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Selection of human blastocysts with a high implantation potential based on timely compaction

  • Embryo Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Recently, we established a noninvasive system for selecting human blastocysts with a high pre-transfer implantation potential based on first and second division patterns. The present study was carried out to improve the selection system.

Methods

Embryos that completed first and second divisions within 25.90 and 37.88 h after culture, respectively, were selected using a time-lapse incubator. We examined the effects of compaction and blastocyst formation times on pregnancy rates after transferring these embryos at the blastocyst stage.

Results

The completion of compaction and blastocyst formation times (79.93 and 97.47 h after culture, respectively) of embryos resulting in pregnancies after transfer were significantly (P < 0.01) shorter than those (86.46 and 100.34 h after culture, respectively) of embryos that failed to induce pregnancies. Embryo selection based on completion of compaction time improved pregnancy rates (40.9 vs. 74.6%, P < 0.01).

Conclusions

Of the embryos that formed two cells during the first division within 25.90 h after culture and four cells during the second division within 37.88 h after culture, those that completed compaction within 79.93 h after culture before reaching the blastocyst stage had a high implantation potential.

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Acknowledgements

The authors thank their fellow embryologist’s doctors and nursing staff at Aiiku Ladies Clinic (Kagoshima, Japan) for their technical assistance and support.

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Authors and Affiliations

  1. Aiiku Ladies Clinic, 1-40-2 Komatsubara, Kagoshima, 891-0114, Japan

    Yamato Mizobe, Yuji Ezono, Makoto Tokunaga, Naoto Oya, Reiko Iwakiri, Naomi Yoshida, Yumi Sato & Nanase Onoue

  2. The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan

    Yamato Mizobe & Kazuchika Miyoshi

Authors
  1. Yamato Mizobe
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  2. Yuji Ezono
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  3. Makoto Tokunaga
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  4. Naoto Oya
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  5. Reiko Iwakiri
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  6. Naomi Yoshida
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  7. Yumi Sato
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  8. Nanase Onoue
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  9. Kazuchika Miyoshi
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Corresponding author

Correspondence to Yamato Mizobe.

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Funding declaration

The studied received no external funding.

Conflicts of interest

The authors declare that they have no conflict of interest.

Research involving human participants

This study was approved by the Institutional Review Board of AIIKUKAI Medical Corporation.

Informed consent

Written informed consent for their treatment and for their outcomes to be described was obtained from all patients.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Institutional and National Research Committee and the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Ethical considerations

All study participants provided informed consent, and the study design was approved by the appropriate ethics review boards. Written informed consent for their treatment and outcomes to be described was obtained from all patients.

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Mizobe, Y., Ezono, Y., Tokunaga, M. et al. Selection of human blastocysts with a high implantation potential based on timely compaction. J Assist Reprod Genet 34, 991–997 (2017). https://doi.org/10.1007/s10815-017-0962-y

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  • DOI: https://doi.org/10.1007/s10815-017-0962-y

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