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Identification and functional characterization of a putative 17β-hydroxysteroid dehydrogenase 12 in abalone (Haliotis diversicolor supertexta)

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Abstract

The 17β-hydroxysteroid dehydrogenases (17β-HSDs) are key enzymes in the downstream process of steroid hormone biosynthesis. To date, relatively little is known about the role of 17β-HSDs in marine gastropods. In the present study, a putative cDNA sequence encoding type 12 17β-HSD (17β-HSD-12) was identified in abalone (Haliotis diversicolor supertexta). The full-length cDNA was 1,978 bp, including an open reading frame (ORF) of 963 bp that encoded a protein of 321 amino acids. Comparative structural analysis revealed that abalone 17β-HSD-12 shared 39.8–42.8% amino acid identity with other 17β-HSD-12 homologues and that the functional domains were well conserved. Phylogenetic analysis revealed that abalone 17β-HSD-12 belonged to the short-chain dehydrogenases/reductases (SDRs) family. Functional analysis following transient transfection of the ORF in human embryonic kidney-293 (HEK-293) cells indicated that abalone 17β-HSD-12 had the ability to convert estrone (E1) into estradiol (E2). Expression analysis in vivo demonstrated that abalone 17β-HSD-12 was differentially expressed during the three reproductive stages (pre-spawning, spawning, and post-spawning). These results indicate that abalone 17β-HSD-12 is an SDR family member with a key role in steroidogenesis during the reproductive period.

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Abbreviations

17β-HSD 12:

17β-Hydroxysteroid dehydrogenase 12

SDRs:

Short-chain dehydrogenases/reductases

E1:

Estrone

E2:

Estradiol

ORF:

Open reading frames

HEK-293:

Human embryonic kidney-293 cells

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Acknowledgment

This study was supported by International Cooperative Project of SZISTIC Committee of Shenzhen (ZYA200903260036A), the PhD Start-up Fund of Natural Science Foundation of Guangdong Province, China (10451805702004177), and the Seed Foundation of Graduate School at Shenzhen of Tsinghua University.

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  1. Life Sciences Division, Graduate School at Shenzhen, Campus of Tsinghua University, Shenzhen, Room 304, Building L, University Town, Guangdong Province, People’s Republic of China

    Jin Zhou, Yun-Feng Gao, Lei Li, Hong-Ning Zhai, Shang-Jin Tan & Zhong-Hua Cai

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  1. Jin Zhou
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  2. Yun-Feng Gao
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Correspondence to Zhong-Hua Cai.

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Jin Zhou and Yun-Feng Gao are contributed equally to this work.

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Zhou, J., Gao, YF., Li, L. et al. Identification and functional characterization of a putative 17β-hydroxysteroid dehydrogenase 12 in abalone (Haliotis diversicolor supertexta). Mol Cell Biochem 354, 123–133 (2011). https://doi.org/10.1007/s11010-011-0811-8

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