Abstract
This work aimed to improve the techniques for cultivating the species Gelidium floridanum, testing different sources of calcium. Tetraspores and explants were grown in the laboratory for 20 days with seawater enriched with 50% von Stosch solution and oyster or mussel shell powder (252 mg L-1 and 336 mg L-1), or calcium chloride (147 mg L-1 and 295 mg L-1). Tetraspores or explants cultivated with no calcium salts or shell powder were used as control. Tetraspore germination rate and morphology, and germling morphology, average length, and growth rate were evaluated. Besides, the morphology, upright axes formation and growth rate of explants were also evaluated. There was no difference in tetraspore germination among treatments and control. Treatments with shell powder impaired tetraspore viability, indicated by a greenish color, cellular disorganization, and delay in germ tube development. Germlings cultivated with CaCl2 show better initial development but with no improvement compared to control. Concerning explants, those cultivated with mussel shell powder showed the highest growth rate (3.92 and 4.28% day-1, according to concentration) in relation to other treatments. In particular, those cultivated with 252 mg L-1 mussel shell powder had a significant formation of upright axes (10.15 ± 0.85 upright axes) compared to the control (5.18 ± 0.42 upright axes). Based on these results, we recommend using mussel shell powder at a concentration of 252 mg L-1 to optimize explant cultivation.
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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgments
CPB thanks Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina (FAPESC) for the master's scholarship. LH thanks Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the Productivity Fellowship (Process number 311680/2020-8). This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001. We also thanks Fernando Zwierzikowski da Silva for the technical support and Prof. Zenilda L. Bouzon for the Plant Cell Biology Laboratory infrastructure.
Funding
This work was funding by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001, Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina (FAPESC) for the master's scholarship of Camila Pereira Bruzinga and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the Productivity Fellowship (Process number 311680/2020-8) of Leila Hayashi.
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Bruzinga, C.P., Simioni, C. & Hayashi, L. Shell powder as source of calcium in the development of Gelidium floridanum (Rhodophyta, Gelidiales) tetraspores and explants. J Appl Phycol 34, 2579–2588 (2022). https://doi.org/10.1007/s10811-022-02796-x
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DOI: https://doi.org/10.1007/s10811-022-02796-x