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A Novel DNBS-based Fluorescent Probe for the Detection of H2S in Cells and on Test Strips

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Abstract

Hydrogen sulfide (H2S) plays a key role in the physiology and pathology of organisms, and H2S in the environment is easily absorbed and harmful to health. It is of great significance to develop a probe with good selectivity, high sensitivity and good stability that can detect hydrogen sulfide inside and outside organisms. In this work, we designed a novel “turn-on” fluorescent probe CIM-SDB for the detection of H2S. The probe CIM-SDB used indene-carbazole as the fluorophore and 2,4-dinitrobenzenesulfonyl as the recognition site. The probe CIM-SDB exhibited high selectivity and sensitivity to H2S (detection limit as low as 123 nM). Moreover, the probe CIM-SDB was successfully applied to the detection of intracellular exogenous and endogenous H2S, and the test strips prepared by the probe CIM-SDB could realize the convenient and rapid detection of H2S.

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Funding

This work was financially supported by the Anhui Province University Natural Science Research Project (No. KJ2020A0014), the Anhui Provincial Natural Science Foundation (No. 1808085ME155), and the National Training Programs of Innovation and Entrepreneurship for Undergraduates (No. S202310357037).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Anhui University, Hefei, 230601, PR China

    Wenxiang Wang, Yudie Gan, Huaqin Jiang, Min Fang, Zhenyu Wu & Weiju Zhu

  2. Anhui Province Key Laboratory of Environment-friendly Polymer Materials, Anhui University, Hefei, 230601, PR China

    Min Fang

  3. Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Anhui University, Hefei, 230601, PR China

    Weiju Zhu

  4. School of Materials Science and Engineering, Anhui University, Hefei, 230601, PR China

    Cun Li

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  1. Wenxiang Wang
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  3. Huaqin Jiang
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  4. Min Fang
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Contributions

W.W. performed the synthesis, optical property test and the first draft of the manuscript. Y.G. carried out the synthesis of probe. H.J. carried out the bioimaging. Z.W., W.Z. and C.L. analyzed the data and revised the manuscript. M.F. gave guidance ideas and experimental platform. All authors read and approved the final manuscript.

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Correspondence to Min Fang.

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Wang, W., Gan, Y., Jiang, H. et al. A Novel DNBS-based Fluorescent Probe for the Detection of H2S in Cells and on Test Strips. J Fluoresc (2024). https://doi.org/10.1007/s10895-024-03660-6

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