Abstract
Glutathione S-transferases (GSTs) are a multifunctional super gene family, some of which play an important role in insecticide resistance. In this research, we used a real-time quantitative RT-PCR method, and a novel strategy, to measure the transcriptional level per gene copy using an exogenous RNA reference and DNA reference. The transcription levels of six BmGST genes in different tissues of fifth instar Bombyx mori larvae and their responses to insecticide and fluoride were investigated. The results show different levels and patterns of expression of the different BmGSTs in the various tissues observed. The BmGSTs can be induced by insecticide and fluoride, but their responses to each are different. The results of this research are helpful in studying the tissue-specific expression of BmGSTs in Bombyx mori, and in developing new pesticide resistant silkworm varieties.
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This work was supported by the foundation of post scientist in National Sericultural System (nycytx-27-gw307) and the project 31072086 supported by National Natural Science Foundation of China.
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Guo-dong Zhao and Yi-ling Zhang contributed equally to this work.
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Zhao, Gd., Zhang, Yl., Gao, Rn. et al. Quantitative analysis of expression of six BmGST genes in silkworm, Bombyx mori . Mol Biol Rep 38, 4855–4861 (2011). https://doi.org/10.1007/s11033-010-0626-9
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DOI: https://doi.org/10.1007/s11033-010-0626-9