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Effect of hepatocyte growth factor (HGF) on the level of Survivin & XIAP expression in several human cancer cell lines, after treating with DNA damaging agent

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Abstract

Hepatocyte growth factor (HGF) has opposite biological activities in regulating apoptosis, also underlying molecular mechanisms are not clearly defined. We investigated HGF ability to inhibit cell death, which was induced by Doxorubicin, a DNA damaging agent. Also Survivin and XIAP mRNA levels were compared in HGF treated and non-treated cells. Cell proliferation and death were assessed using MTT assay and dye exclusion tests. Quantitative real-time PCR was used to evaluate Survivin and XIAP expression levels after treatment with HGF. ELISA was performed to quantify HGF secretion in the selected cancer cell lines media. HGF appeared to have inhibitory effect on Doxorubicin induced cell death in all of the studied cell lines. It had minimal effect on XAIP and Survivin expression levels in MRC-5, MOLT-4 and AGS cell lines; except for XIAP expression level in AGS cell line, which was increased substantially after treatment. Surprisingly, in KG-1 cell line, XIAP and Survivin expression levels were significantly reduced after HGF treatment. Although several members of IAP gene family are reported to play role in HGF mediated cytoprotective pathway, we showed that XIAP and Survivin do not seem to be involved.

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Acknowledgements

We are grateful to Professor Cantely LG (Department of Internal Medicine and Section of Nephrology, Yale University) for kindly providing recombinant HGF. Also we thank Professor Reed JC and Steve Hourmezian from Burnham Institute, Professor H Nakshatri (Department of Biochemistry and Molecular Biology, Indiana University) for their kind offers, Dr. Amanzade A and Jalal Radfar for their skilled help in cell culture work, Dr. Mahboudi F (and all members of HIV unit, Pasteur Institute of Iran) and Dr Vaziri for their thoughtful supports.

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Authors and Affiliations

  1. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran

    Kianoosh Keyhanian & Mohammad Ali Shokrgozar

  2. HIV Unit, Department of Biotechnology, Pasteur Institute of Iran, Tehran, Iran

    Kianoosh Keyhanian, Rosita Edalat, Nayere Askary & Ramin Sarrami-Forooshani

  3. Medical Students Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

    Kianoosh Keyhanian & Arghavan Golshani

  4. H-pylori Unit, Department of Biotechnology, Pasteur Institute of Iran, Tehran, Iran

    Akbar Oghalaei

  5. Department of Genetics and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran

    Mansour Salehi

Authors
  1. Kianoosh Keyhanian
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  2. Rosita Edalat
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  3. Akbar Oghalaei
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  4. Nayere Askary
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  5. Arghavan Golshani
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  7. Ramin Sarrami-Forooshani
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  8. Mohammad Ali Shokrgozar
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Correspondence to Mohammad Ali Shokrgozar.

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Keyhanian, K., Edalat, R., Oghalaei, A. et al. Effect of hepatocyte growth factor (HGF) on the level of Survivin & XIAP expression in several human cancer cell lines, after treating with DNA damaging agent. Mol Cell Biochem 304, 199–205 (2007). https://doi.org/10.1007/s11010-007-9500-z

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  • DOI: https://doi.org/10.1007/s11010-007-9500-z

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