Abstract
Tissue engineering is a multidisciplinary field of research in which the cells, biomaterials, and processes can be optimized to develop a tissue substitute. Three-dimensional (3D) architectural features from electrospun scaffolds, such as porosity, tortuosity, fiber diameter, pore size, and interconnectivity have a great impact on cell behavior. Regarding tissue development in vitro, culture conditions such as pH, osmolality, temperature, nutrient, and metabolite concentrations dictate cell viability inside the constructs. The effect of different electrospun scaffold properties, bioreactor designs, mesenchymal stem cell culture parameters, and seeding techniques on cell behavior can be studied individually or combined with phenomenological modeling techniques. This work reviews the main culture and scaffold factors that affect tissue development in vitro regarding the culture of cells inside 3D matrices. The mathematical modeling of the relationship between these factors and cell behavior inside 3D constructs has also been critically reviewed, focusing on mesenchymal stem cell culture in electrospun scaffolds.
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The authors wish to thank the Stem Cell Research Institute, the Coordination for the Improvement of Higher Level Personnel (CAPES), and the Study and Project Financer (FINEP) for financial support.
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Paim, Á., Tessaro, I.C., Cardozo, N.S.M. et al. Mesenchymal stem cell cultivation in electrospun scaffolds: mechanistic modeling for tissue engineering. J Biol Phys 44, 245–271 (2018). https://doi.org/10.1007/s10867-018-9482-y
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DOI: https://doi.org/10.1007/s10867-018-9482-y