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The NADPH- and iron-dependent lipid peroxidation in human placental microsomes

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Abstract

In pregnant females, placenta is the most important source of lipid hydroperoxides and other reactive oxygen species (ROS). The increased production of lipid peroxides is often linked to preeclampsia. In our study, we revealed that NADPH- and iron-dependent lipid peroxidation in human placental microsomes (HPM) occurred. In the presence of Fe2+ ion, HPM produced small amounts of thiobarbituric acid-reactive substances (TBARS) – a final product of lipid peroxidation. NADPH caused a strong increase of iron stimulated TBARS formation. TBARS formation was inhibited by superoxide dismutase, butylated hydroxytoluene and α-tocopherol but not by mannitol or catalase. TBARS and superoxide radical production was inhibited in similar manner by cytochrome P450 inhibitors. The results obtained led us to the following conclusions: (1) microsomal lipid peroxidation next to mitochondrial lipid peroxidation may by an important source of lipid hydroperoxides in blood during pregnancy and (2) superoxide radical released by microsomal cytochrome P450 is an important factor in NADPH- and iron-dependent lipid peroxidation in HPM.

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Acknowledgments

We are indebted to Professor M. M. Żydowo for critical reading and discussion of the manuscript. This work was supported by a grant from Medical University of Gdańsk, within the project W-48 and a grant from KBN, within the project ST-40.

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Authors and Affiliations

  1. Department of Pharmaceutical Biochemistry, Medical University of Gdańsk, ul. Dębinki 1, 80-211, Gdańsk, Poland

    Ryszard Milczarek, Ewa Sokolowska, Anna Hallmann & Jerzy Klimek

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  1. Ryszard Milczarek
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  2. Ewa Sokolowska
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  3. Anna Hallmann
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  4. Jerzy Klimek
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Correspondence to Ryszard Milczarek.

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Milczarek, R., Sokolowska, E., Hallmann, A. et al. The NADPH- and iron-dependent lipid peroxidation in human placental microsomes. Mol Cell Biochem 295, 105–111 (2007). https://doi.org/10.1007/s11010-006-9279-3

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  • DOI: https://doi.org/10.1007/s11010-006-9279-3

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