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Effect of simvastatin on culturing of kidney cells from pigs in vitro

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Abstract

Statins is an inhibitor in the cholesterol biosynthesis pathway. Simvastatin (SIM) has been found to have other clinical benefits besides those resulted from its actions of reducing plasma level of low density lipoprotein (LDL) cholesterol. Both mevastatin (MEV) and parvastatin (PAR) can increase release of nitric oxide (NO) which is a potent inhibitor of platelet aggregation and endothelial cell conglutination. In this study, we found different concentrations of SIM had different effects on 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) mRNA expression and NO and total cholesterol (TC) in normal cultural pig kidney cells. NO and TC were measured by using colorimetry in 550 nm and 546 nm, respectively. HMGR mRNA expression was tested by RT-PCR. Results showed that HMGR mRNA expression had a significant difference (P < 0.05) between different concentration of SIM treatment (0, 5, 10, or 25 μmol/l). HMGR mRNA expression and TC content decreased gradually with the elevation of SIM concentration. The content of NO increased with the elevation of SIM concentration, but the difference was not notable. SIM affects the expression of HMGR-CoA, TC and NO in normal cells, but the specific mechanism need to be further research.

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Acknowledgements

The authors greatly appreciate Beijing Breeding Swine Centre for providing materials and Institute of Animal Science, Chinese Academy of Agricultural Sciences for their cell technical assistance. This study was supported by National Scientific and Technical Supporting Programs of China (No. 2006BAD13B08).

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Authors and Affiliations

  1. College of Animal Science and Technology, China Agricultural University, 100193, Beijing, China

    Guifen Liu, Haichao Lin, Jinlian Fu & Aiguo Wang

  2. Key Laboratory of Animal Genetics and Breeding, Ministry of Agriculture, 2 Yuanmingyuan Xilu, Haidian District, 100193, Beijing, People’s Republic of China

    Guifen Liu, Jinlian Fu & Aiguo Wang

  3. Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, 250100, Jinan, China

    Haichao Lin

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  1. Guifen Liu
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  2. Haichao Lin
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Correspondence to Jinlian Fu or Aiguo Wang.

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Liu, G., Lin, H., Fu, J. et al. Effect of simvastatin on culturing of kidney cells from pigs in vitro. Mol Biol Rep 37, 1391–1395 (2010). https://doi.org/10.1007/s11033-009-9522-6

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