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The influence of ADAR1’s regulation on lymphocyte cell function during rejection

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Abstract

The RNA editing adenosine deaminase gene (ADAR1) expression is wide-spread in lymphocytes. We explore the mechanism of ADAR1 regulation on the function of T primary lymphocytes when rejection occurs by knock-down the expression of ADAR1 in mouse T primary lymphocytes in mixed lymphocyte cultures. The changes of cell proliferation, the expression of ADAR1 and the cell cycle related genes cyclin D1 and A1, cell cycle and apoptosis analysis and mouse gene expression profiles was evaluated. We found that treatment with ADAR1-specific siRNA inhibited allogenic antigen stimulated T cell proliferation, arrested T cell cycle at G0/G1 phases and promoted T cell apoptosis, which was associated with down-regulation of some related key genes transcription. These findings suggest that ADAR1 is essential for the maintenance of function of T lymphocytes during acute rejection. The mechanism underlying ADAR1’s action might include editing of a currently unknown substrate and interacting with other proteins.

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Acknowledgments

This work was supported by Natural Science Foundation of China Grants No. 30271281; No. 30170923; No. 30371399; No. 30571828.

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Authors and Affiliations

  1. Department of Hepato-Biliary Surgery, Xijing Hospital, The Fourth Military Medical University, 710032, Xi’an, China

    Lei Cai, Wei Zhang, Binliang Huo, Wei Zheng, Rui Ding, Qingchuan Zhao & Kefeng Dou

  2. Department of Anesthesia, Xijing Hospital, The Fourth Military Medical University, 710032, Xi’an, China

    Yan Li

  3. Department of Orthopaedics, Shanghai Pudong New Area Gongli Hospital, 200135, Shanghai, China

    Feng Liu

  4. Department of General Surgery, Shaanxi Provincial People’s Hospital, 710068, Xi’an, China

    Binliang Huo & Wei Zheng

  5. Department of Emergency, Xi’an Children’s Hospital, 710002, Xi’an, China

    Jiyuan Guo

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  1. Lei Cai
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  2. Yan Li
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  3. Feng Liu
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  4. Wei Zhang
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Correspondence to Qingchuan Zhao or Kefeng Dou.

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Lei Cai and Yan Li contributed equally to this work.

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Cai, L., Li, Y., Liu, F. et al. The influence of ADAR1’s regulation on lymphocyte cell function during rejection. Mol Biol Rep 37, 2703–2709 (2010). https://doi.org/10.1007/s11033-009-9804-z

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  • DOI: https://doi.org/10.1007/s11033-009-9804-z

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