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Wheat methionine sulfoxide reductase genes and their response to abiotic stress

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Abstract

The wheat cultivar Shanrong no. 3 (cv. SR3) tolerates both salinity and drought stress more effectively than does its progenitor cultivar Jinan 177 (cv. JN177). When the cultivars are subjected to stress, a number of genes encoding methionine sulfoxide reductase (MSRs) are known to be upregulated in SR3. Here, a set of 12 full length Triticum aestivum MSR (TaMSR) cDNAs have been isolated from cv. SR3. The genes were transcribed in the wheat root, stem, and leaf in plants sampled at various developmental stages. Those induced by salinity and drought harbored known stress-responsive cis elements in their promoter region. The constitutive expression in Arabidopsis thaliana of four MSRs which were induced by salt and drought in microarray assay showed that the product of one (TaMSRA2) heightened the plant’s tolerance to NaCl, methylviologen (MV), and abscisic acid, that of the second (TaMSRA5) enhanced salinity tolerance, that of the third (TaMSRB1.1) increased tolerance to salinity, MV and H2O2, and that of the fourth (TaMSRB5.1) increased tolerance to both salinity and mannitol. The effect of the presence in A. thaliana of TaMSRB1.1 was to suppress the accumulation of reactive oxygen species and to increase the intracellular content of soluble sugars.

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Acknowledgements

This research was supported by Natural Science Foundation of China (31471486, 31271706) and agricultural industrialization development project of high-quality seed from Shandong Province (2013). Authors thank former Prof. Robert Koebner in John Innes Centre of the UK for critical comments and language improvement.

Author contribution

FC designed the research. FC and PD wrote the paper. PD, YG and JZ performed the experiments. GX contributed to data analysis. All authors read and approved the manuscript.

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Authors and Affiliations

  1. The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan, 250100, China

    Pengcheng Ding, Yankun Gao, Jiantang Zhu, Fanguo Chen & Guangmin Xia

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  1. Pengcheng Ding
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  2. Yankun Gao
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  3. Jiantang Zhu
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  4. Fanguo Chen
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  5. Guangmin Xia
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Correspondence to Fanguo Chen.

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Ding, P., Gao, Y., Zhu, J. et al. Wheat methionine sulfoxide reductase genes and their response to abiotic stress. Mol Breeding 36, 169 (2016). https://doi.org/10.1007/s11032-016-0597-1

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  • DOI: https://doi.org/10.1007/s11032-016-0597-1

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