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Apolipoprotein A1 −75 G/A and +83 C/T polymorphisms: susceptibility and prognostic implications in breast cancer

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Abstract

Apolipoprotein A1 (ApoA1) is the major apoprotein constituent of high-density lipoprotein that can play important roles in tumor invasion and metastasis. In the current report, we evaluated the role of the functional ApoA1 polymorphisms (−75 G/A and +83 C/T) as genetic markers for breast cancer susceptibility and prognosis. We used the polymerase chain reaction and restriction enzyme digestion (RFLP-PCR) to characterize the variations of the ApoA1 gene in 295 unrelated Tunisian patients with breast carcinoma and 197 healthy control subjects. No association was found between the +83 C/T genetic variation in ApoA1 gene and the risk of breast cancer occurrence. The presence of the (+83) T allele appeared however to be associated with an increased risk of lymph node metastasis occurrence (OR = 2.94; P = 0.01). Furthermore, a positive association was found between ApoA1 −75 A allele carriers and breast cancer risk (OR = 1.57; P = 0.02). Regarding prognostic indicators, a significant association was found between ApoA1 (−75) A allele carriers and the premenopausal status of breast cancer patients (OR = 1.73; P = 0.03). Additionally, the presence of the −75 A allele was correlated with the oestrogen receptor status among premenopausal women (OR = 2.45; P = 0.02). This is the first report on the studies of ApoA1 single nucleotide polymorphisms (SNPs) in breast carcinomas. Our data suggest that these genetic variations of ApoA1 may represent a marker for the increased risk of breast cancer.

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Abbreviations

BC:

Breast carcinoma

SNP:

Single nucleotide polymorphism

ER:

Oestrogen receptor

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Acknowledgments

This work was supported by le Ministère de l’Enseignement Supérieur et de la Recherche Scientifique, le Ministère de la Santé Publique de la République Tunisienne. We thank the staff of the Departments of Radiation Oncology and Medical Oncology of CHU F. Hached, Sousse, for providing samples and clinical information.

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Authors and Affiliations

  1. Faculté de Médecine de Monastir, Laboratoire d’Immuno-Oncologie Moléculaire, 5019, Monastir, Tunisia

    Bechr Hamrita, Hela Ben Nasr, Sallouha Gabbouj, Noureddine Bouaouina, Lotfi Chouchane & Karim Chahed

  2. Service de Cancérologie Radiothérapie CHU Farhat Hached, Sousse, Tunisia

    Noureddine Bouaouina

  3. Department of Genetic Medicine, Weill Cornell Medical College in Qatar, and Qatar Foundation, Doha, Qatar

    Lotfi Chouchane

  4. Faculty of Sciences, Sfax, Tunisia

    Karim Chahed

Authors
  1. Bechr Hamrita
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  2. Hela Ben Nasr
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  3. Sallouha Gabbouj
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  4. Noureddine Bouaouina
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  5. Lotfi Chouchane
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  6. Karim Chahed
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Correspondence to Karim Chahed.

Additional information

Bechr Hamrita and Hela Ben Nasr contributed equally to the study.

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Hamrita, B., Nasr, H.B., Gabbouj, S. et al. Apolipoprotein A1 −75 G/A and +83 C/T polymorphisms: susceptibility and prognostic implications in breast cancer. Mol Biol Rep 38, 1637–1643 (2011). https://doi.org/10.1007/s11033-010-0274-0

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  • DOI: https://doi.org/10.1007/s11033-010-0274-0

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