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Identification and characterization of class I chitinase in Panax ginseng C. A. Meyer

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Abstract

The role of plant chitinases in protecting plants against a variety of fungal pathogens is well established. In the present study, a cDNA clone containing a class I chitinase (Chi-1) gene, designated as PgChi-1, has been isolated from the oriental medicinal plant Panax ginseng. PgChi-1 is predicted to encode a protein of 34.9 kDa consisting of 323 amino acid residues. PgChi-1 was found to be expressed constitutively in all of the studied organs of ginseng plant. Under various abiotic stress treatments including Cu, H2O2, mannitol, SA, JA, and NaCl, the expression of PgChi-1 in plantlets and hairy roots increased significantly compared to the control. When different parts of root were analyzed, maximum level was observed in taproot. In addition, levels of PgChi-1 expression were compared between healthy root and fungal, bacterial, and nematode infected root. Significant increase of PgChi-1 was noticed in pathogen infected roots than healthy roots. This study revealed that PgChi-1 may protect the P. ginseng under both biotic and abiotic stress conditions.

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Abbreviations

ABA:

Abscisic acid

SA:

Salicylic acid

JA:

Jasmonic acid

H2O2 :

Hydrogen peroxide

NaCl:

Sodium chloride (salt)

CuSO4 :

Copper sulfate

ORF:

Open reading frame

RT-PCR:

Reverse transcriptase-polymerase chain reaction

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Acknowledgments

This study was supported by KGCMVP for Technology Development Program of Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.

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Authors and Affiliations

  1. Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University, 1 Seocheon, Giheung-Gu Yongin-Si, Gyeonggi-Do, Suwon, 449-701, South Korea

    Rama Krishna Pulla, Ok Ran Lee, Jun-Gyo In, Shohana Parvin, Yu-Jin Kim, Ju-Sun Shim, Hua Sun, Yeon-Ju Kim, Kalaiselvi Senthil & Deok-Chun Yang

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  1. Rama Krishna Pulla
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  2. Ok Ran Lee
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Correspondence to Deok-Chun Yang.

Electronic supplementary material

Below is the link to the electronic supplementary material.

11033_2010_82_MOESM1_ESM.pdf

Supplementary Fig. 1 Nucleotide and deduced amino acid sequences of a class I chitinase cDNA isolated from root of Panax ginseng. The deduced amino acid sequence is shown in single letter code below the nucleotide sequence. Numbers to the right and left refer to numbers of nucleotides. The asterisk denotes the translation stop signal. Poly A signal (AATAAA) was marked in box. Amino acids underlined in single and double lines are the chitinases family 19 signature 1 and 2 respectively. (PDF 47 kb)

11033_2010_82_MOESM2_ESM.pdf

Supplementary Fig. 2 A phylogenetic tree based on chitinase amino acid sequence, showing the phylogenetic relationship between PgChi-1 and other plant’s Chitinases. The tree was constructed using the Clustal X method (Neighbor-joining) and a bar represents 0.05 substitutions per amino acid position. (PDF 15 kb)

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Pulla, R.K., Lee, O.R., In, JG. et al. Identification and characterization of class I chitinase in Panax ginseng C. A. Meyer. Mol Biol Rep 38, 95–102 (2011). https://doi.org/10.1007/s11033-010-0082-6

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  • DOI: https://doi.org/10.1007/s11033-010-0082-6

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