Abstract
Ischemic stroke (IS) is the most common type of stroke and is characterized by high rates of mortality and long-term injury. The prediction and early diagnosis of IS are therefore crucial for optimal clinical intervention. Proteomics has provided important techniques for exploring protein markers associated with IS, but there has been no systematic evaluation and review of research that has used these techniques. Here, we review the differential proteins that have been found in cell- and animal- based studies and clinical trials of IS in the past 10 years; determine the key pathological proteins that have been identified in clinical trials; summarize the target proteins affected by interventions aimed at treating IS, with a focus on traditional Chinese medicine treatments. Overall, we clarify findings and problems that have been identified in recent proteomics research on IS and provide suggestions for improvements in this area. We also suggest areas that could be explored for determining the pathogenesis and developing interventions for IS.
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Data availability
The datasets supporting the conclusions of this article are available from the corresponding author.
Abbreviations
- BBB:
-
Blood brain barrier
- CSF:
-
Cerebrospinal fluid
- DDA:
-
Data dependent analysis
- DIA:
-
Data independent analysis
- DIGE:
-
Differential gel electrophoresis
- ELISA:
-
Enzyme-linked immunosorbent assay
- ELVO:
-
Emergent large vessel occlusion
- GO:
-
Gene Ontology
- IPSCs:
-
Induced pluripotent stem cells
- IS:
-
Ischemic stroke
- I-R:
-
Ischemia-reperfusion
- iTRAQ:
-
Isobaric tag for relative and absolute quantitation
- KEGG:
-
Kyoto Encyclopedia of Genes and Genomes
- LC:
-
Liquid chromatograph
- MCAO:
-
Middle cerebral artery occlusion
- MRM:
-
Multiple reaction monitoring
- MS:
-
Mass spectrometry
- MSCs:
-
Mesenchymal stem cells
- PEA:
-
Proximity extension assay
- PPI:
-
Protein-protein interaction
- PRM:
-
Parallel reaction monitoring
- PTM:
-
Post-translational modification
- qPCR:
-
Real-time quantitative polymerase chain reaction
- SRM:
-
Selected reaction monitoring
- TCM:
-
Traditional Chinese medicine
- TMT:
-
Tandem mass tag
- t-PA:
-
Tissue plasminogen activator
- WB:
-
Western blot
- 2DE/2D-PAGE:
-
Two-dimensional gel electrophoresis
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The study was supported by grants from by National Natural Science Foundation of China (82173648), the Medical and Health Science and Technology Project in Zhejiang province (2023KY1136), Ningbo Hwa Mei Research Fund (2022HMZY101, 2023HMZD01 and 2021HMKY14).
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Tian Zhao, Yuyi Sha and Liyuan Han contributed to the study conception and design. Tian Zhao wrote first draft of the manuscript. Tian Zhao and Liyuan Han revised and edited the manuscript. All authors commented on previous versions of the manuscript and all authors read and approved the final manuscript.
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Zhao, T., Zeng, J., Zhang, R. et al. Proteomic advance of ischemic stroke: preclinical, clinical, and intervention. Metab Brain Dis 38, 2521–2546 (2023). https://doi.org/10.1007/s11011-023-01262-y
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DOI: https://doi.org/10.1007/s11011-023-01262-y