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Single nucleotide polymorphisms of the prolactin receptor (PRLR) gene and its association with growth traits in chinese cattle

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Abstract

Genetic polymorphism of the prolactin receptor (PRLR) gene was detected by PCR-SSCP and DNA sequencing methods in 665 individuals from five Chinese cattle breeds. The results showed that at the P1 locus, three observed genotypes (AA, AB and BB), two linked SNPs (G1267A and T1268C), and one missense mutation (S18N) within a putative signal peptide were determined. The frequencies of haplotypes A and B in the five breeds were 0.596–0.802 and 0.198–0.404, respectively. Polymorphism of the PRLR gene was shown to be significantly associated with growth traits in the Nanyang breed. Individuals with genotype BB had greater hucklebone width, body weight and average daily gain than those with genotype AA at 6 months old (P < 0.01), as well as better body height, body length and heart girth when 6 months (P < 0.05). This study revealed for the first time that the PRLR gene is a promising candidate gene that affects growth traits in cattle.

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References

  1. Bole-Feysot CH, Goffin V, Edery M, Binart N, Kelly PA (1998) Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice. Endocr Rev 19:225–268

    Article  CAS  PubMed  Google Scholar 

  2. Kelly PA, Binart N, Freemark M, Lucas B, Goffin V, Bouchard B (2001) Prolactin receptor signal transduction pathways and actions determined in prolactin receptor knockout mice. Biochem Soc Trans 29:48–52

    Article  CAS  PubMed  Google Scholar 

  3. Fleenor D, Arumugam R, Freemark M (2006) Growth hormone and prolactin receptors in adipogenesis: STAT-5 activation, suppressors of cytokine signaling, and regulation of insulin-like growth factor I. Horm Res 66:101–110

    Article  CAS  PubMed  Google Scholar 

  4. Clevenger CV, Kline JB (2001) Prolactin receptor signal transduction. Lupus 10:706–718

    Article  CAS  PubMed  Google Scholar 

  5. Barreras Serrano A, Herrera Haro JG, Hori-Oshima S, Gutierrez Espinosa A, Ortega Cerrilla ME, Perez Perez J, Lemus Flores C, Kinejara Espinosa AL, ArangureA Gonzalez, Soto Avila JG (2009) Prolactin receptor (PRLR) gen polymorphism and associations with reproductive traits in Pigs. J Anim Vet Adv 8:469–475

    CAS  Google Scholar 

  6. Tomas A, Casellas J, Ramirez O, Munoz G, Noguera JL, Sanchez A (2006) High amino acid variation in the intracellular domain of the pig prolactin receptor (PRLR) and its relation to ovulation rate and piglet survival traits. J Anim Sci 84:1991–1998

    Article  CAS  PubMed  Google Scholar 

  7. Kmiec M, Terman A (2006) Associations between the prolactin receptor gene polymorphism and reproductive traits of boars. J Appl Genet 47:139–141

    PubMed  Google Scholar 

  8. Terman A (2005) Effect of the polymorphism of prolactin receptor (PRLR) and leptin (LEP) genes on litter size in Polish pigs. J Anim Breed Genet 122:400–404

    Article  CAS  PubMed  Google Scholar 

  9. Van Rens BT, Evans GJ, van der Lende T (2003) Components of litter size in gilts with different prolactin receptor genotypes. Theriogenology 59:915–926

    Article  PubMed  Google Scholar 

  10. Brym P, Kaminski S, Wojcik E (2005) Polymorphism within the bovine prolactin receptor gene (PRLR). Anim Sci Pap Rep 23:61–66

    CAS  Google Scholar 

  11. Viitala S, SzydaJ BlottS, Schulman N, Lidauer M, Maki-Tanila A, Georges M, Vilkki J (2006) The role of the bovine growth hormone receptor and prolactin receptor genes in milk, fat and protein production in Finnish Ayrshire dairy cattle. Genetics 173:2151–2164

    Article  CAS  PubMed  Google Scholar 

  12. Zhang JL, Zan LS, Fang P, Zhang F, Shen G, Tian WQ (2008) Genetic variation of PRLR gene and association with milk performance traits in dairy cattle. Can J Anim Sci 88:33–39

    CAS  Google Scholar 

  13. Ren G, Chen H, Zhang LZ, Lan XY, Wei TB, Li MJ, Jing YJ, Lei CZ, Wang JQ (2010) A coding SNP of LHX4 gene is associated with body weight and body length in bovine. Mol Biol Rep 37:417–422

    Article  CAS  PubMed  Google Scholar 

  14. Orita M, Iwahana H, Kanazawa H, Hayashi K, Sekiya T (1989) Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci 86:2766–2770

    Article  CAS  PubMed  Google Scholar 

  15. Li F, Chen H, Lei CZ, Ren G, Wang J, Li ZJ, Wang JQ (2010) Novel SNPs of the bovine GAD1/gad67 gene and their association with growth traits in three native Chinese cattle breeds. Mol Biol Rep 37:501–505

    Article  CAS  PubMed  Google Scholar 

  16. Jiang RS, Xu GY, Zhang XQ, Yang N (2005) Association of polymorphisms for prolactin and prolactin receptor genes with broody traits in chickens. Poult Sci 84:839–845

    CAS  PubMed  Google Scholar 

  17. Chu MX, Mu YL, Fang L, Ye SC, Sun SH (2007) Prolactin receptor as a candidate gene for prolificacy of small tail han sheep. Anim Biotechnol 18:65–73

    Article  CAS  PubMed  Google Scholar 

  18. Iso-Touru T, Kantanen J, Li MH, Gizejewski Z, Vilkki J (2009) Divergent evolution in the cytoplasmic domains of PRLR and GHR genes in Artiodactyla. BMC Evol Biol 9:172

    Article  PubMed  Google Scholar 

  19. Tast A, Halli O, Ahlstrom S, Andersson H, Love RJ, Peltoniemi OA (2001) Seasonal alterations in circadian melatonin rhythms of the European wild boar and domestic gilt. J Pineal Res 30:43–49

    Article  CAS  PubMed  Google Scholar 

  20. Kelly PA, Binart N, Lucas B, Bouchard B, Goffin V (2001) Implications of multiple phenotypes observed in prolactin receptor knockout mice. Front Neuroendocrinol 22:140–145

    Article  CAS  PubMed  Google Scholar 

  21. Fleenor D, Oden J, Kelly PA, Mohan S, Alliouachene S, Pende M, Wentz S, Kerr J, Freemark M (2005) Roles of the lactogens and somatogens in perinatal and postnatal metabolism and growth: studies of a novel mouse model combining lactogen resistance and growth hormone deficiency. Endocrinology 146:103–112

    Article  CAS  PubMed  Google Scholar 

  22. Nixon AJ, Ford CA, Wildermoth JE, Craven AJ, Ashby MG, Pearson AJ (2002) Regulation of prolactin receptor expression in ovine skin in relation to circulating prolactin and wool follicle growth status. J Endocrinol 172:605–614

    Article  CAS  PubMed  Google Scholar 

  23. Arumugam R, Fleenor D, Freemark M (2007) Lactogenic and somatogenic hormones regulate the expression of neuropeptide Y and cocaine and amphetamine regulated transcript in rat insulinoma (INS-1) cells: interactions with glucose and glucocorticoids. Endocrinology 148:258–267

    Article  CAS  PubMed  Google Scholar 

  24. Vaclavicek A, Hemminki K, Bartram CR, Wagner K, Wappenschmidt B, Meindl A, Schmutzler RK, Klaes R, Untch M, Burwinkel B, Forsti A (2006) Association of prolactin and its receptor gene regions with familial breast cancer. J Clin Endocrinol Metab 91:1513–1519

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 30800847), Natural Science Foundation for Colleges and Universities in Jiangsu Province (No. 08KJD240002), The Key Discipline Fund of Jiangsu Province (No. SYC0703), Natural Science Foundation of Xuzhou Normal University (No. KY2007042), and Qinglan Project of Jiangsu Province.

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Authors and Affiliations

  1. School of Life Sciences, Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou, 221116, China

    Aijun Lü, Xiucai Hu, Hong Chen & Yonghong Pang

  2. College of Animal Science, Henan Institute of Science and Technology, Xinxiang, 453003, China

    Yongjun Dong

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  1. Aijun Lü
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  2. Xiucai Hu
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  3. Hong Chen
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  4. Yongjun Dong
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  5. Yonghong Pang
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Correspondence to Aijun Lü.

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Aijun Lü, Xiucai Hu These authors are joint first authors.

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Lü, A., Hu, X., Chen, H. et al. Single nucleotide polymorphisms of the prolactin receptor (PRLR) gene and its association with growth traits in chinese cattle. Mol Biol Rep 38, 261–266 (2011). https://doi.org/10.1007/s11033-010-0103-5

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