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Electrochemical sensor for determination of aging state at single cell level under different pressures of cell capturing

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Abstract

Although aging is a strong and independent predictor of cardiovascular disease, an effective method to evaluate the degree of aging has yet to be reported. Here, we present a straightforward approach to determine aging in a rapid and quantitative manner, using a microfluidic-based electrochemical sensor equipped with a flexible membrane actuator. The flexible membrane in the sensor captures single endothelial cells of various ages (3, 4, and 18 month-old) under different cell capturing pressures (250, 300, and 350 kPa). The sensing electrodes in the sensor measure electrochemical impedance spectra of each cell group in terms of resistance and reactance. An optimal condition showing significant differences in resistance and reactance between different age groups was experimentally determined at a frequency of 1 MHz and a pressure of 350 kPa (p < 0.001).

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Acknowledgements

This research was supported by Chonnam National University (Smart Plant Reliability Center) grant funded by the Ministry of Education, South Korea, (2020R1A6C101B197). All correspondence should be addressed to the authors Jung-Joon Cha and Hyeon Woo Kim.

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Authors and Affiliations

  1. Department of Mechanical Design Engineering, Chonnam National University, 50 Daehak-ro, Yosu, Jeonnam, 59626, South Korea

    Yangkyu Park

  2. Division of Cardiology, Cardiovascular Center, Korea University Anam Hospital, Korea University of College of Medicine, 73 Goryeodae-ro, Seongbuk-gu, Seoul, 02841, South Korea

    Jung-Joon Cha

  3. Department of Urology, Pusan National University Hospital, 179 Gudeok-ro, Seo-gu, Busan, 49241, South Korea

    Hyeon Woo Kim

  4. Department of Urology, Biomedical Research Institute, Pusan National University Hospital, 179 Gudeok-ro, Seo-gu, Busan, 49241, South Korea

    Hyeon Woo Kim

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  1. Yangkyu Park
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  2. Jung-Joon Cha
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Correspondence to Jung-Joon Cha or Hyeon Woo Kim.

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“Dedicated to the memory of Jacques Vicens.”

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Park, Y., Cha, JJ. & Kim, H.W. Electrochemical sensor for determination of aging state at single cell level under different pressures of cell capturing. J Incl Phenom Macrocycl Chem 101, 313–320 (2021). https://doi.org/10.1007/s10847-021-01054-w

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  • DOI: https://doi.org/10.1007/s10847-021-01054-w

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