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A tagging SNP in ALOX5AP and risk of stroke: a haplotype-based analysis among eastern Chinese Han population

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Abstract

A genome-wide approach found significant association of two at-risk haplotypes (HapA, HapB) in the ALOX5AP gene with myocardial infarction and stroke. To date, it is still controversial whether ALOX5AP gene polymorphisms are risk factors for stroke. The aim of the present study is to investigate the association between the ALOX5AP gene polymorphism and the risk for stroke in Eastern Chinese Han population with a haplotype-based analysis. We conducted a comprehensive association study of 507 stroke patients and 510 healthy controls to assess the association between the ALOX5AP tagging single-nucleotide polymorphisms (tSNPs) and stroke risk. Genotyping was performed using the PCR–RFLP assay. In the single-locus analysis, we found that the rs9579646 AG genotype was associated with a marginally decreased risk for stroke (adjusted odds ratio, 0.65; 95% confidence interval, 0.45–0.96), compared with the AA genotype. Haplotype-based association analysis of block 2 involving rs10507391 and rs12429692 revealed that the decreased risk of stroke was significantly associated with haplotype AA (OR, 0.66; 95% CI, 0.46–0.95). These results suggested that the genetic variants in ALOX5AP might modulate the risk of stroke in Eastern Chinese Han population. The frequencies of single-marker alleles and haplotypes showed remarkable differences from those in other populations.

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Abbreviations

ALOX5AP/FLAP:

5-lipoxygenase activating protein

ALOX5:

5-lipoxygenase

BMI:

Body mass index

CAD:

Coronary artery disease

CI:

Confidence intervals

CT:

Computed tomography

CVD:

Cerebrovascular disease

DM:

Diabetes mellitus

HDL-C:

High density lipoprotein cholesterol

LD:

Linkage disequilibrium

MAF:

Minor allele frequency

MRI:

Magnetic resonance imaging

OR:

Odds ratios

PCR:

Polymerase chain reaction

RFLP:

Restriction fragment length polymorphism

SNPs:

Single-nucleotide polymorphisms

tSNPs:

Tagging single-nucleotide polymorphisms

TC:

Total cholesterol

TG:

Triglycerides

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Acknowledgments

This work was supported by Natural Science Foundation of Jiangsu Province (No. BS2006007) and Research Fundation of Jiangsu Province Hygiene Committee (No.H201005). We sincerely thank Chunye Luan, Xiaolei Gu and Shuang Lou for their assistance in recruiting the subjects; Cheng Chen, Juan Wu for their technical support; Guangfu Jin and Chenbo Ji for their laboratory assistance; Guixiang Ji and Xinliang Ding for assistance in data analysis.

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Authors and Affiliations

  1. Department of Emergency, First Affiliated Hospital, Nanjing Medical University, Guangzhou Road 300, 210029, Nanjing, China

    Hao Sun, Hao Wu, Jing Zhang & Jinsong Zhang

  2. Department of Emergency Medicine, Kangda College, Nanjing Medical University, Hanzhong Road 140, 210029, Nanjing, China

    Hao Wu

  3. Laboratory of Neurotoxicology, School of Public Health, Nanjing Medical University, Hanzhong Road 140, 210029, Nanjing, China

    Jun Wang, Haixia Ding & Hang Xiao

  4. Department of Epidemiology and Biostatistics, Nanjing Medical University, Hanzhong Road 140, 210029, Nanjing, China

    Ying Lu

Authors
  1. Hao Sun
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  2. Hao Wu
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  3. Jing Zhang
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  4. Jun Wang
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  5. Ying Lu
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  6. Haixia Ding
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  7. Hang Xiao
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  8. Jinsong Zhang
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Corresponding authors

Correspondence to Hang Xiao or Jinsong Zhang.

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Hao Sun and Hao Wu contributed equally.

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Sun, H., Wu, H., Zhang, J. et al. A tagging SNP in ALOX5AP and risk of stroke: a haplotype-based analysis among eastern Chinese Han population. Mol Biol Rep 38, 4731–4738 (2011). https://doi.org/10.1007/s11033-010-0610-4

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  • DOI: https://doi.org/10.1007/s11033-010-0610-4

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