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Purification and radioiodination of 2, 4 di-tertiary- butyl phenol extracted from Lactococcus lactis subsp. lactis CAU: 3138-GM2 and its application on myeloma cells

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Abstract

The goal of the present study was oriented to extraction of a bioactive compound 2,4-di-tertiary-butyl phenol (2,4-DTBP) from Lactococcus lactis subsp. lactis CAU: 3138-GM2 of goat milk origin (accession no. MF354327.1). The 2,4-DTBP was extracted by methanol: chloroform. Radioiodination of 2,4-DTBP using Na125I and chloramine -T was carried out. Effect of radiolabeled tracer and non-radiolabeled of 2,4-DTBP on the inhibition of growth myeloma cells were evaluated. The bioactivity of 2,4-DTBP was tested against Staphylococcus aureus MRSA ATCC 43300, Listeria monocytogenes ATCC 19116, Pseudomonas aeruginosa MTCC 1934, and Escherichia coli MTCC 1610, Clostridium bifermentans MTCC 11273 and Candida albicans MTCC 183.

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Acknowledgements

Authors acknowledge the immense financially support provided by Hot Labs Center, Atomic Energy Authority, Cairo, Egypt

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Authors and Affiliations

  1. Labelled Compounds Department, Hot Labs Centre, Atomic Energy Authority, Cairo, Egypt

    Kh.M. Sallam, Z. S. Nasr, H. M. El-Shershaby & I. Y. Abd El-Ghany

  2. Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt

    N. N. Abed & N. M. Sidkey

  3. Regional Centre for Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt

    A. S. Abd-Elkareim

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Sallam, K., Nasr, Z.S., El-Shershaby, H.M. et al. Purification and radioiodination of 2, 4 di-tertiary- butyl phenol extracted from Lactococcus lactis subsp. lactis CAU: 3138-GM2 and its application on myeloma cells. J Radioanal Nucl Chem 329, 717–730 (2021). https://doi.org/10.1007/s10967-021-07838-1

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