for China Agriculture Research System (No. CARS-39), by National Key Basic R&D Program of China (No. 2006CB102105), and by Special Fund for Basic Scientific Research of Institute of Animal Science, Chinese Academy of Agricultural Sciences (No. 2010jc-9).

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Authors and Affiliations

  1. Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China

    J. N. He, M. X. Chu, T. Feng, R. Di, L. Fang, D. W. Huang & Q. Q. Tang

  2. College of Bioengineering, Chongqing University, Chongqing, 400030, People’s Republic of China

    B. Y. Zhang & P. Q. Wang

  3. College of Agriculture, Liaocheng University, Liaocheng, 252059, People’s Republic of China

    G. L. Cao

  4. State Key Laboratory of Agricultural Biotechnology, China Agricultural University, Beijing, 100193, People’s Republic of China

    N. Li

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  1. J. N. He
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Correspondence to M. X. Chu.

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He, J.N., Zhang, B.Y., Chu, M.X. et al. Polymorphism of insulin-like growth factor 1 gene and its association with litter size in Small Tail Han sheep. Mol Biol Rep 39, 9801–9807 (2012). https://doi.org/10.1007/s11033-012-1846-y

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  • DOI: https://doi.org/10.1007/s11033-012-1846-y

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