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Pro-inflammatory cytokines of rat vasculature in DOCA-salt treatment

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Abstract

The purpose of this study was to ascertain the onset expression of pro-inflammatory cytokines in the aorta and kidney and establish their correlation with the increase in arterial blood pressure in rats subjected to DOCA-salt treatment. Male Sprague–Dawley rats underwent unilateral nephrectomy and received subcutaneous DOCA (20 mg/rat/week) as well as 1% NaCl and 0.2% KCl for drinking for 2 weeks. Blood pressure and expression of pro-inflammatory cytokines in aorta and kidney were studied weekly during the induction of hypertension. The treated rats exhibited a mild elevation of blood pressure at 1 week and a profound increase at 2 weeks. Quantitative RT-PCR demonstrated a 4.9-fold and a 3.6-fold enhancement in the expression of TNF-α and IL-6, respectively, in aorta as early as 1 week. The expression of IL-6 and TNF-α in the kidney remained almost unchanged at 1 week but mildly increased at 2 weeks DOCA-salt treatment. This study indicates a robust increase in the expression of IL-6 and TNF-α in aorta in DOCA-salt treated rats. This enhancement suggests that the activation of pro-inflammatory cytokines may contribute to onset of the elevation of blood pressure in DOCA-salt hypertension model.

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Acknowledgments

These studies were supported by a grant from Tehran Medical Sciences University (to MK), and the National Institute of Health Grant DK 62829 (to MS).

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Authors and Affiliations

  1. Department of Physiology, School of Medicine, Tehran University of Medical Sciences, 14155-6447, Tehran, Iran

    Behjat Seifi & Mehri Kadkhodaee

  2. Department of Medicine, University of Cincinnati, Cincinnati, OH, USA

    Jie Xu & Manoocher Soleimani

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  1. Behjat Seifi
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  2. Mehri Kadkhodaee
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Correspondence to Mehri Kadkhodaee.

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Seifi, B., Kadkhodaee, M., Xu, J. et al. Pro-inflammatory cytokines of rat vasculature in DOCA-salt treatment. Mol Biol Rep 37, 2111–2115 (2010). https://doi.org/10.1007/s11033-009-9676-2

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  • DOI: https://doi.org/10.1007/s11033-009-9676-2

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