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Biocompatibility of highly macroporous ceramic scaffolds: cell adhesion and morphology studies

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Abstract

Hydroxyapatite porous scaffolds can be used for tissue engineering applications since they can serve as templates for cell adhesion, proliferation and ultimately for tissue repair. One way to address this issue is to evaluate the cell adhesion using several characterization techniques namely, cytotoxicity assays and cell visualization. On the other hand, when using highly macroporous scaffolds some techniques may not be adequate for evaluation, such as MTT. In this work, cytotoxicity assay (MTS), scanning electron microscopy (SEM) and Confocal laser scanning microscopy (CLSM) were used to evaluate cell adhesion in highly macroporous hydroxyapatite scaffolds. It was possible to observe that some techniques were not suitable to evaluate cell adhesion. In addition, it was shown that for this kind of scaffolds, confocal laser scanning microscopy is a powerful tool for cell adhesion and proliferation evaluation.

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Authors and Affiliations

  1. Laboratório de Biomateriais, INEB – Instituto de Engenharia Biomédica, Rua do Campo Alegre 823, Porto, 4150-180, Portugal

    Sandra Teixeira, Maria Pia Ferraz & Fernando J. Monteiro

  2. Departamento de Engenharia Metalúrgica e Materiais, Faculdade de Engenharia, Universidade do Porto, Porto, Portugal

    Sandra Teixeira & Fernando J. Monteiro

  3. Faculdade de Ciências da Saúde da Universidade Fernando Pessoa, Rua Carlos da Maia, 296, Porto, 4200-150, Portugal

    Maria Pia Ferraz

Authors
  1. Sandra Teixeira
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  2. Maria Pia Ferraz
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  3. Fernando J. Monteiro
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Correspondence to Sandra Teixeira.

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Teixeira, S., Ferraz, M.P. & Monteiro, F.J. Biocompatibility of highly macroporous ceramic scaffolds: cell adhesion and morphology studies. J Mater Sci: Mater Med 19, 855–859 (2008). https://doi.org/10.1007/s10856-007-3005-x

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  • DOI: https://doi.org/10.1007/s10856-007-3005-x

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