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Alterations in the expression of genes related to NF-κB signaling in liver and kidney of CuZnSOD-deficient mice

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Abstract

NF-κB signaling pathway plays a central role in regulation of the cellular response to stress. Among numerous factors that modulate NF-κB dependent transcription, reactive oxygen species attracted special attention. In the present work, we compared the expression of 84 genes related to NF-κB signaling between cytosolic superoxide dismutase (CuZnSOD)-deficient and wild-type mice. In kidney, we found seven genes which expression was significantly affected by CuZnSOD deficiency. Among them, four were up-regulated, Egr1, Fos, Il1b, Tnfrsf10b, and three down-regulated, Card10, Ikbkb, Tgfbr2. In the case of liver, six genes were up-regulated, Fos, Il1b, Il1r1, Jun, Tlr7, Tnfrsf10b, and five down-regulated, Casp8, Ikbke, Irak1, Nfkb1, Raf1. The results demonstrate that CuZnSOD deficiency has a significant impact on the expression of NF-κB related genes in both kidney and liver. The differences in gene expression reported in our work may contribute to understanding of the molecular mechanisms underlying phenotypic abnormalities in CuZnSOD-deficient mice, e.g., increase in the incidence of liver cancer.

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Acknowledgments

The authors thank Professor Irena Szumiel for critical reading of the paper and Dr. Paweł Lipiński for providing the animal samples gathered during realization of a grant from Ministry of Science and Higher Education 6P04A 00620 and helpful discussion. This work was supported by a grant from Ministry of Science and Higher Education N301 2052 33.

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Authors and Affiliations

  1. Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195, Warsaw, Poland

    Kamil Brzóska, Barbara Sochanowicz & Marcin Kruszewski

  2. Department of Clinical Biochemistry, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Karłowicza 24, 85-092, Bydgoszcz, Poland

    Agnieszka Siomek & Ryszard Oliński

  3. Independent Laboratory of Molecular Biology, Institute of Agricultural Medicine, Jaczewskiego 2, 20-090, Lublin, Poland

    Marcin Kruszewski

Authors
  1. Kamil Brzóska
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  2. Barbara Sochanowicz
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  3. Agnieszka Siomek
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  4. Ryszard Oliński
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  5. Marcin Kruszewski
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Correspondence to Kamil Brzóska.

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Brzóska, K., Sochanowicz, B., Siomek, A. et al. Alterations in the expression of genes related to NF-κB signaling in liver and kidney of CuZnSOD-deficient mice. Mol Cell Biochem 353, 151–157 (2011). https://doi.org/10.1007/s11010-011-0781-x

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  • DOI: https://doi.org/10.1007/s11010-011-0781-x

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