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Development and Application of a Multiplex Fluorescent PCR for Shigella Detection and Species Identification

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Abstract

This study was to develop a multiplex fluorescent PCR for Shigella detection and species identification. Five primer pairs for Shigella detection and species identification were designed by Primer Premier 5.0. The multiplex fluorescent PCR was optimized by varying single parameter while other parameters were maintained. The multiplex fluorescent PCR assay could correctly detect Shigella and identify four Shigella species with a detection limits of 10 pg genomic DNA per reaction. Testing different strains and clinical samples confirmed the sensitivity and specificity of the multiplex fluorescent PCR. The newly developed multiplex fluorescent PCR assay is simple, sensitive and specific for Shigella detection and species identification. It has a potential to be used in routine Shigella detection and species identification in clinical laboratories.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

Code Availability

Not applicable.

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Acknowledgements

We are grateful to Zhejiang and Jiashan Center for Disease Control and Prevention, P. R. China, for providing reference strains. This study was supported by Science and Technology Program of Jiaxing City (No. 2017AY33071).

Funding

This study was supported by Science and Technology Program of Jiaxing City (No. 2017AY33071) Science and Technology Program of Jiaxing City, 2017AY33071, Henghui Wang.

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Authors and Affiliations

  1. Jiaxing Center for Disease Control and Prevention, No. 486, Wen Qiao Road, Zhejiang, Jiaxing, 314050, China

    Peiyan He, Henghui Wang, Yong Yan, Guoying Zhu & Zhongwen Chen

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  1. Peiyan He
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  2. Henghui Wang
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  3. Yong Yan
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  4. Guoying Zhu
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  5. Zhongwen Chen
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Contributions

PH and ZC designed research, GZ, HW and YY provided strains, PH analyzed data and wrote the paper with the input from other authors.

Corresponding author

Correspondence to Zhongwen Chen.

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He, P., Wang, H., Yan, Y. et al. Development and Application of a Multiplex Fluorescent PCR for Shigella Detection and Species Identification. J Fluoresc 32, 707–713 (2022). https://doi.org/10.1007/s10895-021-02876-0

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  • DOI: https://doi.org/10.1007/s10895-021-02876-0

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