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Study of the Binding between Camptothecin Analogs and FTO by Spectroscopy and Molecular Docking

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Abstract

In this work, the interaction between camptothecin (CPT) analogs and fat mass and obesity associated (FTO) was investigated using spectroscopy and molecular docking. From the experimental results, it was found that the CPT analogs caused the fluorescence quenching of FTO through a static quenching procedure. The binding constants and thermodynamic parameters at three different temperatures, the number of binding sites were obtained, which suggested that the hydrophobic interaction and electrostatic force played major role in the reaction between CPT analogs and FTO. Results revealed that 10-hydroxycamptothecin was the strongest quencher.

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Acknowledgements

We are grateful to the National Natural Science Foundation of China (No. 81330075), and 2015 Key science and technology plan project of Henan province (152102310065) for financial support.

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Authors and Affiliations

  1. College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou, 450001, China

    Ting Ren, Zechun Wang, Lijiao Zhang, Ning Wang, Xinxin Han, Ruiyong Wang & Junbiao Chang

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  1. Ting Ren
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  2. Zechun Wang
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  3. Lijiao Zhang
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  4. Ning Wang
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  5. Xinxin Han
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Correspondence to Ruiyong Wang or Junbiao Chang.

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Ren, T., Wang, Z., Zhang, L. et al. Study of the Binding between Camptothecin Analogs and FTO by Spectroscopy and Molecular Docking. J Fluoresc 27, 1467–1477 (2017). https://doi.org/10.1007/s10895-017-2086-2

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  • DOI: https://doi.org/10.1007/s10895-017-2086-2

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