Abstact
Polar organisms should have mechanisms to survive the extremely cold environment. Four genes encoding cold-shock proteins, which are small, cold-induced bacterial proteins, have been cloned from the Antarctic bacterium Streptomyces sp. AA8321. Since the specific functions of any polar bacterial or Streptomyces cold-shock proteins have not yet been determined, we examined the role of cold-shock protein A from Streptomyces sp. AA8321 (CspASt). Gel filtration chromatography showed that purified CspASt exists as a homodimer under physiological conditions, and gel shift assays showed that it binds to single-stranded, but not double-stranded, DNA. Overexpression of CspASt in Escherichia coli severely impaired the ability of the host cells to form colonies, and the cells developed an elongated morphology. Incorporation of a deoxynucleoside analogue, 5-bromo-2′-deoxyuridine, into newly synthesized DNA was also drastically diminished in CspASt-overexpressing cells. These results suggest that CspASt play a role in inhibition of DNA replication during cold-adaptation.
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Abbreviations
- BrdU:
-
5-bromo-2′-deoxyuridine
- CD:
-
circular dichroism
- Csp:
-
cold shock protein
- CspASt :
-
cold-shock protein A from Streptomyces sp. AA8321
- dNTP:
-
deoxynucleotide triphosphate
- dsDNA:
-
double-stranded DNA
- ssDNA:
-
single-stranded DNA
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Acknowledgments
This work was supported from grant number R01-2006-000-11154-0 from the Basic Research Program of the Korea Science & Engineering Foundation, Grant PM05090 from the Korea Ministry of Maritime Affairs and Fisheries, and grant number FPR05B2-211 of 21C Frontier Functional Proteomics Program from the Korea Ministry of Science and Technology.
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Kim, MJ., Lee, Y.K., Lee, H.K. et al. Characterization of Cold-Shock Protein A of Antarctic Streptomyces sp. AA8321. Protein J 26, 51–59 (2007). https://doi.org/10.1007/s10930-006-9044-1
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DOI: https://doi.org/10.1007/s10930-006-9044-1