Skip to main content
SpringerLink
Log in

Development of molecular and biochemical tools to investigate fruit quality traits in strawberry elite genotypes

  • Original paper
  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Molecular and biochemical studies were undertaken to elucidate gene/product relationships which influence key fruit quality traits in cultivated strawberry (Fragaria × ananassa Duch.). Comparative transcription profiling experiments in selected genotypes pointed out a number of differentially-expressed genes, possibly related to important fruit quality traits as aroma and fruit firmness. Some of the altered cDNAs encode putative cinnamyl alcohol dehydrogenase, cinnamoyl CoA reductase, cellulase and expansin genes, involved in the early steps of lignin biosynthesis and in cell wall structure, respectively. Parallel biochemical analyses studied the spectra of volatile compounds by Proton Transfer Reaction-Mass Spectrometry (PTR-MS) and alcohol acyl transferase (AAT) specific activity in red fruits. A correlation between the expression of an aat gene, total AAT activity and the presence of related esters in fruit headspace was found.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aharoni A, O’Connell AP (2002) Gene expression analysis of strawberry achene and receptacle maturation using DNA microarrays. J Exp Bot 53:2073–2087

    Article  PubMed  CAS  Google Scholar 

  • Aharoni A, Keizer LCP, Bouwmeester HJ, Sun Z, Alvarez-Huerta M, Verhoeven HA, Blaas J, van Houwelingen AMML, De Vos RCH, van der Voet H, Jansen RC, Guis M, Mol J, Davis RW, Schena M, van Tunen AJ, O’Connell AP (2000) Identification of the SAAT gene involved in strawberry flavor biogenesis by use of DNA microarrays. Plant Cell 12:647–661

    Article  PubMed  CAS  Google Scholar 

  • Aharoni A, Keizer LCP, Van Den Broeck HC, Blanco-Portales R, Munoz-Blanco J, Bois G, Smit P, De Vos RCH, O’Connell AP (2002) Novel insights into vascular, stress, and auxin-dependent and -independent gene expression programs in strawberry, a non-climateric fruit. Plant Physiol 129:1019–1031

    Article  PubMed  CAS  Google Scholar 

  • Anterola AM, Lewis NG (2002) Trends in lignin modification: a comprehensive analysis of the effects of genetic manipulations/mutations on lignification and vascular integrity. Phytochemistry 61:221–294

    Article  PubMed  CAS  Google Scholar 

  • Azodanlou R, Darbellay C, Luisier JL, Villettaz JC, Amadò R (2003) Quality assessment of strawberries (Fragaria species). J Agric Food Chem 51:715–721

    Article  PubMed  CAS  Google Scholar 

  • Benítez-Burraco A, Blanco-Portales R, Redondo-Nevado J, Bellido ML, Moyano E, Caballero J-L, Mun∼oz-Blanco J (2003) Cloning and characterization of two ripening-related strawberry (Fragaria × ananassa cv. Chandler) pectate lyase genes. J Exp Bot 54:633–645

    Article  PubMed  Google Scholar 

  • Biasioli F, Gasperi F, Aprea E, Mott D, Boscaini E, Mayr D, Märk TD (2003) Coupling proton transfer reaction-mass spectrometry with linear discriminant analysis: a case study. J Agric Food Chem 51:7227–7233

    Article  PubMed  CAS  Google Scholar 

  • Biasioli F, Gasperi F, Aprea E, Endrizzi I, Framondino V, Marini F Mott D, Märk TD (2006) Correlation of PTR-MS spectral fingerprints with sensory characterisation of flavour and odour profile of “Trentingrana” cheese. Food Qual Prefer 17:63–75

    Article  Google Scholar 

  • Blanco-Portales R, Medina-Escobar N, Lopez-Raez JA, Gonzalez-Reyes JA, Villalba JM, Moyano E, Caballero JL, Munoz-Blanco J (2002) Cloning, expression and immunolocalization pattern of a cinnamyl alcohol dehydrogenase gene from strawberry (Fragaria × ananassa cv. Chandler). J Exp Bot 53:1723–1734

    Article  PubMed  CAS  Google Scholar 

  • Boelens MH (1996–1999) VCF (2000) Volatile compounds in food, qualitative and quantitative data, 7th edn. (1996), Suppl 1 (1997), Suppl 2 (1999), Huizen, BACIS

  • Boscaini E, Van Ruth S, Biasioli F, Gasperi F, Märk TD (2003) Gas chromatography-olfactometry (GC-O) and proton transfer reaction-mass spectrometry (PTR-MS) analysis of the flavor profile of Grana Padano, Parmigiano Reggiano, and Grana Trentino cheeses. J Agric Food Chem 51:1782–1790

    Article  PubMed  CAS  Google Scholar 

  • Boschetti A, Biasioli F, van Opbergen M, Warneke C, Jordan A, Holzinger R, Prazeller P, Karl T, Hansel A, Lindinger W, Iannotta S (1999) PTR-MS real time monitoring of the emission of volatile organic compounds during postharvest aging of berryfruit. Postharvest Biol Technol 17:143–151

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  • Buhr K, van Ruth S, Delahunty C (2002) Analysis of volatile flavour compounds by Proton-Transfer Reaction-Mass Spectrometry: fragmentation patterns and discrimination between isobaric and isomeric compounds. Int J Mass Spectrom 221:1–7

    Article  CAS  Google Scholar 

  • Carbone F, Pizzichini D, Giuliano G, Rosati C, Perrotta G (2005) Comparative profiling of tomato fruits and leaves evidences a complex modulation of global transcript profiles. Plant Sci 169:165–175

    Article  CAS  Google Scholar 

  • Civello PM, Powell ALT, Sabehat A, Bennett AB (1999) An expansin gene expressed in ripening strawberry fruit. Plant Physiol 121:1273–1279

    Article  PubMed  CAS  Google Scholar 

  • Cleveland W, Devlin S (1998) Locally weighted linear regression: an approach to regression analysis by local fitting. J Am Stat Assoc 83:596–609

    Article  Google Scholar 

  • Davis TM, Yu H (1997) A linkage map of the diploid strawberry, Fragaria vesca. J Hered 88:215–221

    CAS  Google Scholar 

  • Dorfner R, Ferge T, Yeretzian C, Kettrup A, Zimmermann R (2004) Laser mass spectrometry as on-line sensor for industrial process analysis: process control of coffee roasting. Anal Chem 76:1386–1402

    Article  PubMed  CAS  Google Scholar 

  • El-Sharkawy I, Manríquez D, Flores FB, Regad F, Bouzayen M, Latché A, Pech J-C (2005) Functional characterization of a melon alcohol acyl-transferase gene family involved in the biosynthesis of ester volatiles. Identification of the crucial role of a threonine residue for enzyme activity. Plant Mol Biol 59:345–362

    Article  PubMed  CAS  Google Scholar 

  • Fei Z, Tang X, Alba RM, White JA, Ronning CM, Martin GB, Tanksley SD, Giovannoni JJ (2004) Comprehensive EST analysis of tomato and comparative genomics of fruit ripening. Plant J 40:47–59

    Article  PubMed  Google Scholar 

  • Fiehn O (2002) Metabolomics – the link between genotypes and phenotypes. Plant Mol Biol 48:155–171

    Article  PubMed  CAS  Google Scholar 

  • Fonseca S, Hackler L Jr, Zvara A, Ferreira S, Baldé A, Dudits D, Pais MS, Puskás LG (2004) Monitoring gene expression along pear fruit development, ripening and senescence using cDNA microarrays. Plant Sci 167:457–469

    Article  CAS  Google Scholar 

  • Gasperi F, Gallerani G, Boschetti A, Biasioli F, Monetti A, Boscaini E, Jordan A, Lindinger W, Iannotta S (2001) The mozzarella cheese flavour profile: a comparison between judge panel analysis and proton transfer reaction mass spectrometry. J Sci Food Agric 81:357–363

    Article  CAS  Google Scholar 

  • Hansel A, Jordan A, Warneke C, Holzinger R, Wisthaler A, Lindinger W (1999) Proton-transfer-reaction mass spectrometry (PTR-MS): on-line monitoring of volatile organic compounds at volume mixing ratios of a few pptv. Plasma Sources Sci Technol 8:332–336

    Article  CAS  Google Scholar 

  • Lerceteau-Köhler E, Guerin G, Laigret F, Denoyes-Rothan B (2003) Characterization of mixed disomic and polysomic inheritance in the octoploid strawberry (Fragaria × ananassa) using AFLP mapping. Theor Appl Genet 107:619–628

    Article  PubMed  Google Scholar 

  • Li D, Xu Y, Xu G, Gu L, Li D, Shu H (2006) Molecular cloning and expression of a gene encoding alcohol acyltransferase (MdAAT2) from apple (cv. Golden Delicious). Phytochemistry 67:658–667

    Article  PubMed  CAS  Google Scholar 

  • Lindinger W, Hansel A, Jordan A (1998) On-line monitoring of volatile organic compounds at pptv level by means of Proton-Transfer-Reaction Mass Spectrometry (PTR-MS). Medical application, food control and environmental research. International Int J Mass Spectrom Ion Process 173:191–241

    Article  CAS  Google Scholar 

  • Lloup-Tous I, Domínguez-Puigjaner E, Palomer X, Vendrell M (1999) Characterization of two divergent endo-β-1,4-glucanase cDNA clones highly expressed in the nonclimacteric strawberry fruit. Plant Physiol 119:1415–1421

    Article  Google Scholar 

  • Lopez-Gomez R, Gomez-Lim MA (1992) A method for extracting intact RNA from fruit rich in polysaccharides using ripe mango mesocarp. HortScience 27:440–442

    CAS  Google Scholar 

  • Manning K (1998) Isolation of a set of ripening-related genes from strawberry: their identification and possible relationship to fruit quality traits. Planta 205:622–631

    Article  PubMed  CAS  Google Scholar 

  • Martínez GA, Chaves AR, Civello PM (2004) β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripening. Plant Physiol Biochem 42:89–96

    Article  PubMed  Google Scholar 

  • Marty I, Douat C, Tichit L, Jungsup K, Leustek T, Albagnac G (2000) The cystathionine-β-synthase gene involved in methionine biosynthesis is highly expressed and auxin-repressed during wild strawberry (Fragaria vesca L.) fruit ripening. Theor Appl Genet 100:1129–1136

    Article  CAS  Google Scholar 

  • Medina-Escobar N, Cárdenas J, Moyano E, Caballero JL, Muñoz-Blanco J (1997) Cloning, molecular characterization and expression pattern of a strawberry ripening-specific cDNA with sequence homology to pectate lyase from higher plants. Plant Mol Biol 34:867–877

    Article  PubMed  CAS  Google Scholar 

  • Ménager I, Jost M, Aubert C (2004) Changes in physicochemical characteristics and volatile constituents of strawberry (cv. Cigaline) during maturation. J Agric Food Chem 52:1248–1254

    Article  PubMed  Google Scholar 

  • Mott D, Biasioli F, Gasperi F, Aprea E, Marini F, Märk TD (2004) Characterisation of strawberry genotypes by PTRMS spectral fingerprinting. Acta Horticult 649:65–68

    Google Scholar 

  • Nam YW, Tichit L, Leperlier M, Cuerq B, Marty I, Lelièvre JM (1999) Isolation and characterization of mRNAs differentially expressed during ripening of wild strawberry (Fragaria vesca L.) fruits. Plant Mol Biol 39:629–636

    Article  PubMed  CAS  Google Scholar 

  • Olías R, Perez AG, Sanz C (2002) Catalytic properties of alcohol acyltransferase in different strawberry species and cultivars. J Agricult Food Chem 50:4031–4036

    Article  Google Scholar 

  • Pérez AG, Sanz C, Olías JM (1993) Partial purification and some properties of alcohol acyltransferase from strawberry fruits. J Agric Food Chem 41:1462–1466

    Article  Google Scholar 

  • Pérez AG, Sanz C, Olías R, Ríos JJ, Olías JM (1996) Evolution of strawberry alcohol acyltransferase activity during fruit development and storage. J Agric Food Chem 44:3286–3290

    Article  Google Scholar 

  • Pérez AG, Olías R, Luaces P, Sanz C (2002) Biosynthesis of strawberry aroma compounds through amino acid metabolism. J Agric Food Chem 50:4037–4042

    Article  PubMed  Google Scholar 

  • Rosli HG, Civello PM, Marti’nez GA (2004)Changes in cell wall composition of three Fragaria × ananassa cultivars with different softening rate during ripening. Plant Physiol Biochem 42:823–831

    Article  PubMed  CAS  Google Scholar 

  • Salentijn EMJ, Aharoni A, Schaart JG, Boone MJ, Krens FA (2003) Differential gene expression analysis of strawberry cultivars that differ in fruit-firmness. Physiol Plant 118:571–578

    Article  CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor, New York

    Google Scholar 

  • Sargent DJ, Davis TM, Tobutt KR, Wilkinson MJ, Battey NH, Simpson DW (2004) A genetic linkage map of microsatellite, gene-specific and morphological markers in diploid Fragaria. Theor Appl Genet 109(7):1385–1391

    Article  PubMed  CAS  Google Scholar 

  • Schadt EE, Monks SA, Drake TA, Lusisk AJ, Chek N, Colinayok V, Ruff TG, Milligan SB, Lamb JR, Cavet G, Linsley PS, Mao M, Stoughton RB, Friend SH (2003) Genetics of gene expression surveyed in maize, mouse and man. Nature 422:297–302

    Article  PubMed  CAS  Google Scholar 

  • Trainotti L, Spinello R, Piovan A, Spolaore S, Casadoro G (2001) β-Galactosidases with a lectin-like domain␣are expressed in strawberry. J Exp Bot 52:1635–1645

    Article  PubMed  CAS  Google Scholar 

  • Trainotti L, Zanin D, Casadoro G (2003) A cell wall-oriented genomic approach reveals a new and unexpected complexity of the softening in peaches. J Exp Bot 54:1821–1832

    Article  PubMed  CAS  Google Scholar 

  • Trainotti L, Bonghi C, Ziliotto F, Zanin D, Rasori A, Casadoro G, Ramina A, Tonutti P (2006) The use of microarray to investigate transcriptome changes during transition from pre-climacteric to climacteric phase in peach fruit. Plant Sci 170:606–613

    Article  CAS  Google Scholar 

  • Tsuchikawa S, Hamada T (2004) Application of time-of-flight near infrared spectroscopy for detecting sugar and acid contents in apples. J Agric Food Chem 52:2434–2439

    Article  PubMed  CAS  Google Scholar 

  • Waters DLE, Holton TA, Ablett EM, Lee LS, Henry RJ (2005) cDNA microarray analysis of developing grape (Vitis vinifera cv. Shiraz) berry skin. Funct Integr Genomics 5(1):40–58

    Article  PubMed  CAS  Google Scholar 

  • Yamada K, Lim J, Dale JM, Chen H, Shinn P, Palm CJ, Southwick AM, Wu HC, Kim C, Nguyen M, Pham P, Cheuk R, Karlin-Newmann G, Liu SX, Lam B, Sakano H, Wu T, Yu G, Miranda M, Quach HL, Tripp M, Chang CH, Lee JM, Toriumi M, Chan MMH, Tang CC, Onodera CS, Deng JM, Akiyama K, Ansari Y, Arakawa T, Banh J, Banno F, Bowser L, Brooks S, Carninci P, Chao Q, Choy N, Enju A, Goldsmith AD, Gurjal M, Hansen NF, Hayashizaki Y, Johnson-Hopson C, Hsuan VW, Iida K, Karnes M, Khan S, Koesema E, Ishida J, Jiang PX, Jones T, Kawai J, Kamiya A, Meyers C, Nakajima M, Narusaka M, Seki M, Sakurai T, Satou M, Tamse R, Vaysberg M, Wallender EK, Wong C, Yamamura Y, Yuan S, Shinozaki K, Davis RW, Theologis A, Ecker JR (2003) Empirical analysis of transcriptional activity in the Arabidopsis genome. Science 302:842–846

    Article  PubMed  CAS  Google Scholar 

  • Zini E, Biasioli F, Gasperi F, Mott D, Aprea E, Märk TD, Patocchi A, Gessler C, Komjanc M (2005) QTL mapping of volatile compounds in ripe apples detected by Proton Transfer Reaction-Mass Spectrometry. Euphytica 145:269–279

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Work supported by the Italian MIUR QualiFraPe project, the Austrian FWF of Vienna, and the European ComMission, We thank ISF staff for data on quality traits, F. Giorno for her assistance.

Author information

Authors and Affiliations

  1. ENEA, Trisaia Research Center, S.S. 106, km 419+500, 75026, Rotondella, MT, Italy

    Fabrizio Carbone, Fabienne Mourgues, Carlo Rosati & Gaetano Perrotta

  2. Istituto Agrario di S. Michele all’Adige, Via E. Mach, 2, 38010, S. Michele, TN, Italy

    Franco Biasioli & Flavia Gasperi

  3. Institut für Ionenphysik, Universität Innsbruck, Technikerstr. 25, A-6020, Innsbruck, Austria

    Tilmann D. Märk

Authors
  1. Fabrizio Carbone
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fabienne Mourgues
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Franco Biasioli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Flavia Gasperi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tilmann D. Märk
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Carlo Rosati
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Gaetano Perrotta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gaetano Perrotta.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Carbone, F., Mourgues, F., Biasioli, F. et al. Development of molecular and biochemical tools to investigate fruit quality traits in strawberry elite genotypes. Mol Breeding 18, 127–142 (2006). https://doi.org/10.1007/s11032-006-9017-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11032-006-9017-2

Keywords

Search

Navigation