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Changes in plasma thiol levels induced by different phases of treatment in breast cancer; the role of commercial extract from black chokeberry

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Abstract

Different low-molecular-weight thiols, including glutathione, cysteine, and cysteinylglycine are physiological free radical scavengers. On the other hand, homocysteine may play a role as an oxidant. The aim of our present study was to establish in vitro the effects of the commercial extract of Aronia melanocarpa (Aronox®) on the amount of selected low-molecular-weight thiols and the activity of antioxidative enzymes (superoxide dismutase, glutathione peroxidase, and glutathione reductase) in plasma obtained from patients with invasive breast cancer during different phases of treatment [before or after the surgery and patients after different phases of chemotherapy (doxorubicin and cyclophosphamide)] and from healthy subjects. Patients were hospitalized in Department of Oncological Surgery and Department of Chemotherapy, Medical University of Lodz, Poland. The level of low-molecular-weight thiols was determined by high-performance liquid chromatography. We observed that in the presence of the Aronia extract changes in amount of thiols in plasma from breast cancer patients (at all tested groups) were significantly reduced. Our results showed that tested commercial extract reduced modifications of antioxidative enzymes activity in plasma from patients during different phases of treatment, but this effect was not statistical significant. Our results suggest that the Aronia extract supplementation in breast cancer patients has a beneficial effect on thiols concentration in plasma. Plasma, as reported in this work, could be used as an experimental model to evaluate the beneficial action of plant supplements, including phenolic extracts on thiols or other molecules during different phases of treatment.

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Acknowledgments

Supported by the Grant 506/810 from University of Lodz, Poland.

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None to declare.

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Authors and Affiliations

  1. The Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/3, 90-236, Lodz, Poland

    Magdalena Kędzierska & Beata Olas

  2. The Department of Environmental Chemistry, University of Lodz, Pomorska 163, 90-236, Lodz, Poland

    Rafał Głowacki

  3. The Department of Chemotherapy, Medical University of Lodz, Copernicus Memorial Hospital, Lodz, Poland

    Magdalena Kędzierska, Urszula Czernek, Katarzyna Szydłowska-Pazera & Piotr Potemski

  4. The Department of Surgical Oncology, Medical University of Lodz, Copernicus Memorial Hospital, Lodz, Poland

    Janusz Piekarski & Arkadiusz Jeziorski

Authors
  1. Magdalena Kędzierska
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  2. Rafał Głowacki
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  3. Urszula Czernek
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  6. Janusz Piekarski
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  7. Arkadiusz Jeziorski
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  8. Beata Olas
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Correspondence to Beata Olas.

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Kędzierska, M., Głowacki, R., Czernek, U. et al. Changes in plasma thiol levels induced by different phases of treatment in breast cancer; the role of commercial extract from black chokeberry. Mol Cell Biochem 372, 47–55 (2013). https://doi.org/10.1007/s11010-012-1444-2

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  • DOI: https://doi.org/10.1007/s11010-012-1444-2

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