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Detection of aneuploidy rate for chromosomes X, Y and 8 by fluorescence in-situ hybridization in spermatozoa from patients with severe non-obstructive oligozoospermia

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To evaluate the frequency of sperm nuclei disomy for chromosomes 8, X, and Y in patients with severe non-obstructive oligozoospermia and to assess possible correlations between sperm nuclei aneuploidy and semen parameters or a particular clinical phenotype.

Materials and methods

The sperm aneuploidy rate for chromosomes X, Y, and 8 were assessed in 16 infertile men with severe non-obstructive oligozoospermia and 7 healthy men with normal semen parameters. The frequency of sperm aneuploidy was compared between several patients groups according to their clinical and biological factors.

Results

The total rate of chromosomally abnormal spermatozoa was significantly higher in patients with severe oligozoospermia compared to control group (P < 0.001). A significant relationship was found between the age of patients, sperm concentration, and morphology and the mean rate of sex chromosomes disomy. In addition to the low sperm count (<5 × 106/ml), an elevated FSH level and an exposed to an elevated temperature are two major predictive factors leading to the production of higher numbers of chromosomally abnormal gametes.

Conclusion

Patients with severe oligozoospermia, who are potential candidates for assisted reproduction technology, presented a high level of sex numerical chromosome abnormalities, and consequently are at high risk of chromosome abnormalities in their offspring.

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Authors and Affiliations

  1. Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, Sousse, 4000, Tunisia

    Soumaya Mougou-Zerelli, Sonia Brahem, Molka Kammoun, Hatem Elghezal, Mounir Ajina & Ali Saad

  2. Medical Office of Gynaecology and Obstetrics, Avenue M. Karoui, Sousse, 4000, Tunisia

    Mehdi Jerbi

Authors
  1. Soumaya Mougou-Zerelli
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  2. Sonia Brahem
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  3. Molka Kammoun
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  4. Mehdi Jerbi
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  5. Hatem Elghezal
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  6. Mounir Ajina
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  7. Ali Saad
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Corresponding author

Correspondence to Sonia Brahem.

Additional information

Soumaya Mougou-Zerelli and Sonia Brahem equally contribute to the work.

Capsule

Patients with severe oligozoospermia presented a high level of sex numerical chromosome abnormalities, and consequently are at high risk of chromosome abnormalities in their offspring.

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Mougou-Zerelli, S., Brahem, S., Kammoun, M. et al. Detection of aneuploidy rate for chromosomes X, Y and 8 by fluorescence in-situ hybridization in spermatozoa from patients with severe non-obstructive oligozoospermia. J Assist Reprod Genet 28, 971–977 (2011). https://doi.org/10.1007/s10815-011-9621-x

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  • DOI: https://doi.org/10.1007/s10815-011-9621-x

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