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Characterization of the expression profile of calpain-3 (CAPN3) gene in chicken

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Abstract

Calpain-3 is a skeletal muscle-specific protease and participates in the regulation of myogenesis. In this study, we quantified the expression of calpain-3 (CAPN3) mRNA in a Chinese local chicken breed (Sichuan Mountainous Black-boned chicken [MB]), to discern the tissue and ontogenic expression pattern. Meanwhile, we compared the CAPN3 mRNA expression pattern in MB chicken at 10 weeks with a commercial meat type chicken line (S01) of the same age to identify the unique expression pattern under different genetic background. A real time quantitative PCR (qRT-PCR) assay was developed for an accurate measurement of its expression in various tissues from chickens at different ages (0, 2, 4, 6, 8, 10, and 12 weeks). Expression of the CAPN3 mRNA was detected in the selected tissues, regardless of age. The breast muscle and leg muscle tissues had a significantly higher expression than the other tissues from the same individual (P < 0.01). Overall, the CAPN3 mRNA level exhibited a “rise-decline” developmental change in detected tissues except for brain. The S01 chicken had a higher expression of the CAPN3 mRNA in detected tissues than the MB chicken at 10 weeks. The present expression data of chicken CAPN3 gene may provide some information to shed light on the tissue and ontogenic expression pattern during chicken development.

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References

  1. Goll DE, Thompson VF, Li H, Wei WEI, Cong J (2003) The calpain system. Physiol Rev 83(3):731–801

    PubMed  CAS  Google Scholar 

  2. Geesink GH, Ilian MA, Morton JD, Bickerstaffe R (2000) Involvement of calpains in postmortem tenderisation: a review of recent research. Proc N Z Soc Anim Prod 60:99–102

    Google Scholar 

  3. Hopkins DL, Thompson JM (2002) Factors contributing to proteolysis and disruption of myofibrillar proteins and the impact on tenderisation in beef and sheep meat. Aust J Agric Res 53(2):149–166

    Article  CAS  Google Scholar 

  4. Koohmaraie M, Kent MP, Shackelford SD, Veiseth E, Wheeler TL (2002) Meat tenderness and muscle growth: is there any relationship? Meat Sci 62(3):345–352

    Article  PubMed  Google Scholar 

  5. Kinbara K, Ishiura S, Tomioka S, Sorimachi H, Jeong SY, Amano S, Kawasaki H, Kolmerer B, Kimura S, Labeit S (1998) Purification of native p94, a muscle-specific calpain, and characterization of its autolysis. Biochem J 335(Pt 3):589–596

    PubMed  CAS  Google Scholar 

  6. Taylor RG, Geesink GH, Thompson VF, Koohmaraie M, Goll DE (1995) Is Z-disk degradation responsible for postmortem meat tenderization? J Anim Sci 72:1351–1367

    Google Scholar 

  7. Sorimachi H, Imajoh-Ohmi S, Emori Y, Kawasaki H, Ohno S, Minami Y, Suzuki K (1989) Molecular cloning of a novel mammalian calcium-dependent protease distinct from both m-and mu-types. Specific expression of the mRNA in skeletal muscle. J Biol Chem 264(33):20106–20111

    PubMed  CAS  Google Scholar 

  8. Sorimachi H, Ohmi S, Emori Y, Kawasaki H, Saido TC, Ohno S, Minami Y, Suzuki K (1990) A novel member of the calcium-dependent cysteine protease family. Biol Chem Hoppe Seyler 371:171–176

    PubMed  CAS  Google Scholar 

  9. Sorimachi H, Tsukahara T, Okada-Ban M, Sugita H, Ishiura S, Suzuki K (1995) Identification of a third ubiquitous calpain species—chicken muscle expresses four distinct calpains. Biochim Biophys Acta 1261(3):381–393

    PubMed  Google Scholar 

  10. Parr T, Sensky PL, Scothern GP, Bardsley RG, Buttery PJ, Wood JD, Warkup C (1999) Relationship between skeletal muscle-specific calpain and tenderness of conditioned porcine longissimus muscle. J Anim Sci 77(3):661–668

    PubMed  CAS  Google Scholar 

  11. Ilian MA, Bekhit AED, Bickerstaffe R (2004) The relationship between meat tenderization, myofibril fragmentation and autolysis of calpain 3 during post-mortem aging. Meat Sci 66(2):387–397

    Article  PubMed  Google Scholar 

  12. Ilian MA, Bekhit AEDA, Stevenson B, Morton JD, Isherwood P, Bickerstaffe R (2004) Up-and down-regulation of longissimus tenderness parallels changes in the myofibril-bound calpain 3 protein. Meat Sci 67(3):433–445

    Article  PubMed  CAS  Google Scholar 

  13. Ilian MA, Morton JD, Bekhit AED, Roberts N, Palmer B, Sorimachi H, Bickerstaffe R (2001) Effect of preslaughter feed withdrawal period on longissimus tenderness and the expression of calpains in the ovine. J Agric Food Chem 49(4):1990–1998

    Article  PubMed  CAS  Google Scholar 

  14. Ilian MA, Morton JD, Kent MP, Le Couteur CE, Hickford J, Cowley R, Bickerstaffe R (2001) Intermuscular variation in tenderness: association with the ubiquitous and muscle-specific calpains. J Anim Sci 79(1):122

    PubMed  CAS  Google Scholar 

  15. Geesink GH, Taylor RG, Koohmaraie M (2005) Calpain 3/p94 is not involved in postmortem proteolysis. J Anim Sci 83(7):1646–1652

    PubMed  CAS  Google Scholar 

  16. Ono Y, Kakinuma K, Torii F, Irie A, Nakagawa K, Labeit S, Abe K, Suzuki K, Sorimachi H (2004) Possible regulation of the conventional calpain system by skeletal muscle-specific calpain, p94/calpain 3. J Biol Chem 279(4):2761–2771

    Article  PubMed  CAS  Google Scholar 

  17. Jones SW, Parr T, Sensky PL, Scothern GP, Bardsley RG, Buttery PJ (1999) Fibre type-specific expression of p94, a skeletal muscle-specific calpain. J Muscle Res Cell Motility 20(4):417–424

    Article  CAS  Google Scholar 

  18. Van den Hemel-Grooten HN, Te Pas MF, Van den Bosch TJ, Garssen GJ, Schreurs VV, Verstegen MW (1997) mRNA levels of the calpain system in longissimus muscle of young pigs during prolonged feeding of a protein-free diet. J Anim Sci 75(4):968–974

    PubMed  Google Scholar 

  19. Yang X, Chen J, Xu Q, Zhao R (2007) Muscle type-specific responses of myoD and calpain 3 expression to recombinant porcine growth hormone in the pig. Animal 1(07):989–996

    Article  CAS  Google Scholar 

  20. Dargelos E, Moyen C, Dedieu S, Veschambre P, Poussard S, Vuillier-Devillers K, Brustis JJ, Cottin P (2002) Development of an inducible system to assess p94 (CAPN3) function in cultured muscle cells. J Biotechnol 96(3):271–279

    Article  PubMed  CAS  Google Scholar 

  21. Lee HL, Sante-Lhoutellier V, Vigouroux S, Briand Y, Briand M (2007) Calpain specificity and expression in chicken tissues. Comp Biochem Physiol B Biochem Mol Biol 146(1):88–93

    Article  PubMed  Google Scholar 

  22. Zhang ZR, Liu YP, Yao YG, Jiang XS, Du HR, Zhu Q (2009) Identification and association of the single nucleotide polymorphisms in calpain 3(CAPN 3) gene with carcass traits in chickens. BMC Genet 10(1):10

    Article  PubMed  CAS  Google Scholar 

  23. Zhao XL, Yang ZQ, Shu G, Zhu Q (2007) Testing the meat traits of Sichuan Shandi Black-bone chicken and having correlation analysis for its carcass traits and meat traits. The animal husbandry and veterinary association of Sichuan province in 2007 conference

  24. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-[Delta][Delta] CT method. Methods 25(4):402–408

    Article  PubMed  CAS  Google Scholar 

  25. Pearson AM, Young RB (1989) Muscle and meat biochemistry. In Bernard SS, Steve LT (eds) Food science and technology. Academic Press, Inc, pp 8–29

  26. Swatland HJ (1994) Structure and Development of Meat Animals and Poultry. Technomic Publishing Co Inc Lancaster, PA

    Google Scholar 

  27. Lonergan SM, Deeb N, Fedler CA, Lamont SJ (2003) Breast meat quality and composition in unique chicken populations. Poul Sci 82(12):1990–1994

    CAS  Google Scholar 

  28. Medler S, Chang ES, Mykles DL (2007) Muscle-specific calpain is localized in regions near motor endplates in differentiating lobster claw muscles. Comp Biochem Physiol Part A Mol Integr Physiol 148(3):591–598

    Article  Google Scholar 

Download references

Acknowledgments

We thank the four anonymous reviewers for their helpful comments on the early version of the manuscript and Ling Yang for technical support. This study was supported by the Program for New Century Excellent Talents in University (NCET-10-0889), the Science Fund for Young Scholars in Sichuan Province (ZQ 026-017), the foundation program of Sichuan Animal Science Academy (SASA2010A02), the Ministry of Agriculture of China (No. 2009ZX08009-159B) and Yunnan Province (2009CI119).

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Authors and Affiliations

  1. College of Animal Science and Technology, Sichuan Agricultural University, 46# Xinkang Road, Ya’an, Sichuan, 625014, China

    Zeng-Rong Zhang, Qing Zhu & Yi-Ping Liu

  2. Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, 650223, China

    Yong-Gang Yao

  3. Sichuan Animal Science Academy, Chengdu, Sichuan, 610066, China

    Zeng-Rong Zhang & Xiao-Song Jiang

  4. Sichuan Dahen Poultry Breeding Company, Chengdu, Sichuan, 610066, China

    Hua-Rui Du

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  1. Zeng-Rong Zhang
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  2. Qing Zhu
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Correspondence to Yi-Ping Liu.

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Zhang, ZR., Zhu, Q., Yao, YG. et al. Characterization of the expression profile of calpain-3 (CAPN3) gene in chicken. Mol Biol Rep 39, 3517–3521 (2012). https://doi.org/10.1007/s11033-011-1124-4

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  • DOI: https://doi.org/10.1007/s11033-011-1124-4

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