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Experimental Exploration of Thermostable Poly (β-Hydroxybutyrates) by Geobacillus kaustophilus Using Box-Behnken Design

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Abstract

The polymers/enzymes produced by the microorganisms isolated from the various thermal sources have stability at different levels and their usage areas can be improved according to this characteristic. In this study, prokaryotic diversity of water sample taken from Çitgöl thermal spring in Kütahya-Simav, has been investigated by cultivation techniques. In the result of this study, Geobacillus thermoparaffinivorans, Geobacillus kaustophilus, Geobacillus sp., Geobacillus thermoleovorans and Thermus thermophilus were isolated from water sample. Then, the scanning studies were performed to choose an effective PHB producer with thermal stability. Designing of experiments, analyzing of experimental results, creating the mathematical models and finding optimization conditions were performed using Box-Behnken experimental design method. PHB yield of G. kaustophilus C4-2 increased to 8.66% PHB under the determined conditions which were found as 35 °C, 3.75 days and 10 g/L sucrose. The validity and practicability of this statistical optimization strategy was confirmed relation between predicted and experimental values. The characterization of obtained PHB was performed through analyses including FTIR, H-NMR, TG/DTA and viscosity. According to the result of TG/DTA, PHB was thermal resistant and could remain 14.3% without decomposition at 600 °C.

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Acknowledgements

The study was supported by Eskisehir Osmangazi University Scientific Research Projects Committee (Project No. BAP 201319021). The authors would like to thank Dr. Okan Zafer Yeşilel and Dr. Yeliz Buruk Sahin at Eskisehir Osmangazi University for FTIR spectra of the biomaterial and statistical analysis, respectively. This study is based partly on the PhD thesis of S. Gedikli who is one of the co-authors. We state that there is no conflict of interests in this study.

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Authors and Affiliations

  1. Department of Biology, Graduate School of Natural and Applied Sciences, Eskisehir Osmangazi University, Eskisehir, Turkey

    Serap Gedikli

  2. Department of Biotechnology and Biosafety, Graduate School of Natural and Applied Sciences, Eskisehir Osmangazi University, Eskisehir, Turkey

    Pınar Aytar Çelik & Ahmet Çabuk

  3. Advanced Technologies Research and Application Center, Hacettepe University, Ankara, Turkey

    Murat Demirbilek

  4. Department of Biology, Faculty of Science, Eskisehir Technical University, Eskişehir, Turkey

    Mehmet Burçin Mutlu

  5. Biochemistry Division, Department of Chemistry, Hacettepe University, Ankara, Turkey

    Emir Baki Denkbaş

  6. Department of Biology, Faculty of Arts and Science, Eskisehir Osmangazi University, 26480, Eskisehir, Turkey

    Ahmet Çabuk

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  1. Serap Gedikli
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  2. Pınar Aytar Çelik
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  3. Murat Demirbilek
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  4. Mehmet Burçin Mutlu
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  5. Emir Baki Denkbaş
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  6. Ahmet Çabuk
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Correspondence to Serap Gedikli.

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Gedikli, S., Çelik, P.A., Demirbilek, M. et al. Experimental Exploration of Thermostable Poly (β-Hydroxybutyrates) by Geobacillus kaustophilus Using Box-Behnken Design. J Polym Environ 27, 245–255 (2019). https://doi.org/10.1007/s10924-018-1335-z

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  • DOI: https://doi.org/10.1007/s10924-018-1335-z

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