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Association between circulating interleukin-1 beta (IL-1β) levels and IL-1β C–511T polymorphism with cervical cancer risk in Egyptian women

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Abstract

Cancer cervix is one of the leading causes of cancer-related mortality among women worldwide. It is believed that the host genetic factors such as inflammation-induced cytokines may play a role in cervical carcinogenesis. The interleukin-1β (IL-1β) gene contains several single nucleotide polymorphisms. One of them, C–511T, which in the promoter region has been associated with increased IL-1β production and with increased risk of developing cancers. We assessed the association between the IL-1β C–511T polymorphism and cervical cancer risk in a case–control study among 100 histopathologically confirmed Egyptian women with cervical cancer and 50 age-matched, cervical cytology negative, healthy controls by polymerase chain reaction-restriction fragment length polymorphism. Plasma levels of IL-1β were assayed by enzyme-linked immunosorbent assay. There was significant increase in the mean plasma IL-1β level in cervical cancer cases (43.40 ± 25.95 pg/ml) when compared with controls (30.51 ± 18.28 pg/ml, P = 0.002). The plasma levels above the 75th percentile of controls (IL-1β ≥ 45.74 pg/ml) were significantly associated with a 2.49-fold increased risk of cervical cancer. The significant increase in IL-1β concentration in cervical cancer cases was observed only among cervical cancer cases carrying C–511T variant genotypes. T/T genotype of IL-1β polymorphism was significantly higher in cervical cancer cases compared with controls (57 vs. 38%; OR = 2.16; P = 0.028) and the T allele carriage was significantly associated with cervical cancer risk (OR = 2.00, 95% CI = 1.19–3.38, and P = 0.008). In conclusion, plasma IL-1β level and IL-1β C–511T polymorphism may be considered as candidate biomarkers for cervical cancer in Egyptian women.

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Acknowledgment

The authors are grateful to Dr Bahaa ElDin Hasan for his skilled technical assistance.

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Authors and Affiliations

  1. Department of Medical Biochemistry, Zagazig University, Zagazig, 44519, Egypt

    Magdy A. Al-Tahhan & Rasha L. Etewa

  2. Department of Obstetrics and Gynecology, Zagazig University, Zagazig, 44519, Egypt

    Manal M. El Behery

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  1. Magdy A. Al-Tahhan
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  2. Rasha L. Etewa
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Correspondence to Rasha L. Etewa.

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Al-Tahhan, M.A., Etewa, R.L. & El Behery, M.M. Association between circulating interleukin-1 beta (IL-1β) levels and IL-1β C–511T polymorphism with cervical cancer risk in Egyptian women. Mol Cell Biochem 353, 159–165 (2011). https://doi.org/10.1007/s11010-011-0782-9

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  • DOI: https://doi.org/10.1007/s11010-011-0782-9

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