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Endogenous expression of the atypical chemokine receptor CCX-CKR (CCRL1) gene in human embryonic kidney (HEK 293) cells

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Abstract

CCX-CKR (CCRL1) as one of the chemokine receptor-like proteins is a scavenger of CCL19, CCL21, CCL25, and CXCL13 chemokines. Human CCX-CKR is expressed in various tissues. Since HEK 293 cells are used for both transient and stable expression of CCX-CKR gene, it is important to determine endogenous expression of CCX-CKR gene. Therefore, in the current study endogenous expression of CCX-CKR gene was evaluated in HEK 293 cells. To test the expression of CCX-CKR gene in HEK 293 cells, total RNA was isolated from HEK 293 cells and RT-PCR reaction was primed with the gene-specific primers. Protein expression is then evaluated by Western blot analysis and flow cytometry. Results of this study show that HEK 293 cells express an endogenous CCRL1 gene only at mRNA level. These data therefore represent the important implications for the use of HEK 293 cells as a host cell system for the study of CCX-CKR.

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Acknowledgments

This work was supported by the Grant from University of Isfahan (No. 90/1041).

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran

    Parvin Salimi & Abolghasem Esmaeili

  2. Department of Cardiology, Isfahan University of Medical Sciences, Isfahan, Iran

    Mohammad Hashemi

  3. Heart Failure Research Center, Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran

    Mohaddeseh Behjati

  4. Cell and Molecular Biology Division, Department of Biology, Faculty of Sciences, University of Isfahan, P.O. Box:8174673441, Isfahan, Iran

    Abolghasem Esmaeili

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  1. Parvin Salimi
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  2. Abolghasem Esmaeili
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Correspondence to Abolghasem Esmaeili.

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Salimi, P., Esmaeili, A., Hashemi, M. et al. Endogenous expression of the atypical chemokine receptor CCX-CKR (CCRL1) gene in human embryonic kidney (HEK 293) cells. Mol Cell Biochem 412, 229–233 (2016). https://doi.org/10.1007/s11010-015-2629-2

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  • DOI: https://doi.org/10.1007/s11010-015-2629-2

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