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Molecular characterization of rice OsBIANK1, encoding a plasma membrane-anchored ankyrin repeat protein, and its inducible expression in defense responses

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Abstract

A rice gene, OsBIANK1, encoding a protein containing a typical ankyrin repeat domain, was cloned and identified. The OsBIANK1 protein, consisting of 329 amino acids, contains a conserved ankyrin repeat domain with two ankyrin repeats organized in tandem and was showed to be localized on cytoplasmic membrane during transient expression in onion epidermal cells. Expression of OsBIANK1 was induced by treatment with benzothiadiazole (BTH), a chemical inducer capable of inducing disease resistance response in rice. In BTH-treated rice seedlings, expression of OsBIANK1 was further induced by infection with Magnaporthe grisea, the rice blast fungus, as compared with those in water-treated seedlings. Our preliminary results confirm previous evidences that OsBIANK1 may be involved in regulation of disease resistance response in rice.

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Abbreviations

ANK:

Ankyrin

BTH:

Benzothiadiazole

GFP:

Green fluorescent protein

SA:

Salicylic acid

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Acknowledgments

The authors thank Mr. Rongyao Chai (Zhejiang Academy of Agricultural Science, Hangzhou, China) for the Magnaporthe grisea isolate 85-14B1. This study was supported by National Natural Science Foundation of China (grants no. 30771399), the National “863” Project (2007AA10Z140) and the Ph.D. Programs Foundation of Ministry of Education of China (No. 20070335111).

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Authors and Affiliations

  1. State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, 310029, Hangzhou, Zhejiang, People’s Republic of China

    Xinchun Zhang, Dayong Li, Huijuan Zhang, Xiaoe Wang, Zhong Zheng & Fengming Song

  2. Department of Plant Protection, College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, Zhejiang, People’s Republic of China

    Fengming Song

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  1. Xinchun Zhang
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  2. Dayong Li
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  3. Huijuan Zhang
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Correspondence to Fengming Song.

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Zhang, X., Li, D., Zhang, H. et al. Molecular characterization of rice OsBIANK1, encoding a plasma membrane-anchored ankyrin repeat protein, and its inducible expression in defense responses. Mol Biol Rep 37, 653–660 (2010). https://doi.org/10.1007/s11033-009-9507-5

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