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Preliminary Insights into the Fluorescence and Oxidative Characteristics of Flavin - DNA Systems on PVP - Coated Silver Nanoparticles

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Abstract

Emissive features of flavins (Riboflavin/RF, Flavin MonoNucleotide/FMN and Flavin Adenine Dinucleotide/FAD) labeled native Deoxyribonucleic Acid (DNA) on Polyvinylpyrrolidone (PVP)-coated silver nanoparticles (SNPs), have been studied. The dual emission of flavins in DNA−PVP-coated SNPs systems is strongly influenced by the reaction time and temperature. Changes in the RF emissive features occur as a side effect when DNA is covalently linked hence, the RF destruction depends on DNA damage. Even if in an oxidation process, the FAD-DNA – PVP-coated SNPs system acts as a weak scavenger of reactive oxygen species, its antioxidant activity is approx. five times higher than that of RF-DNA−PVP-coated SNPs system. Destruction of RF by a riboflavin-mediated DNA photo-oxidation process that occurs on PVP-coated SNPs is suggested. Results have relevance in the redox process of riboflavin and provide valuable information for the further development of novel flavin-based SNPs systems as fluorescent antioxidant markers to solve several biological barriers in humans, such as protein-DNA interaction, cell binding.

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Acknowledgements

This study was carried out within the research direction “Flavins – based BioNanoSystems Probed by Luminescence Methods” in the frame of the “Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy.

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  1. Institute of Physical Chemistry “Ilie Murgulescu” of the Romanian Academy, Splaiul Independentei 202, Bucharest, 060021, Romania

    Mariana Voicescu

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  1. Mariana Voicescu
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Voicescu, M. Preliminary Insights into the Fluorescence and Oxidative Characteristics of Flavin - DNA Systems on PVP - Coated Silver Nanoparticles. J Fluoresc (2024). https://doi.org/10.1007/s10895-023-03549-w

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