Abstract
Sea cucumber (Holothuria leucospilota) is a native species of Echinodermata that has long been used as traditional Asian medicine. This study was conducted for the evaluation of the antioxidant property of hydrolyzed protein with molecular weight of < 30-KDa extracted from H. leucospilota using Alcalase and Flavourzyme enzymes. Antioxidant activity was evaluated by DPPH radical scavenging assay and ferric reducing antioxidant power. The results of antioxidant experiments showed that with increasing concentration of hydrolyzed protein (both enzymes), the percentage of DPPH scavenging and ferric reducing increased and there was a significant difference between the different concentrations studied (P < 0.05). The inhibitory effects of the peptides extracted by the Alcalase were higher than that of Flavourzyme. It can be concluded that a hydrolyzed protein with a molecular weight < 30-KDa can be used as a natural source of antioxidant compounds in the pharmaceutical and food industries.
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References
Abdille M, Singh R, Jayaprakasha G, Jena B (2005) Antioxidant activity of the extracts from Dilleniaindica fruits. Food Chem 90:891–896. https://doi.org/10.1016/j.foodchem.2004.09.002
Abedin MZ, Karim AA, Latiff AA, Gan CY, Ghazali FC, Barzideh Z, Ferdosh S, Akanda MJH, Zzaman W, Karim MR, Sarker MZI (2014) Biochemical and radical-scavenging properties of sea cucumber (Stichopus vastus) collagen hydrolysates. Nat Prod Res 28(16):1302–1305. https://doi.org/10.1080/14786419.2014.900617
Ahn MR, Kumazawa S, Usui Y, Nakamura J, Matsuka M, Zhu F, Nakayama T (2007) Antioxidant activity and constituents of propolis collected in various areas of China. Food Chem 101(4):1383–1392. https://doi.org/10.1016/j.foodchem.2006.03.045
Alemán A, Giménez B, Pérez-Santin E, Gómez-Guillén MC, Montero P (2011) Contribution of Leu and Hyp residues to antioxidant and ACE-inhibitory activities of peptide sequences isolated from squid gelatin hydrolysate. Food Chem 125:334–341. https://doi.org/10.1016/j.foodchem.2010.08.058
Althunibat OY, Ridzwan BH, Taher M, Daud JM, Ichwan JA, Qaralleh H (2013) Antioxidant and cytotoxic properties of two sea cucumber, Holothuria edulis Lesson and Stichopus horrens Selenka. Acta Biol Hung 64:10–20
AOAC (2005) Official methods of analysis of association of official analytical chemists. AOAC, Washington, DC
Asha KK, Remya Kumari KR, Ashok Kumar K, Chatterjee NS, Anandan R, Mathew S (2016) Sequence determination of an antioxidant peptide obtained by enzymatic hydrolysis of oyster Crassostrea madrasensis (Preston). Int J Pept Res Ther 22:1–13
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37(8):911–917. https://doi.org/10.1139/y59-099
Blois MS (1958) Antioxidants determination by the use of a stable free radical. Nature 4617:1199–1200
Brand-Williams W, Cuvelier ME, Berset CLWT (1995) Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol 28(1):25–30. https://doi.org/10.1016/S0023-6438(95)80008-5
Chabeaud A, Vandanjon L, Bourseau P, Jaouen P, Chaplain-Derouiniot M, Guérard F (2009) Performances of ultrafiltration membranes for fractionating a fish protein hydrolysate: application to the refining of bioactive peptidic fractions. Sep Purif Technol 66(3):463–471. https://doi.org/10.1016/j.seppur.2009.02.012
Chen J, Wang Y, Zhong Q (2012) Purification and characterization of a novel angiotensin-I converting enzyme (ACE) inhibitory peptide derived from enzymatic hydrolysate of grass carp protein. Peptides 33:52–58. https://doi.org/10.1016/j.peptides.2011.11.006
Chi CF, Hu FY, Wang B, Li T, Ding GF (2015) Antioxidant and anticancer peptides from the protein hydrolysate of blood clam (Tegillarca granosa) muscle. J Funct Foods 15:301–313. https://doi.org/10.1016/j.jff.2015.03.045
Diba G, Jamili Sh, Ramezani Fard A (2016) Evaluation of antioxidant properties of sea cucumber (Holothuria parva) in two dry and fresh conditions. Iran J Fish Sci 25(4):77–86
Hawa I, Zulaikah M, Jamalodin M, Zainal Abidin AA, Kaswandi MA, Ridzwan BH (1999) The potential of the coelomic fluid in sea cucumber as an antioxidant. J Malays J Nutr 5:55–59
Heo SJ, Park EJ, Lee KW, Jeon YJ (2005) Antioxidant activities of enzymatic extracts from brown seaweeds. Bioresour Technol 96:1613–1623. https://doi.org/10.1016/j.biortech.2004.07.013
Rajapakse N, Mendis E, Byun HG, Kim SK (2005) Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems. J Nutr Biochem 16:562–569. https://doi.org/10.1016/j.jnutbio.2005.02.005
Ketnawa S, Liceaga AM (2017) Effect of microwave treatments on antioxidant activity and antigenicity of fish frame protein hydrolysates. Food Bioprocess Technol 10(3):582–591
Korhonen H, Pihlanto A (2006) Bioactive peptides: production and functionality. Int Dairy J 16(9):945–960
Layne E (1957) Spectrophotometric and turbidimetric methods for measuring proteins. In: Colowick PS, Kaplan NO (eds) Methods in enzymology. Academic press, New York
Li Y, Yu J (2015) Research progress in structure-activity relationship of bioactive peptides. J Med Food 18:147–156. https://doi.org/10.1089/jmf.2014.0028
Li X, Luo YK, Shen HX, You J (2012) Antioxidant activities and functional properties of grass carp (Ctenopharyngodon idellus) protein hydrolysates. J Sci Food Agric 92:292–298. https://doi.org/10.1002/jsfa.4574
Mamelona J, Pelletier EM, Lalancette KG, Legault J, Karboune S, Kermasha S (2007) Quantification of phenolic and antioxidant capacity of Atlantic seacucumber, Cucumariafrondosa. Food Chem 104:1040–1047. https://doi.org/10.1016/j.foodchem.2007.01.016
Mendis E, Rajapakse N, Byun HG, Kim SK (2005) Investigation of jumbo squid (Dosidicus gigas) skin gelatin peptides for their in vitro antioxidant effects. Life Sci 77:2166–2178. https://doi.org/10.1016/j.lfs.2005.03.016
Meyer KA, Sijtsma FP, Nettleton JA, Steffen LM, Van Horn L, Shikany JM, Gross MD, Mursu J, Traber MG, Jacobs Jr DR (2013) Dietary patterns are associated with plasma F2-isoprostanes in an observational cohort study of adults. Free Radic Biol Med, 57, 201–209. https://doi.org/10.1016/j.freeradbiomed.2012.08.574
Nam KA, You SG, Kim SM (2008) Molecular and physical characteristics of squid (Todarodes pacificus) skin collagens and biological properties of their enzymatic hydrolysates. J Food Sci 73:C249–C255. https://doi.org/10.1111/j.1750-3841.2008.00722.x
Oyaizu M (1986) Studies on products of browning reaction: antioxidative activity of products of browning reaction. Jpn J Nutr 44(6):307–315
Pishevarzad F, Youssef Zadi M, Kamrani E, Moeini Zanjani T, Ali Ahmadi A, Keshavarz M (2014) Antioxidant properties of the extracts of two species of Persian Gulf cucumber Holothuria leucospilota and Holothuria parva. Aquat Ecol J 4(1):29–34 (In persian)
Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 160:1–40. https://doi.org/10.1016/j.cbi.2005.12.009
Wang X, Chen H, Fu X, Li S, Wei J (2017) A novel antioxidant and ACE inhibitory peptide from rice bran protein: Biochemical characterization and molecular docking study. LWT 75:93–99. https://doi.org/10.1016/j.lwt.2016.08.047
Wang B, Li L, Chi CF, Ma JH, Luo HY, Xu YF (2013) Purification and characterisation of a novel antioxidant peptide derived from blue mussel (Mytilus edulis) protein hydrolysate. Food Chem 138:1713–1719. https://doi.org/10.1016/j.foodchem.2012.12.002
Wang J, Wang Y, Tang Q, Wang Y, Chang Y, Zhao Q, Xue C (2010) Antioxidation activities of low-molecular weight Gelatin hydrolyzate isolated from the sea cucumber Stichopus japonicus. J Ocean Univ China 9:94–98
Wollgast J, Anklam E (2000) Review on polyphenols in Theobroma cacao: changes in composition during the manufacture of chocolate and methodology for identification and quantification. Food Res Int 33(6):423–447
Wu HT, Jin WG, Sun SG, Li XS, Duan XH, Li Y, Yang YT, Han JR, Zhu BW (2016) Identification of antioxidant peptides from protein hydrolysates of scallop (Patinopecten yessoensis) female gonads. Eur Food Res Technol 242:713–722
Yaghoubzadeh Z, Peyravii Ghadikolaii F, Kaboosi H, Safari R, Fattahi E (2019) Antioxidant activity and anticancer effect of bioactive peptides from rainbow trout (Oncorhynchus mykiss) skin hydrolysate. Int J Pept Res Ther.https://doi.org/10.1007/s10989-019-09869-5
Yaghoubzadeh Z, Peyravii Ghadikolaii F, Kaboosi H, Safari R, Fattahi E (2019) Antioxidant activity and anticancer effect of bioactive peptides from rainbow trout (Oncorhynchus mykiss) skin hydrolysate. Int J Pept Res Ther 1–8. https://doi.org/10.1007/s10989-019-09869-5
Yen GC, Wu JY (1999) Antioxidant and radical scavenging properties of extracts from Ganoderma tsugae. Food Chem 65(3):375–379. https://doi.org/10.1016/S0308-8146(98)00239-8
Zhang M, Mu TH, Sun MJ (2014) Purification and identification of antioxidant peptides from sweet potato protein hydrolysates by Alcalase. J Funct Foods 7:191–200. https://doi.org/10.1016/j.jff.2014.02.012
Zhao YH, Li BF, Liu ZY, Dong SY, Zhao X, Zeng MY (2007) Antihypertensive effect and purification of an ACE inhibitory peptide from sea cucumber gelatin hydrolysate. Process Biochem 42:1586–1591. https://doi.org/10.1016/j.procbio.2007.08.011
Zhou X, Wang C, Jiang A (2012) Antioxidant peptides isolated from sea cucumber Stichopus Japonicus. Eur Food Res Technol 234:441–447
Zou TB, He TP, Li HB, Tang HW, Xia EQ (2016) The structure-activity relationship of the antioxidant peptides from natural proteins. Molecules 21:72. https://doi.org/10.3390/molecules21010072
Acknowledgements
We appreciate the sincere collaboration of the Microbiology Department, Caspian Sea Ecology Research Center (CSERC), Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO) for assistance in conduct of the study.
Funding
This study was supported by the Caspian Sea Ecology Research Center (CSERC), Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Iran. The funding body had no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.
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Safari, R., Yaghoubzadeh, Z. Antioxidant Activity of Bioactive Peptides Extracted from Sea Cucumber (Holothuria leucospilata). Int J Pept Res Ther 26, 2393–2398 (2020). https://doi.org/10.1007/s10989-020-10031-9
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DOI: https://doi.org/10.1007/s10989-020-10031-9