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A DSC study of paeonol-encapsulated liposomes, comparison the effect of cholesterol and stigmasterol on the thermotropic phase behavior of liposomes

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Abstract

Thermotropic phase behaviors of paeonol-encapsulated liposomes containing stigmasterol or cholesterol have been investigated by differential scanning calorimetry. We compared the thermotropic phase behavior of pure dipalmitoylphosphatidylcholine (DPPC) liposomes, sterol/DPPC liposomes, and paeonol/sterol/DPPC liposomes increasing the ratio of paeonol to sterol from 0 to 1, by analyzing the calorimetric parameters of main phase transition of liposomes including phase transition temperature (onset temperature and peak temperature) and phase transition cooperativity. The results showed that paeonol could incorporate into the hydrophobic region of DPPC, thus, decrease phase transition temperature of DPPC. Though stigmasterol interacts with DPPC less favorably than cholesterol, thermotropic phase behavior of paeonol/cholesterol/DPPC liposomes and that of paeonol/stigmasterol/DPPC liposomes are very similar. A phase separation occurred when the molar ratio of paeonol to sterol reached 1:1 in paeonol-encapsulated liposomes, where a paeonol-rich domain coexisted with a sterol-rich domain. The packing order of acyl chains of DPPC in sterol-rich domain is a little higher than that in paeonol-rich domain.

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Acknowledgements

This project was supported by the Funds of Fundamental Research Funds (2011JYBZZJS-050) and the Innovative Research Team (2011-CXTD-13) of Beijing University of Chinese Medicine.

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Authors and Affiliations

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, No.6, Zhonghuan South Road, Wangjing, Chaoyang District, Beijing, 100102, People’s Republic of China

    Rui-Guang Wu, Yu-Rong Wang, Hong-Wei Zhou, Xiao-Hua Zhang & Jun-Ling Hou

  2. Key Laboratory of Bioorganic Phosphorous Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084, People’s Republic of China

    Fu-Gen Wu

  3. Department of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI, 48109, USA

    Fu-Gen Wu

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  1. Rui-Guang Wu
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Correspondence to Rui-Guang Wu.

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Wu, RG., Wang, YR., Wu, FG. et al. A DSC study of paeonol-encapsulated liposomes, comparison the effect of cholesterol and stigmasterol on the thermotropic phase behavior of liposomes. J Therm Anal Calorim 109, 311–316 (2012). https://doi.org/10.1007/s10973-012-2331-5

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  • DOI: https://doi.org/10.1007/s10973-012-2331-5

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