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QTLs for resistance to anthracnose identified in two Capsicum sources

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Abstract

Two SNP maps were constructed from two chili populations including Capsicum annuum ‘Bangchang’ × C. chinense ‘PBC932’, and C. baccatum ‘PBC80’ × ‘CA1316’, aiming to identify QTLs for anthracnose resistance. The ‘PBC932’-derived map contained 12 linkage groups (LG) with 214 SNPs and 824 cM coverage. The ‘PBC80’-derived map contained 12 LGs with 403 SNPs and 1270 cM coverage. Based on the ‘PBC932’ map, two QTLs corresponding to the anthracnose resistances in mature green and ripe fruit stages were identified on the same location of LG2 between two SNPs within 14 cM. Based on the ‘PBC80’ map, total five QTLs (three major and two minor QTLs) were identified in the ripe fruit stage, which corresponded to different resistance traits that were assayed by different inoculation methods [microinjection (MI) and high-pressure spray (HP)] with two different pathotypes (PCa2 and PCa3). All the three major QTLs for the resistance traits assayed by PCa2/MI, PCa3/MI, and PCa3/HP were located on LG4 between two SNP markers within 17 cM, while the two minor QTLs for the traits assayed by PCa3/MI and PCa3/HP were on LG12 and LG9 between two SNP markers within 6 and 3 cM, respectively. All the SNP markers flanking the identified QTLs will be helpful for marker-assisted selection for the anthracnose resistance traits in the chili populations derived from ‘PBC932’ and ‘PBC80’.

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Acknowledgments

The project was co-funded by the National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, the East West Seed Company (Thailand), and the Center of Excellence on Agricultural Biotechnology, Science and Technology Postgraduate Education and Research Development, Office of Higher Education Commission, Ministry of Education (AG-BIO/PERDO-CHE). The Royal Golden Jubilee Scholarship, the Thailand Research Fund, granted a Ph.D. scholarship. Updated Colletotrichum taxonomy was provided by Professor Dr. Paul W.J. Taylor, The University of Melbourne.

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Authors and Affiliations

  1. Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand

    Pitchayapa Mahasuk & Orarat Mongkolporn

  2. Center of Excellence on Agricultural Biotechnology (AG-BIO/PERDO-CHE), Bangkok, 10900, Thailand

    Pitchayapa Mahasuk & Orarat Mongkolporn

  3. Department of Biotechnology, East West Seed Company, Chiang Mai, 50290, Thailand

    Darush Struss

  4. Tropical Vegetable Research Center, Department of Horticulture, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand

    Orarat Mongkolporn

Authors
  1. Pitchayapa Mahasuk
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  2. Darush Struss
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  3. Orarat Mongkolporn
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Correspondence to Orarat Mongkolporn.

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Mahasuk, P., Struss, D. & Mongkolporn, O. QTLs for resistance to anthracnose identified in two Capsicum sources. Mol Breeding 36, 10 (2016). https://doi.org/10.1007/s11032-016-0435-5

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