Abstract
In recent years, sustainable green chemistry sets eco-efficiency approaches to synthesis next generation nanomaterials using plant sources as reducing agents. Among them, reduced Graphene oxide (rGO) has been developing as biocompatible nanomaterials in biomedicine. The present work deals with facile synthesis of rGO using leaf extract of Lantana camara (LC). The bioreduction of graphene oxide (GO) to reduced Graphene oxide by L. camara (LCrGO) was confirmed by spectroscopic techniques such as UV–visible, XRD, FTIR, Raman spectrometer, Zeta potential and DLS. SEM and TEM microscopic revealed the presence of thin nano sheets of LCrGO. EDX detected intense signals of Carbon (86%) and Oxygen (13%) present in LCrGO. The bioreduced LCrGO showed potential in vitro biological effects. DPPH assay showed 79% radical inhibition at 500 µg/ml. The LCrGO exhibited enhanced bactericidal effects against B. subtilis (16 mm) and E. coli (12 mm) at 500 µg/ml. MTT assay showed concentration dependent cytotoxic effects against lung cancer cell lines (A549) with decrease in cell viability (44%) at maximum concentration of LCrGO (500 µg/ml). From the observations, it is concluded that rGO nanosheets synthesized using L. camara could be used for formulation of nanomedicine.
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D. K. Gupta, R. S. Rajaura, and K. Sharma (2015). Int. J. Environ. Sci. Technol. 1, 16–24.
J. C. Pieretti, W. R. Rolim, F. F. Ferreira, C. B. Lombello, and A. B. Seabra (2019). J. Clust. Sci.. https://doi.org/10.1007/s10876-019-01670-0.
R. Mahendran, D. Sridharan, K. Santhakumar, T. A. Selvakumar, P. Rajasekar, and J. Jang (2016). Indian J. Mater. Sci. 1–10.
T. Y. Suman, S. R. Radhika Rajasree, R. Ramkumar, C. Rajthilak, and P. Perumal (2014). Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 118, 11–16.
A. Cadete, A. Olivera, M. Besev, P. K. Dhal, L. Gonçalves, A. J. Almeida, G. Bastiat, J. P. Benoit, M. D. L. Fuente, M. G. Fuentes, and D. Torres (2019). Sci. Rep. 9, (11555–11566), 2019.
G. Prabhavathi, A. Mariam, and M. Karunanithy (2019). Int. J. Bio-Pharma Res. 8, 2486–2490.
A. N. A. Khatua, E. Priyadarshini, P. Rajamani, A. Patel, J. Kumar, M. R. S. Muthupandian, H. Barabadi, A. Prasad, L. Ghosh, and B. Paul (2019). J. Clust. Sci.. https://doi.org/10.1007/s10876-019-01624-6.
S. O. Ogunyemi, Y. Abdallah, M. Zhang, H. Fouad, X. Hong, E. Ibrahim, Md M I Masum, A. Hossain, J. Mo, and B. Li (2019). Artif. Cells Nanomed. Biotechnol. 47, 341–352.
L. Feng and Z. Liu (2011). Graphene in biomedicine: opportunities and challenges. Nanomedicine 6, (2), 317–324.
A. P. Nikam, M. P. Ratnaparkhiand, and P. C. Shilpa (2014). J. Drug Deliv. Ther. 3, 1121–1127.
M. Shah, D. Fawcett, S. Sharma, and S. K. Tripathy (2015) Green Synthesis of Metallic Nanoparticles via Biological Entities.
P. Chamoli, R. Sharma, M. K. Das, and K. K. Kar (2016). RSC Adv. 6, 96355–96366.
S. Maria Coro, P. Florina, M. Lidia, C. Socaci, and S. Pruneanu (2019). Mater. Sci.. https://doi.org/10.1007/s11706-019-0452-5.
S. Priyadarsini, S. Mohanty, S. Mukherjee, S. Basu, and M. Mishra (2018). J. Nano struct Chem. 8, 123–137.
G. Lee and B. S. Kim (2014). Biotechnol. Prog. 30, 463–469.
J. Wen, B. K. Salunke, and B. S. Kim (2017). J. Chem. Technol. Biotechnol. 92, 1428–1435.
B. Chandu, V. S. S. Mosali, B. Mullamuri, and H. B. Bollikolla (2017). Carbon Lett. 21, 74–80.
C. Li, Z. Zhuang, X. Jin, and Z. Chen (2017). Appl. Surf. Sci. 422, 469–474.
X. Zhu, X. Xu, F. Liu, J. Jin, L. Liu, Y. Zhi, Z. W. Chen, Z. S. Zhou, and J. Yu (2017). Nanomater. Nanotechnol. 7, 1–7.
Y. Wang, Z. X. Shi, and J. Yin (2011). ACS Appl. Mater. Interfaces. 3, 1127–1133.
C. K. Chua and M. Pumera (2014). Chem Soc Rev. 43, 291–312.
G. Bhattacharya, S. Sas, S. Wadhwa, A. Mathur, J. Mclaughlin, and S. S. Roy (2017). RSC Adv. 7, 26680–26688.
S. Babu, B. Kumar, R. Vankayala, and P. Kalluru (2015). Spectrochim. Act. Part A Mol. Biomol. Spectrosc. 145, 117–124.
C. Singh, A. Ali, and G. Sumana (2016). ACS Sustain. Chem. Eng. 4, 1–34.
G. Amala, J. Saravanan, D. J. Yoo, A. R. Kimc, and G. G. Kumar (2017). New J. Chem. https://doi.org/10.1039/x0xx00000x.
C. Namasivayam, N. Muniasamy, K. Gayatri, M. Rani, and K. Ranganathan (1996). Bioresour. Technol. 57, 37–43.
S. B. Maddinedi and B. K. Mandal (2016). Curr. Nanosci. 12, 94–102.
F. Tavakoli, M. Salavati-niasari, and F. Mohandes (2015). Green synthesis and characterization of graphene nanosheets. Mater. Res. Bull. 63, 51–57.
R. K. Upadhyay, N. Soin, S. Saha, A. Barman, and S. S. Roy (2015). Mater. Lett. 160, 355–358.
T. Kuila, S. Bose, P. Khanra, and A. Kumar (2012). Carbon. 50, 914–921.
C. Karthikeyan, K. Ramachandran, S. Sheet, D. J. Yoo, Y. S. Lee, Y. S. Kumar, A. R. Kim, and G. G. Kumar (2017). ACS Sustain. Chem. Eng. 5, 4897–4905.
F. Lorestani, Z. Shahnavaz, P. Mn, Y. Alias, and N. S. A. Manan (2015). Sens. Actuat. B Chem. 208, 389–398.
M. Vinothkannan, C. Karthikeyan, G. Gnana Kumar, A. R. Kim, and D. J. Yoo (2015). Spectrochim. Acta - Part A Mol. Biomol. Spectrosc. 136, 256–264.
K. V. B. R. Sanjeeb Kalita, G. Kumar, and L. Karthik (2012). Adv. Pharmacol. Sci. 5, (711–715), 2012.
Khare CP, Springer, (2007). https://www.springer.com/gp/book/9780387706375.
K. K. R. Kirtikar, B. D. Basu, and I. C. S Kirtikar (1975). Ranchoddas, 1849–1917.
W. S. Jr. Hummers and R. E. Offema (1957). Am. Chem. Soc. 80, 1339.
B. Paulchamy, G. Arthi, and L. Bd (2015). Nanomed. Nanotechnol. 6, 1–4.
B. Adhikari, S. K. Dhungana, M. Waqas Ali, A. Adhikari, I. D. Kim, and D. H. Shin (2019). J. Saudi Soc. Agric. Sci. 18, 437–442.
S. K. Bopp and T. Lettieri (2008). BMC Pharmacol. 8, 1–11.
K. Krishnamoorthy, R. Mohan, and S. J. Kim (2011). Appl. Phys. Lett. 98, 244101.
R. Naz and A. Bano (2013). Asian Pac. J. Trop. Dis. 3, 480–486.
S. N. Alam, N. Sharma, and L. Kumar (2017). Graphene 6, 1–18.
A. A. Moosa, and J. N. Jaafar (2017). 7, 38–47.
P. M. J. Firdhouse and Lalitha (2014). Int. Nano Lett. 4, 103–108.
S. Mahata, A. Sahu, P. Shukla, A. Rai, M. Singh, and V. K. Rai (2018). New J. Chem. 42, 19945–19952.
M. Fathy, R. H. Mohamed, and K. Amany (2019). Graphene Technol. 4, 33–40.
E. B. L. A. Noel, J. Faucheu, J. M. Chenal, and J. P. Viricelle (2014). Polymer. 55, 5140–5145.
N. M. S. Hidayah, W. W. Liu, C. W. Lai, N. Z. Noriman, C. S. Khe, U. Hashim, and H. C. Lee (2017). AIP Conf. Proc. 1892, 150002.
A. C. Ferrari (2007). Solid State Commun. 143, 47–57.
M. Abdolahad, M. Janmaleki, S. Mohajerzadeh, O. Akhavan, and S. Abbasi (2013). Mater. Sci. Eng. 33, 1498–1505.
A. Ani, A. Lina, W. A. Yehye, F. A. Kadir, N. M. Hashim, M. A. Alsaadi, N. M. Julkapli, and V. K. S. Hsiao (2019). Plos ONE 50, 1–24.
S. Yaragalla, R. Rajendran, J. Jose, M. A. Almaadeed, N. Kalarikkal, and S. Thomas (2016). Mater. Sci. Eng. 65, 345–353.
N. I. Zaaba, K. L. Foo, U. Hashim, S. J. Tan, W. Liu, and C. H. Voon (2017). Procedia Eng. 184, 469–477.
K. Muthoosamy, R. G. Bai, I. B. Abubakar, S. M. Sudheer, H. N. Lim, H. S. Loh, N. M. Huang, C. H. Chi, and S. Manickam (2015). Int. J. Nanomed. 10, 1505–1519.
W. Zhang, W. He, and X. Jing (2010). J. Phys. Chem. 114, 10368–10373.
D. Suresh, H. Nagabhushana, and S. C. Sharma (2014). Mater. Lett. 10–12.
F. D. J. Qiu, L. Chen, Q. Zhu, D. Wang, W. Wang, X. Sun, and X. Liu (2012). Food Chem. 135, 2366–2371.
D. Bhakta and D. Ganjewala (2009). J. Sci. Res. 1, 363–369.
M. K. Swamy, U. R. Sinniah, and M. S. Akhtar (2015). Evid. Based Complement. Altern. Med. 9, 1–10.
S. Liu, T. H. Zeng, M. Hofmann, E. Burcombe, J. Wei, and R. Jiang (2011). ACS Nano 5, (9), 6971–6980.
H. N. Lim, N. M. Huang, and C. H. Loo (2012). J. Non. Cryst. Solids. 358, 525–530.
P. N. Khanam and A. Hasan (2019). Int. J. Biol. Macromol. 126, 151–158.
L. Zhang, J. Xia, Q. Zhao, L. Liu, and Z. Zhang (2010). Small. 6, 537–544.
E. O. Sousa, C. M. B. A. Miranda, C. B. Nobre, A. A. Boligon, M. L. Athayde, and J. G. M. Costa (2015). Ind. Crops Prod. 70, 7–15.
K. Lingaraju, H. Raja Naika, G. Nagaraju, and H. Nagabhushana (2019). Biotechnol. Rep. 24, 00376.
Y. Chang, S. T. Yang, J. H. Liu, E. Dong, Y. Wang, A. Cao, Y. Liu, and H. Wang (2011). Toxicol. Lett. 200, 201–210.
J. Chen, H. Liu, C. Zhao, G. Qin, G. Xi, T. Li, X. Wang, and T. Chen (2014). Biomaterials 35, 4986–4995.
V. Nandakumar, T. Singh, and S. K. Katiyar (2008). Cancer Lett. 269, 378–387.
S. B. Mahato, N. P. Sahu, S. K. Roy, and O. P. Sharma (1994). Tetrahedron 50, 9439–9446.
J. Kaur, M. Sharma, P. D. Sharma, and M. P. Bansal (2010). Am. J. Biomed. Sci. 2, 79–90.
Y. Ma, H. Shen, X. Tu, and Z. Zhang (2014). Nanomedicine. 9, 1565–1580.
J. L. Wilding and W. F. Bodmer (2014). Cancer Res. 74, 2377–2384.
T. A. Tabish, A. Tanveer, M. Z. I. Pranjol, H. Hayat, A. A. M. Rahat, T. M. Abdullah, J. L. Whatmore, and S. Zhang (2017). Nanotechnology 28, 1–16.
Acknowledgements
Authors are highly thankful to Dr. K. Sathiyanarayanan, Professor and Dr. A. Thanigaivelan, National Post-Doctoral Researcher at Department of Chemistry, Vellore Institute of Technology (VIT), Vellore-632014 for their support to carry out this research work. The author also conveys special thanks to Mr. Anooj ES, Director & Research Consultant, Xcellogen Biotech Pvt Ltd., Nagercoil, Tamil Nadu, India.
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Thiyagarajulu, N., Arumugam, S. Green Synthesis of Reduced Graphene oxide Nanosheets Using Leaf Extract of Lantana camara and Its In-Vitro Biological Activities. J Clust Sci 32, 559–568 (2021). https://doi.org/10.1007/s10876-020-01814-7
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DOI: https://doi.org/10.1007/s10876-020-01814-7