Abstract
Members of the permease gene family are responsible for important biological functions in the growth and development of rice. Here, we show that OsAAP8 is a constitutive expression gene, and its translated protein is localized on the cell membrane. Mutation of the OsAAP8 can promote the expression of genes related to protein and amylopectin synthesis, and also promote the enlargement of protein bodies in its endosperm, leading to an increase in the protein, amylopectin, and total amino acid content of grains in OsAAP8 mutants. Seeds produced by the OsAAP8 mutant were larger, and the chalkiness traits of the OsAAP8 mutants were significantly reduced, thereby improving the nutritional quality and appearance of rice grains. The OsAAP8 protein is involved in the transport of various amino acids; OsAAP8 mutation significantly enhanced the root absorption of a range of amino acids and might affect the distribution of various amino acids. Therefore, OsAAP8 is an important quality trait gene with multiple biological functions, which provides important clues for the molecular design of breeding strategies for developing new high-quality varieties of rice.
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Acknowledgements
This work was financially supported by National Natural Science Foundation of China (U2004141, 31801332; 12305399), Key Project of Science and Technology in Henan Province (222102110141; 222102110115; 212102110249), Key R&D and Promotion Project of Xinyang-Science and Technology Research Project (20230025), Graduate Research Innovation Fund Project of XYNU (2022KYJJ066, 2022KYJJ068), and Nanhu Scholars Program for Young Scholars of XYNU.
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Bo Peng and Quanxiu Wang designed the experiments. Bo Peng, Qingxi Zhang, Yan Liu, Xiaoyu Sun and Xiayu Tian carried out most of the experiments and analyzed the data. Qiang Zhao, Zhiguo Zhang, Yaqin Huang and Juan Peng contributed to the experiments and formal analysis. Yanfang Sun, Xiaohua Song and Guiying Guo contributed to the experiments. Jinhui Zhao, Ruihua Pang and Wei Zhou contributed to the experiments and analyzed the data. Bo Peng and Quanxiu Wang wrote the manuscript.
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Peng, B., Zhang, Q., Liu, Y. et al. OsAAP8 mutation leads to significant improvement in the nutritional quality and appearance of rice grains. Mol Breeding 44, 34 (2024). https://doi.org/10.1007/s11032-024-01473-w
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DOI: https://doi.org/10.1007/s11032-024-01473-w