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Investigating the Function of Three Non-Synonymous SNPs in EGFR Gene: Structural Modelling and Association With Breast Cancer

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Abstract

Non-synonymous single nucleotide polymorphisms (nsSNPs) represent common genomic variations that alter protein sequence and function. Some nsSNPs affecting conserved amino acids have been reported to be associated with cancer susceptibility. Interestingly, Epidermal Growth Factor Receptor (EGFR) is commonly overexpressed and mutated in many cancers. In this study, we investigated the structural effect of three deleterious nsSNPs: rs17337451 (R962G), rs1140476 (R977C) and rs17290699 (H988P) within EGFR using computational tools. The modelled mutant dimers showed less stability than wild type EGFR dimer. Furthermore, we showed the important role of R962 and H988 residues in the EGFR dimer formation. We also report preliminary experimental data for SNP R977C suggesting that the variant C977 might confer greater risk for breast cancer. These results contribute to an improved understanding of the EGFR dimer stability and provide new elements for understanding the relationship between EGFR and cancer.

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Abbreviations

EGFR:

Epidermal growth factor receptor

RTK:

Receptor tyrosine kinase

SNP:

Single nucleotide polymorphism

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Acknowledgments

This work was supported by the Ministry of Higher education, Scientific Research, and Technology, Tunisia.

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Authors and Affiliations

  1. Bioinformatics Unit, Centre of Biotechnology of Sfax, P.O. Box 1177 3018, Sfax, Tunisia

    Mouna Choura, Najla Kharrat, Sami Aifa & Ahmed Rebaï

  2. Ecole Nationale d’ingénieurs de Sfax, Sfax, Tunisia

    Fakher Frikha

Authors
  1. Mouna Choura
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  2. Fakher Frikha
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  3. Najla Kharrat
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  4. Sami Aifa
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  5. Ahmed Rebaï
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Correspondence to Ahmed Rebaï.

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Choura, M., Frikha, F., Kharrat, N. et al. Investigating the Function of Three Non-Synonymous SNPs in EGFR Gene: Structural Modelling and Association With Breast Cancer. Protein J 29, 50–54 (2010). https://doi.org/10.1007/s10930-009-9221-0

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  • DOI: https://doi.org/10.1007/s10930-009-9221-0

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