Skip to main content

Advertisement

SpringerLink
Log in

FRET Sensor for Erythrosine Dye Based on Organic Nanoparticles: Application to Analysis of Food Stuff

  • ORIGINAL ARTICLE
  • Published:
Journal of Fluorescence Aims and scope Submit manuscript

Abstract

An aqueous suspension of fluorescent nanoparticles (PHNNPs) of naphthol based fluorescent organic compound 1-[(Z)-(2-phenylhydrazinylidene) methyl] naphthalene −2-ol (PHN) were prepared using reprecipitation method shows bathochromically shifted aggregation induced enhanced emission (AIEE) in the spectral region where erythrosine (ETS) food dye absorbs strongly. The average size of 72.6 nm of aqueous suspension of PHNNPs obtained by Dynamic light scattering results shows a narrow particle size distribution. The negative zeta potential of nano probe (−22.6 mV) responsible to adsorb oppositely charged analyte on its surface and further permit to bind nano probe and analyte within the close distance proximity required for efficient fluorescence resonance energy transfer (FRET) to take place from donor (PHNNPs) to acceptor (ETS). Systematic FRET experiments performed by measuring fluorescence quenching of PHNNPs with successive addition of ETS solution exploited the use of the PHNNPs as a novel nano probe for the detection of ETS in aqueous solution with extremely lower limit of detection equal to 3.6 nM (3.1 ng/mL). The estimation of photo kinetic and thermodynamic parameters such as quenching rate constant, enthalpy change (∆H), Gibbs free energy change (∆G) and entropy change (∆S) was obtained by the quenching results obtained at different constant temperatures which were found to fit the well-known Stern–Volmer relation. The mechanism of binding and fluorescence quenching of PHNNPs by ETS food dye is proposed on the basis of results obtained in photophysical studies, thermodynamic parameter, energy transfer efficiency, critical energy transfer distance (R0) and distance of approach between donor–acceptor molecules (r). The proposed FRET method based on fluorescence quenching of PHNNPs was successfully applied to develop an analytical method for estimation of ETS from food stuffs without interference of other complex ingredients.

A fluorescent organic nanoprobe developed for the detection of erythrosine (ETS) food dye in aqueous medium based on fluorescence resonance energy transfer (FRET). The FRET process between donor (nanoparticles) and acceptor (ETS dye) arises due to oppositely charge attraction through hydrophobic interactions. The proposed method was successfully applied to quantitative determination of ETS dye in food stuff sample collected from local market.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12
Scheme 2
Fig. 13

Similar content being viewed by others

References

  1. Peska V, Jahn M, Stolcova L, Schulz V, Proska J, Prochazka M, Weber K, Cialla-May D, Popp J (2015) Quantitative SERS analysis of Azorubine (E 122) in sweet drinks. Anal Chem 87:2840–2844

    Article  Google Scholar 

  2. Vinas P, Pardo-Martianez M, Pez-Garciaa I, Hernandezcordoba M (2002) Rapid determination of mercury in food colorants using Electrothermal atomic absorption spectrometry with slurry sample introduction. J Agric Food Chem 50:949–954

    Article  CAS  PubMed  Google Scholar 

  3. Sharma V, McKone H, Markow P (2011) A global perspective on the history, use, and identification of synthetic food dyes. J Chem Educ 88:24–28

    Article  CAS  Google Scholar 

  4. Guo J, Wu H, Du L, Fu Y (2013) Determination of brilliant blue FCF in food and cosmetic samples by ionic liquid independent disperse liquid–liquid micro-extraction. Anal Methods 5:4021–4026

    Article  CAS  Google Scholar 

  5. Ramakrishnan SP, Bagya Lakshmi L, Surya PR (2011) Estimation of synthetic dye erythrosine in food stuff and formulation and effect of dye on the protein binding of drug in BSA. Der Pharmacia Lettre 3:361–373

    CAS  Google Scholar 

  6. http://articles.mercola.com/sites/articles/archive/2011/02/24/are-you-or-your-family-eating-toxic-food-dyes.aspx

  7. http://www.diseaseproof.com/archives/cat-food-safety.html

  8. Kaur A, Gupta U (2014) Preconcentration of erythrosine dye using β-cyclodextrin epichlorohydrin polymer as a solid phase extractant. World J Pharm Pharm Sci 4:812–819

    Google Scholar 

  9. Bhopate DP, Mahajan PG, Garadkar KM, Kolekar GB, Patil SR (2015) Polyvinyl pyrrolidone capped fluorescent anthracene nanoparticles for sensing fluorescein sodium in aqueous solution and analytical application for ophthalmic samples. Luminesce. doi:10.1002/bio.2858

    Google Scholar 

  10. Ordoudi SA, Tsimidou MZ (2011) Consideration of fluorescence properties for the direct determination of erythrosine in saffron in the presence of other synthetic dyes. Food Addit Contam 28:417–422

    Article  CAS  Google Scholar 

  11. Yuan L, Lin W, Zheng K, Zhu S (2013) FRET-based small-molecule fluorescent probes: rational design and Bioimaging applications. Acc Chem Res 6:1462–1473

    Article  Google Scholar 

  12. Zhang H, Liu R, Tan Y, Haowei Xie W, Lei H, Cheung H, Sun H (2015) A FRET - based ratiometric fluorescent probe for Nitroxyl detection in living cells. ACS Appl Mater Interfaces 7:5438–5443

    Article  CAS  PubMed  Google Scholar 

  13. Bhattar SL, Kolekar GB, Patil SR (2011) FRET between anthracene and Proflavine Hemisulphate in micellar solution and analytical application on determination of Proflavine Hemisulphate. J Dispers Sci Technol 32:23–27

    Article  CAS  Google Scholar 

  14. Bhattar SL, Kolekar GB, Patil SR (2008) Fluorescence resonance energy transfer between perylene and riboflavin in micellar solution and analytical application on determination of vitamin B2. J Lumin 128:306–310

    Article  CAS  Google Scholar 

  15. Saha J, Datta Roy A, Dey D, Chakraborty S, Bhattacharjee D, Paul PK, Hussain SA (2015) Investigation of fluorescence resonance energy transfer between fluorescein and rhodamine 6G. Spectrochim Acta A Mol Biomol Spectrosc 149:143–149

    Article  CAS  PubMed  Google Scholar 

  16. Dalavi DK, Kamble AA, Bhopate DP, Mahajan PG, Kolekar GB, Patil SR (2015) TNPs as a novel fluorescent sensor for the selective recognition of fast green FCF: a spectrofluorimetric approach. RSC Adv 5:69371–69377

    Article  CAS  Google Scholar 

  17. Dige NC, Pore DM (2015) Green aspect for multicomponent synthesis of spiro[4H-indeno[1,2-b]pyridine-4,3′-[3H]indoles]. Syn comm 45: 2498–2510

  18. Tunç T, Tezcan H, Saglam S, Dilek N (2014) Comparative experimental and theoretical studies ofN-(4-Methylbenzylidene)-N0-(2-carboxyphenyl) hydrazine novel Schiff base. Spectrochim Acta Part A: Mol Biomol Spectro 127:490–497

    Article  Google Scholar 

  19. Das DK, Goswami P, Sarma P (2013) A new highly selective fluorescence lead (II) probe. J Fluoresc 23:503–508

    Article  CAS  PubMed  Google Scholar 

  20. Ghosh S, Alam A, Gangulya A, Guchhait N (2015) A new highly selective fluorescence lead (II) probe. Spectrochim Acta Part A: Mol Biomol Spectro 149:869–874

    Article  CAS  Google Scholar 

  21. Mahajan PG, Desai NK, Dalavi DK, Bhopate DP, Kolekar GB, Patil SR (2015) Cetyltrimethylammonium bromide capped 9-Anthraldehyde nanoparticles for selective recognition of phosphate anion in aqueous solution based on fluorescence quenching and application for analysis of chloroquine. J Fluoresc 25:31–38

    Article  CAS  PubMed  Google Scholar 

  22. Wang F, Han M, Yi Mya K, Wang Y, Lai Y (2005) Aggregation driven growth of size-tunable organic nanoparticles using electronically altered conjugated polymers. J Am Chem Soc 127:10350–10355

    Article  CAS  PubMed  Google Scholar 

  23. Foster T, Sinanoglu O (1996) Quantum chemistry, vol 3. Academic Press, New York, p. 93

    Google Scholar 

  24. Lakowicz JR (2006) Principles of fluorescence spectroscopy, 3rd edn. Plenum Press, New York

    Book  Google Scholar 

  25. Mahajan PG, Bhopate DP, Kamble AA, Dalavi DK, Kolekar GB, Patil SR (2015) Selective sensing of Fe2+ ions in aqueous solution based on fluorescence quenching of SDS capped rubrene nanoparticles: application in pharmaceutical formulation. Anal Methods 7:7889–7898

    Article  CAS  Google Scholar 

  26. Forster T (1959) 10th Spiers memorial lecture. Transfer mechanisms of electronic excitation. Discuss Faraday Soc 27:7–17

    Article  Google Scholar 

  27. Chen G, Song F, Xiong X, Peng P (2013) Fluorescent nanosensors based on fluorescence resonance energy transfer (FRET). Ind Eng Chem Res 52:11228–11245

    Article  CAS  Google Scholar 

  28. Ross PD, Subramanian S (1981) Thermodynamics of protein association reactions: forces contributing to stability. Biochemistry 20:3096–3102

    Article  CAS  PubMed  Google Scholar 

  29. Hu Y, Liu Y, Zhao RM, Dong JX, Qu SS (2006) Spectroscopic studies on the interaction between methylene blue and bovine serum albumin. Photochem Photobiol A 179:324–329

    Article  CAS  Google Scholar 

  30. Jiang XY, Li WX, Cao H (2008) Study of the interaction between trans-resveratrol and BSA by the multi-spectroscopic method. J Solut Chem 37:1609–1623

    Article  CAS  Google Scholar 

  31. Wangoo N, Kaushal J, Bhasin KK, Mehta SK, Suri CR (2010) Zeta potential based colorimetric immunoassay for the direct detection of diabetic marker HbA1c using gold nanoprobes. Chem Commun 46:5755–5757

    Article  CAS  Google Scholar 

  32. Teng Y, Jia X, Li J, Wang E (2015) Ratiometric fluorescence detection of Tyrosinase activity and dopamine using thiolate-protected gold nanoclusters. Anal Chem 87:4897–4902

    Article  CAS  PubMed  Google Scholar 

  33. Zhang K, Yu T, Liu F, Sun M, Yu H, Liu B, Zhang Z, Jiang H, Wang S (2014) Selective fluorescence turn-on and ratiometric detection of organophosphate using dual-emitting Mn-doped ZnS nanocrystal probe. Anal Chem 86:11727–11733

    Article  CAS  PubMed  Google Scholar 

  34. Albers AE, Okreglak VS, Chang CJ (2006) A FRET-based approach to ratiometric fluorescence detection of hydrogen peroxide. J Am Chem Soc 128:9640–9641

    Article  CAS  PubMed  Google Scholar 

  35. Mahajan PG, Bhopate DP, Kolekar GB, Patil SR (2015) N-methyl isatin nanoparticles as a novel probe for selective detection of Cd2+ ion in aqueous medium based on chelation enhanced fluorescence and application to environmental sample. Sensors Actuators B 220:864–872

    Article  CAS  Google Scholar 

  36. Trandafir I, Nour V, Ionica M (2009) The liquid chromatographic quantification of some synthetic colorants in soft drinks. Sci Stu Res 1:73–82

    Google Scholar 

  37. Minioti KS, Sakellariou CF, Thomaidis NS (2007) Determination of 13 synthetic food colorants in water-soluble foods by reversed-phase high-performance liquid chromatography coupled with diode-array detector. Anal Chim Acta 583:103–110

    Article  CAS  PubMed  Google Scholar 

  38. Kaur A, Gupta U (2012) Simultaneous spectrophotometric Determiantion of eosin and erythrosine with Cr (VI) reagent in micellar media using mean centering of ratio spectra. Chem Sci Trans 1:424–430

    Article  Google Scholar 

  39. Kaur A, Gupta U (2012) Simultaneous spectrophotometric determination of eosin and erythrosine in pharmaceutical and food samples by using mean centering of ratio spectra method. Int J Res Chem Environ 2:55–62

    Google Scholar 

Download references

Acknowledgments

One of the authors PGM is grateful to the University Grants Commission (UGC), New Delhi for providing financial assistance in the form of UGC-BSR-SAP fellowship (F.25-1/2013-14(BSR)/7-183/2007(BSR)- May 2014). We are also grateful to the Department of Science and Technology (DST), New Delhi for providing funds under FIST-Level-II program for infrastructure improvement and University Grants Commission (UGC), New Delhi for financial support through DRS - Phase- II program to the Department of Chemistry, Shivaji University, Kolhapur.

Author information

Authors and Affiliations

  1. Fluorescence Spectroscopy Research Laboratory, Department of Chemistry, Shivaji University, Kolhapur-, Maharashtra, 416004, India

    Prasad G. Mahajan, Dhanaji P. Bhopate, Govind B. Kolekar & Shivajirao R. Patil

Authors
  1. Prasad G. Mahajan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dhanaji P. Bhopate
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Govind B. Kolekar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shivajirao R. Patil
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shivajirao R. Patil.

Electronic supplementary material

ESM 1

(DOC 901 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mahajan, P.G., Bhopate, D.P., Kolekar, G.B. et al. FRET Sensor for Erythrosine Dye Based on Organic Nanoparticles: Application to Analysis of Food Stuff. J Fluoresc 26, 1467–1478 (2016). https://doi.org/10.1007/s10895-016-1839-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10895-016-1839-7

Keywords

Search

Navigation