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Nearly complete mitochondrial genome of Polyascus gregaria and the phylogenetic relationships among maxillopodans

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Abstract

We determined for the first time the nearly complete mitochondrial genome sequence of the entozoic Polyascus gregaria, a representative of Rhizocephala, Cirripedia. The nearly complete mitogenome was 15, 465 bp in length, consisting of 11 protein-coding genes, two rRNA genes, 22 tRNA genes and one major incomplete noncoding region. In total there are 73 overlapping nucleotides and 17 spacers between genes. All genes sequenced in P. gregaria mtDNA (including RNAs) were encoded on the same strand of the DNA, and the gene arrangement differed from that of other metazoan animals. The mitochondrial genome rearrangements included translocation of at least 8 genes and even inversion of the coding polarity of at least 2 genes. Comparative analysis of the gene orders with other maxillopodan mtDNAs showed that the unique characteristics of the thoracican cirripeds lineage were not observed in this representative of rhizocephalan. Phylogenetic analyses supported a close affinity of Rhizocephala to Thoracica. By adding the mitochondrial genomes from 4 copepods, the reciprocally monophyletic cirripeds and copepods clustered as sister groups, refusing the close relationship between Cirripedia and Remipedia. However, the monophyly of Maxillopoda was not supported in this study.

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Acknowledgments

The authors are indebted to Dongxi Shen for providing the material. We especially thank the reviewers for their critical reading, insightful comments and suggestions on this manuscript. This work was supported by grants from the Priority Academic Program Development of Jiangsu Higher Education Institutions, and Nanjing Normal University Innovative Team Project (No. 0319PM0902).

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  1. Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210046, China

    Jie Yan, Jianli Zhou, Peng Li, Hongying Sun & Kaiya Zhou

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  1. Jie Yan
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  2. Jianli Zhou
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  3. Peng Li
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  4. Hongying Sun
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  5. Kaiya Zhou
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Correspondence to Kaiya Zhou.

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Yan, J., Zhou, J., Li, P. et al. Nearly complete mitochondrial genome of Polyascus gregaria and the phylogenetic relationships among maxillopodans. Mol Biol Rep 39, 7413–7419 (2012). https://doi.org/10.1007/s11033-012-1573-4

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  • DOI: https://doi.org/10.1007/s11033-012-1573-4

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