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Redox-responsive biodegradable nanogels for photodynamic therapy using Chlorin e6

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Abstract

Recent advances in drug carrier design in the field of photodynamic therapy (PDT) have stimulated the development of numerous sophisticated drug delivery carriers. We developed novel biodegradable and biocompatible nanogels as PDT carriers. The nanogels were synthesized by ATRP technique using inverse miniemulsion and their biodegradability was determined in the presence of glutathione. The model photosensitizer (PS) was encapsulated in the biodegradable nanogels by simple mixing and sonication. The cellular uptake of the PS-loaded nanogels and the cytotoxicity of the nanogels before and after laser irradiation were determined using HeLa cells. The results showed that the Ce6-nanogel complex was well internalized into the tumor cells, and the Ce6-loaded nanogels did not influence on cellular viability of the cells before light irradiation. Under light exposure, however, the Ce6-nanogel complex-treated HeLa cells revealed strong photoactivity. These nanogels may enhance therapeutic efficacy of PSs without any complex chemical modifications with PSs.

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Acknowledgements

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2013R1A2A2A01068818); the National Research Foundation of Korea NRF—2014R1A1A2008659; and the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2013R1A1A4A010101185). The authors also acknowledge the Korea Basic Science Institute for assistance with the FT-NMR spectrometer.

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Authors and Affiliations

  1. Department of Polymer Science and Engineering, Pusan National University, Busan, 609-735, Korea

    Hyoyong Kim, Byoungjae Kim, Chaeyeon Lee, Eun-Ju Ha & Hyun-jong Paik

  2. Center of Photodynamic Therapy (PDT), Diatech Korea Co., Ltd, Songpa-gu, Seoul, 138-826, Korea

    Jung Lim Ryu & Seong-Jin Hong

  3. Department of Bio and Chemical Engineering, Hongik University, Sejong, 339-701, Korea

    Jinku Kim

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  1. Hyoyong Kim
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Correspondence to Eun-Ju Ha or Hyun-jong Paik.

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Kim, H., Kim, B., Lee, C. et al. Redox-responsive biodegradable nanogels for photodynamic therapy using Chlorin e6. J Mater Sci 51, 8442–8451 (2016). https://doi.org/10.1007/s10853-016-0104-4

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  • DOI: https://doi.org/10.1007/s10853-016-0104-4

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