Abstract
Zinc oxide (ZnO) nanorods decorated on reduced graphene oxide (RGO) nanocomposite was synthesized by one-pot hydrothermal synthesis. The morphology and the properties of the synthesized RGO–ZnO composites were characterized by XRD, FT-IR, Raman spectroscopy, FE-SEM-EDAX, HR-TEM, UV–Vis spectroscopy, and X-ray photo spectroscopy techniques. The antimicrobial properties of the graphene nanocomposite were examined against four different pathogens by agar well diffusion method, and antioxidant properties of the same were examined by four different free radical scavenging assays. It possessed no toxic effects on HEK293 Human embryonic kidney cell line. The synergistic effects between ZnO nanorods and RGO sheets enhanced the antimicrobial and antioxidant properties of the composite. The zinc ions in the solution dispersed on the RGO sheets enabled the intimate contact with microbes and induced the microbes to death. The results state that the RGO–ZnO nanocomposite exhibited remarkably enhanced antimicrobial efficacy and excellent cytotoxic property. The prepared RGO–ZnO nanocomposite was considered as a potent candidate for antibacterial and antioxidant activity.
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J. Yip, L.W. Liu, K.H. Wong, P.H.M. Leung, C.W.M. Yuen, M.C. Cheung, Investigation of antifungal and antibacterial effects of fabric padded with highly stable selenium nanoparticles. J. Appl. Polym. Sci. 131, 8886–8893 (2014)
A.K. Geim, Graphene: status and prospects. Science 324, 1530–1534 (2009)
C.N.R. Rao, A.K. Sood, K.S. Subrahmanyam, A. Govindaraj, Graphene: the new two-dimensional nanomaterial. Nanomaterials, 48, 7752–7777 (2009)
K.S. Novoselov, A.K. Geim,.S.V. Morozov, D. Jiang, Y. Zhang,.S.V. Dubonos, I.V. Grigorieva, A.A. Firsov, Electric field Effect in atomically thin carbon films. Science 306, 666–669 (2004)
Q. Wu, Z. Xu, Y. Yao, A. Liu, G. Shi, Supercapacitors based on flexible graphene/polyaniline nanofiber composite films. ACS Nano. 4, 1963–1970 (2010)
W. Bao, G. Liu, Z. Zhao, H. Zhang, D. Yan, A. Deshpande, B. LeRoy, C.N. Lau, Lithography-free fabrication of high quality substrate-supported and freestanding graphene devices. Nano Res. 3, 98–102 (2010)
D. Wang, D. Choi, J. Li, Z. Yang, Z. Nie, R. Kou, D. Hu, C. Wang, L. Saraf, V.J. Zhang, I. Aksay, A. Liu, Self-assembled TiO2–graphene hybrid nanostructures for enhanced li-ion insertion. ACS Nano 3, 907–914 (2009)
J.I. Paredes, S. Villar-Rodil, A. Martínez-Alonso, J.M.D. Tascon Graphene oxide dispersions in organic solvents. Langmuir 24, 10560–10564 (2008)
G. Williams, B. Seger, P.V. Kamat, TiO2—graphene nanocomposites. UV-assisted photocatalytic reduction of graphene oxide. ACS Nano 2, 1487–1491 (2008)
C. Xu, X. Wang, L. Yang, Y. Wu, Fabrication of a graphene-cuprous oxide composite. J. Solid State Chem. 182, 2486–2490 (2009)
N.T. Hu, Y.Y. Wang, J. Chai, R.G. Gao, Z. Yang, W.E.S. Kong, Y.F. Zhang, Gas sensor based on p-phenylenediamine reduced graphene oxide. Sens. Actuators B 163, 107–114 (2012)
J.C. Lin, B.R. Huang, T.C. Lin, Hybrid structure of graphene sheets/ ZnO nanorods for enhancing electron field emission properties. Appl. Surf. Sci. 289, 384–387 (2014)
B. Jaleh, A. Jabbari, Evaluation of reduced graphene oxide/ZnO effect on properties of PVDF nanocomposite films. Appl. Surf. Sci. 320, 339–347 (2014)
Q. Xiang, J. Yu, M. Jaroniec, Graphene-based semiconductor photocatalysts. Chem. Soc. Rev. 41, 782–796 (2012)
B. Seger, P.V. Kamat, Electrocatalytically active graphene-platinum nanocomposites. Role of 2-D carbon support in PEM fuel cells. J. Phys. Chem. C 113, 7990–7995 (2009)
M.H. Huang, S. Mao, H. Feick, H.Q. Yan, Y. Wu, H. Kind,.E. Weber, R. Russo, P. Yang, Room-temperature ultraviolet. Nanowire Nano. Sci. 292, 1897–1899 (2001)
J.C. Che, C.T. Tang, Preparation and application of granular ZnO/Al2O3 catalyst for the removal of hazardous trichloroethylene. J. Hazard. Mat. 142, 88–96 (2007)
Z.L. Wang, Functional oxide nanobelts: materials, properties and potential applications is nanosystems and biotechnology. Annu. Rev. Phys. Chem. 55, 159–196 (2004)
J. Sawai, Quantitative evaluation of antibacterial activities of metallic oxide powders (ZnO, MgO and CaO) by conductimetric assay. J. Microbiol. Methods 54, 177–182 (2003)
S.H. Choi, Y.P. Zhang, A. Gopalan, K.P. Lee, H.D. Kang, Preparation of catalytically efficient precious metallic colloids by γ-irradiation and characterization. Colloids Surfaces A 256, 165–170 (2005)
K.R. Raghupathi, R.T. Koodali, A.C. Manna, Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticle. Langmuir 27, 4020–4028 (2011)
A. Azam, A.S. Ahmed, M. Oves, M.S. Khan, S.S. Habib, A. Memic, Antimicrobial activity of metal oxide nanoparticles against gram-positive and gram-negative bacteria: a comparative study. Int. J. Nanomed. 7, 6003–6009 (2012)
H. Ma, P.L. Williams, S.A. Diamond, Ecotoxicity of manufactured ZnO nanoparticles—A review. Environ. Pollut. 172, 76–85 (2013)
L. Zhang, Y. Jiang, Y. Ding, M. Povey, D. York, Investigation into the antibacterial behaviour of suspensions of ZnO nanoparticles (ZnO nanofluids). J. Nanopart. Res. 9, 479–489 (2007)
K.M. Kumar, B.K. Mandal, E.A. Naidu, M. Sinha, K.S. Kumar, P.S. Reddy, Synthesis and characterisation of flower shaped zinc oxide nanostructures and its antimicrobial activity. Spectrochimica Acta A 104, 171–174 (2013)
A. Lipovsky, Y. Nitzan, A. Gedanken, R. Lubart, Antifungal activity of ZnO nanoparticles-the role of ROS mediated cell injury. Nanotechnology 22, 101–105 (2011)
S. Vlad, C. Tanase, D. Macocinschi, C. Ciobanu, T. Balaes, D. Filip, D. Gostin, L.M. Gradinaru, Digest J. Nanomater. Biostruct. 7, 51–58 (2012)
J. You, Y. Zhang, Z. Hu, Bacteria and bacteriophage inactivation by silver and zinc oxide nanoparticles. Colloids Surf. 85, 161–167 (2011)
M. Premanathan, K. Karthikeyan, K. Jeyasubramanian, G. Manivannan, Selective toxicity of ZnO nanoparticles toward Gram-positive bacteria and cancer cells by apoptosis through lipid peroxidation. Nanotechnol. Biol. Med. 7, 184–192 (2012)
J. Sawai, T. Yoshikawa, Quantitative evaluation of antifungal activity of metallic oxide powders (MgO, CaO and ZnO) by an indirect conductimetric assay. J. Appl. Microbiol. 96, (803–809) (2004)
P.K. Stoimenov, R.L. Klinger, G.L. Marchin, K.J. Klabunde, Metal oxide nanoparticles as bactericidal agents. Langmuir 18, 6679–6686 (2002)
R. Brayner, R. Ferrari-Iliou, N. Brivois, S. Djediat, M. Benedetti, F. Fievet, Toxicological impact studies based on Escherichia coli bacteria in ultrafine ZnO nanoparticles colloidal medium. Nano Lett. 6, 866–870 (2006)
Z. Huang, Xu.. Zheng,.D. Yan, G. Yin,. X. Liao, Y. Kang, Y. Yao,. D. Huang, B. Hao, Toxicological effect of ZnO nanoparticles based on bacteria. Langmuir 24, 4140–4144 (2008)
A. Kahru, H.C. Dubourguier, I. Blinova, A. Ivask, K. Kasemets, Biotests and biosensors for ecotoxicology of metal oxide nanoparticles: a minireview. Sensors 8, 5153–5170 (2008)
O. Akhavan, E. Ghaderi, Toxicity of graphene and graphene oxide nanowalls against bacteria. ACS Nano 4, 5731–5736 (2010)
H Wenbing, P. Cheng, L. Weijie, H. Qing, F. Chunhai, L. Min, L. Xiaoming, Graphene-based antibacterial paper. Acs Nano 4, 4317–4323 (2010)
Y. Chang, S.T. Yang, J.H. Liu, E. Dong, Y.W. Wang, A. Cao, Y. Liu, H. Wang, In vitro toxicity evaluation of graphene oxide on A549 cells, Toxicol. Lett. 200, 201–210 (2011)
A. Lukowiak, bA. Kedziora, W. Strek, Antimicrobial graphene family materials: progress, advances, hopes and fears. Adv. Colloid Interface Sci. 236, 101–112 (2016)
N.W. Pu, C.A. Wang, Y. Sung, Y.M. Liu, M.D. Ger, Production of few-layer graphene by supercritical CO2 exfoliation of graphite. Mater. Lett. 63, 1987–1989 (2009)
C.Y. Kong, W.L. Song, M.J. Meziani, K.N. Tackett, L. Cao, A.J. Farr, A. Anderson, Y.P. Sun, Supercritical fluid conversion of graphene oxides. J. Supercrit. Fluids 61, 206–211 (2012)
S. Bykkam, S. Narsingam, M. Ahmadipour, T. Dayakar, K. Venkateswara Rao, C. Shilpa Chakra, S. Kalakotla, Few layered graphene Sheet decorated by ZnO Nanoparticles for Anti-Bacterial. Appl. Superlattices Microstruct. 83, 776–784 (2015)
A.R. Chowdhuri, S. Tripathy, S. Chandra, S. Roy, S.K. Sahu, A ZnO decorated chitosan-graphene oxide nanocomposite shows significantly enhanced antimicrobial activity with ros generation. RSC Adv. 5, 49420–49428 (2015)
C.H. Chen, Y.F. Dai, Effect of chitosan on interfacial polymerization of aniline. Carbohydr. Polym. 84, 840–843 (2011)
W.S. Hummers, R.E. Offeman, Preparation of graphitic oxide. J. Am. Chem. Soc. 80, 1339 (1958)
K. Karthikeyan, R. Mohan, S.J. Kim, Graphene oxide as a photocatalytic material. Appl. Phys. Lett. 98, 244101–244103 (2011)
A.V. Badarinath, K.M. Rao, C.M.S. Chetty, V. Ramkanth, T.V.S. Rajan, K. Gnanaprakash, A review on in-vitro antioxidant methods: comparisions, correlations and considerations. Int. J. PharmTech Res. 2, 1276–1285 (2010)
D. Menaga, S. Rajakumar, P.M. Ayyasamy, Free radical scavenging activity of methanolic extract of pleurotus florida mushroom. Int. J. Pharm. Pharm. Sci. 5, 0975–1491 (2013)
R.J. Ruch, S.J. Cheng, E. Klaunig, Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis. 10, 1003–1008 (1989)
G. Kaur, V. Gupta, P. Bansal, Innate antioxidant activity of some traditional formulations. J. Adv. Pharm. Technol. Res. 8, 39–42 (2017)
C. Sousa, P. Valentao, F.R. Ferreres, M. Seabra, P.B. Andrade, Tronchuda Cabbage (Brassica oleracea L. var. costata DC): Scavenger of reactive nitrogen species. J. Agric. Food. Chem. 56, 4205–4211 (2008)
L. Zhang, G. Du, B. Zhou, L. Wang, Green synthesis of flower-like ZnO decorated reduced graphene oxide composites. Ceram. Int. 40, 1241–1244 (2014)
X. Qin, Y. Zhang, N. Xue, P. Kittiwattanothai, N. Kongsittikul, N. Rodthongkum, S. Limpanart, M. Ma, L. Riping, A facile synthesis of nanorods of ZnO/graphene oxide composites with enhanced photocatalytic activity. Appl. Surf. Sci. 321, 226–232 (2014)
B. Jiang, C. Tian, Q. Pan, Z. Jiang, J.Q. Wang, W. Yan, H. Fu, Enhanced photocatalytic activity and electron transfer mechanisms of graphene/TiO2 with exposed {001} facets. J. Phys. Chem. C 115, 23718–23725 (2011)
A. Mihranyan, A.P. Llagostera, R. Karmhag, M. Strømme, Moisture sorption by cellulose powders of varying crystallinity. Int. J. Pharm. 269, 433–442 (2004)
K. Zhou, Y. Zhu, X. Yang, X. Jiang, C. Li, Preparation of graphene–TiO2 composites with enhanced photocatalytic activity. New J. Chem. 35, 353–359 (2011)
Y. Zhang, C. Pan, TiO /graphene composite from thermal reaction of graphene oxide and its photocatalytic activity in visible light. J. Mater. Sci. 46, 2622–2626 (2011)
J. Shen, M. Shi, B. Yan, H. Ma, N. Li, M. Ye, Ionic liquid-assisted one-step hydrothermal synthesis of TiO2-reduced graphene oxide composites. Nano Res. 4, 795–806 (2011)
A. Kaschner, U. Haboeck, M. Strassburg, M. Strassburg, G. Kaczmarczyk, A. Hoffmann, C. Thomsen, A. Zeuner, H.R. Alves, D.M. Hofmann, B.K. Meyer, Appl. Phys. Lett. 80, 1909–1911 (2002)
O. Yamamoto, J. Sawai, T. Sasamoto, Change in antibacterial characteristics with doping amount of ZnO in MgO–ZnO solid solution. J. Inorg. Mater. 2, 451–454 (2000)
L. Zhang, Y. Ding, M. Povey, D. York, ZnO nanofluids—A potential antibacterial agent. Prog. Nat. Sci. 18, 939–944 (2008)
R. Hariharan, S. Senthilkumar, A. Suganthi, M. Rajarajan, Synthesis and characterization of doxorubicin modified ZnO/PEG nanomaterials and its photodynamic action. J. Photochem. Photobiol B 116, 56–65 (2012)
O. Yamamoto, M. Hotta, J. Sawai, T. Sasamoto, H. Kojima, Antifungal characteristics of spherical carbon materials with zinc oxide. J. Ceram. Soc. Jpn. 111, 8, 614–616 (2003)
P. Dibrov, J. Dzioba, K.K. Gosink, C.C. Hase, Chemiosmotic mechanism of antimicrobial activity of Ag + in Vibrio cholera. Chemotherapy 46, 2668–2670 (2002)
G.H. Naik, K.I. Priyadarsini, J.G. Satav, M.M. Banavalikar, D.P. Sohoni, M.K. Biyani, H. Mohan, Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine. Phytochemistry. 63, 97–104 (2003)
J. Pal, S. Ganguly, K.S. Tahsin, K. Acharya, In vitro free radical scavenging activity of wild edible mushroom, Pleurotus squarrosulus (Mont.) Singer. Indian J. Exp. Biol. 47, 1210–1218 (2010)
J.P. Saikia, S. Paul, B.K. Konwar, S.K. Samdarshi, Nickel oxide nanoparticles: a novel antioxidant. Colloids Surf. B 78, 146–148 (2010)
J. Wang, X.Z. Yuan, Y. Jin, H. Tian, Song, Free radical and reactive oxygen species scavenging activities of peanut skins extract. Food. Chem. 104, 242–250 (2007)
A. Kumaran, R.J. Karunakaran, In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT 40, 344–352 (2007)
J. Sawai, R. Doi, Y. Maekawa, T. Yoshikawa, H. Kojima, Indirect conductimetric assay of antibacterial activities. J. Ind. Microbiol. Biotechnol. 29, 296–298 (2002)
Y.N. Chang, M. Zhang, L. Xia, J. Zhang, G. Xing, The toxic effects and mechanisms of CuO and ZnO. Nanoparticles Mater. 5, 2850–2871 (2012)
S.H. Hu, Y.W. Chen, W.T. Hung, I.W. Chen, S.-Y. Chen, Quantum-dot-tagged reduced graphene oxide nanocomposites for bright fluorescence bioimaging and photothermal therapy monitored in situ. Adv. Mater. 24, 1748–1754 (2012)
H. Chen, M.B. Muller, K.J. Gilmore, G.G. Wallace, D. Li, Mechanically Strong, electrically conductive, and biocompatible graphene paper. Adv. Mater. 20, 3557–3561 (2008)
H. Hong, J. Shi, Y. Yang, Y. Zhang, J.W. Engle, R.J. Nickles, X. Wang, W. Cai, Cancer-targeted optical imaging with fluorescent zinc oxide nanowires. Nano Lett. 11, 3744–3750 (2011)
Acknowledgements
The authors express their gratitude to the Professor and Head, Department of Industrial Chemistry for enabling the HR-SEM analysis and the School of Physics, Alagappa University, Karaikudi, Tamil Nadu, India for the provision of XRD, and Raman analysis.
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Rajeswari, R., Prabu, H.G. Synthesis Characterization, Antimicrobial, Antioxidant, and Cytotoxic Activities of ZnO Nanorods on Reduced Graphene Oxide. J Inorg Organomet Polym 28, 679–693 (2018). https://doi.org/10.1007/s10904-017-0711-9
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DOI: https://doi.org/10.1007/s10904-017-0711-9