Skip to main content
SpringerLink
Log in

Green extraction of polysaccharides from Arthrospira platensis using high pressure homogenization

  • Published:
Journal of Applied Phycology Aims and scope Submit manuscript

Abstract

The efficiency of high pressure homogenization as cell disruption method was investigated on the cyanobacterium Arthrospira platensis for the recovery of polysaccharides following the concept of green chemistry, mainly solvent and energy saving. The highest yield of polysaccharides (15.1 ± 0.8 g (100 g)−1 dry weight) was obtained at a solid concentration of 14.5% (w/w), inlet pressure of 500 bar and after 4 homogenization cycles. High-pressure treatment resulted in a 2.5-fold higher yield than the conventional hot water method. Purified polysaccharides extracted under the optimized conditions were isolated and characterized by ATR-FTIR and HPSEC. It was shown that polysaccharides of A. platensis can be extracted using high pressure homogenization under mild conditions (short time, ambient temperature, no chemicals, neutral pH) with a high yield of recovery and a specific energy consumption of 0.41 kWh \( {\mathrm{kg}}_{\mathrm{DW}}^{-1}, \)which is close to the minimum energy demand recommended for an extraction step within biorefinery processes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Belay A, Ota Y, Miyakawa K, Shimamatsu H (1993) Current knowledge on potential health benefits of Spirulina. J Appl Phycol 5:235–241

    Google Scholar 

  • Carullo D, Aberaa BD, Casazzab A, Donsìa F, Peregob P, Ferraria G, Pataro G (2018) Effect of pulsed electric fields and high pressure homogenization on the aqueous extraction of intracellular compounds from the microalgae Chlorella vulgaris. Algal Res 31:60–69

    Google Scholar 

  • Chaiklahan R, Chirasuwan N, Triratana P, Loha V, Tia S, Bunnag B (2013) Polysaccharide extraction from Spirulina sp. and its antioxidant capacity. Int J Biol Macromol 58:73–78

    CAS  PubMed  Google Scholar 

  • Chaiklahana R, Chirasuwana N, Lohab V, Bunnag B (2008) Lipid and fatty acids extraction from the cyanobacterium Spirulina. ScienceAsia 34:299–305

    Google Scholar 

  • Chen H, Yang T, Chen M, Chang Y, Lin C, Wang EI, Ho C, Huang K, Yu C, Yang F, Wu S, Lu Y, Chao LK (2012) Application of power plant flue gas in a photobioreactor to grow Spirulina algae and a bioactivity analysis of the algal water-soluble polysaccharides. Bioresour Technol 120:256–263

    CAS  PubMed  Google Scholar 

  • Chirasuwan N, Chaiklahan R, Ruengjitchatchawalya M, Bunnag B, Tanticharoen M (2007) Anti HSV-1 activity of Spirulina platensis polysaccharide. Kasetsart J (Nat Sci) 41:311–318

    CAS  Google Scholar 

  • Chisti Y, Moo-Young M (1986) Disruption of microbial cells for intracellular products. Enzym Microb Technol 8:194–204

    CAS  Google Scholar 

  • Choi WY, Lee HY (2016) Effective production of bioenergy from marine Chlorella sp. by high-pressure homogenization. Biotechnol Biotechnol Equip 30:81–89

    CAS  Google Scholar 

  • Cohen Z (1997) The chemical of Spirulina. In: Vonshak A (ed) Spirulina platensis (Arthrospira): physiology, cell-biology and biotechnology. Taylor & Francis Inc., Philadelphia, pp 175–204

    Google Scholar 

  • Costa JAV, Freitas BCB, Rosa GM, Moraes L, Morais MG, Mitchel G (2019) Operational and economic aspects of Spirulina-based biorefinery. Bioresour Technol 292:121946

    PubMed  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    CAS  Google Scholar 

  • El-Baky H, El-Baz KF, El-Latife SA (2013) Induction of sulfated polysaccharides in Spirulina platensis as response to nitrogen concentration and its biological evaluation. J Aquault Res Dev 5:1–8

    Google Scholar 

  • Fakhfakh N, Abdelhedi O, Jdir J, Nasri M, Zouari N (2017) Isolation of polysaccharides from Malva aegyptiaca and evaluation of their antioxidant and antibacterial properties. Int J Biol Macromol 105:1519–1525

    CAS  PubMed  Google Scholar 

  • Floury J, Bellettre J, Legrand J, Desrumaux A (2004) Analysis of a new type of high pressure homogeniser. A study of the flow pattern. Chem Eng Sci 59:843–853

    CAS  Google Scholar 

  • Garcia-Ortega X, Reyes C, Montesinos JL, Valero F (2015) Overall key performance indicator to optimizing operation of high-pressure homogenizers for a reliable quantification of intracellular components in Pichia pastoris. Front Biotechnol Bioeng 3:1–9

    Google Scholar 

  • Grimi N, Dubois A, Marchal L, Jubeau S, Lebovka NI, Vorobiev E (2014) Selective extraction from microalgae Nannochloropsis sp. using different methods of cell disruption. Bioresour Technol 153:254–259

    CAS  PubMed  Google Scholar 

  • Günerken E, D'Hondt E, Eppink MHM, Garcia-Gonzalez L, Elst K, Wijffels RH (2015) Cell disruption for microalgae biorefineries. Biotechnol Adv 33:243–260

    PubMed  Google Scholar 

  • Halim R, Harun R, Danquah MK, Webley PA (2012) Microalgal cell disruption for biofuel development. Appl Energy 91:116–121

    CAS  Google Scholar 

  • Harrison R, Todd P, Rudge S, Petrides D (2003) Cell lysis and flocculation. In: Harrison R (ed) Bioseparations science and engineering. Oxford University Press, London, pp 83–93

    Google Scholar 

  • He J, Xu Y, Chen H, Sun P (2016) Extraction, structural characterization, and potential antioxidant activity of the polysaccharides from four seaweeds. Int J Mol Sci 17:1–17

    Google Scholar 

  • Illman AM, Scragg AH, Shales SW (2000) Increase in Chlorella strains calorific values when grown in low nitrogen medium. Enzym Microb Technol 27:631–635

    CAS  Google Scholar 

  • Jensen S, Petersen BO, Omarsdottir S, Paulsen BS, Duus JO, Olafsdottir ES (2013) Structural characterisation of a complex heteroglycan from the cyanobacterium Nostoc commune. Carbohydr Polym 91:370–376

    CAS  PubMed  Google Scholar 

  • Kim J, Yoo G, Lee H, Lim J, Kim K, Kim CW, Park MS, Yang JW (2013) Methods of downstream processing for the production of biodiesel from microalgae. Biotechnol Adv 31:862–887

    CAS  PubMed  Google Scholar 

  • Kim DY, Vijayan D, Praveenkumar R, Han JI, Lee K, Park JY, Chang WS, Lee JS, Oh YK (2016) Cell-wall disruption and lipid/astaxanthin extraction from microalgae: Chlorella and Haematococcus. Bioresour Technol 199:300–310

    CAS  PubMed  Google Scholar 

  • Kleinig AR, Middelberg APJ (1998) On the mechanism of microbial cell disruption in high-pressure homogenisation. Chem Eng Sci 53:891–898

    CAS  Google Scholar 

  • Kurd F, Samavati V (2015) Water soluble polysaccharides from Spirulina platensis: extraction and in vitro anti-cancer activity. Int J Biol Macromol 74:498–506

    CAS  PubMed  Google Scholar 

  • Lee AK, Lewis DM, Ashman PJ (2012) Disruption of microalgal cells for the extraction of lipids for biofuels: processes and specific energy requirements. Biomass Bioenergy 46:89–101

    CAS  Google Scholar 

  • Majdoub H, Mansour BM, Chaubet FS, Roudesli M, Maaroufi MR (2009) Anticoagulant activity of a sulfated polysaccharide from the green alga Arthrospira platensis. Biochim Biophys Acta 1790:1377–1381

    CAS  PubMed  Google Scholar 

  • Middelberg APJ (2000) Microbial cell disruption by high pressure homogenization. In: Dessai MA (ed) Downstream processing of proteins: methods and protocols. Humana Press, London, pp 11–21

    Google Scholar 

  • Norton T, Sun DW (2008) Recent advances in the use of high pressure as an effective processing technique in the food industry. Food Bioprocess Technol 1:2–34

    Google Scholar 

  • Pugh N, Ross SA, ElSohly HN, ElSohly MA, Pasco DS (2001) Isolation of three high molecular weight polysaccharide preparations with potent immunostimulatory activity from Spirulina platensis, Aphanizomenon flos-aquae and Chlorella pyrenoidosa. Planta Med 67:737–742

  • Rajasekar P, Palanisamy S, Manoharan R, Vinosha M, Elakkiya M, Marudhupandi T, You SG (2019) Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance. Int J Biol Macromol 141:809–821

    CAS  PubMed  Google Scholar 

  • Safi C, Frances C, Ursu AV, Laroche C, Pouzet C, Vaca-Garcia C, Pontalier PY (2015) Understanding the effect of cell disruption methods on the diffusion of Chlorella vulgaris proteins and pigments in the aqueous phase. Algal Res 8:61–68

    Google Scholar 

  • Safi C, Rodriguez C, Mulder WJ, Engelen-Smit N, Spekking W, Van den Broek LAM, Olivieri G, Sijtsma L (2017) Energy consumption and water-soluble protein release by cell wall disruption of Nannochloropsis gaditana. Bioresour Technol 239:204–210

    CAS  PubMed  Google Scholar 

  • Samarasinghe N, Fernando S, Lacey R, Faulkner WB (2012) Algal cell rupture using high pressure homogenization as a prelude to oil extraction. Renew Energy 48:300–308

    CAS  Google Scholar 

  • Shene C, Monsalve MT, Vergara D, Lienqueo ME, Rubilar M (2016) High pressure homogenization of Nannochloropsis oculta for the extraction of intracellular components: effect of process conditions and culture age. Eur J Lipid Sci Technol 118:631–639

    CAS  Google Scholar 

  • Silva ADSE, de Magalhãesa WT, Moreiraa LM, Rochab MVP, Bastosa AKP (2018) Microwave-assisted extraction of polysaccharides from Arthrospira (Spirulina) platensis using the concept of green chemistry. Algal Res 35:178–184

    Google Scholar 

  • Soto-Sierra L, Stoykova P, Nikolov ZL (2018) Extraction and fractionation of microalgae-based protein products. Algal Res 36:175–192

    Google Scholar 

  • Spiden EM, Yap BHJ, Hill DRA, Kentish SE, Scales PJ, Martin GJO (2013) Quantitative evaluation of the ease of rupture of industrially promising microalgae by high pressure homogenization. Bioresour Technol 140:165–171

    CAS  PubMed  Google Scholar 

  • Suarez Garcia E, Van Leeuwen J, Safi C, Sijtsma L, Eppink MHM, Wijffels RH, Van den Berg C (2018) Selective and energy efficient extraction of functional proteins from microalgae for food applications. Bioresour Technol 268:197–203

    CAS  PubMed  Google Scholar 

  • Trujillo AJ, Roig-Sagués AX, Zamora A, Ferragut V (2016) High-pressure homogenization for structure modification. In: Knoerzer K, Juliano P, Smithers J (eds) Innovative food processing technologies: extraction. Woodhead Publishing, Amsterdam, pp 315–344

    Google Scholar 

  • Vernès L, Abert-Vian M, El Maâtaoui M, Tao Y, Bornard I, Chemat F (2019) Application of ultrasound for green extraction of proteins from spirulina. Mechanism, optimization, modeling, and industrial prospects. Ultrason Sonochem 54:48–60

    PubMed  Google Scholar 

  • Wu X, Li R, Zhao Y, Liu Y (2017) Separation of polysaccharides from Spirulina platensis by HSCCC with ethanol-ammonium sulfate ATPS and their antioxidant activities. Carbohydr Polym 173:465–472

    CAS  PubMed  Google Scholar 

  • Xie Y, Ho SH, Chen CN, Chen CY, Jing K, Ng IS, Chen J, Chang JS, Lu Y (2016) Disruption of thermo-tolerant Desmodesmus sp. F51 in high pressure homogenization as a prelude to carotenoids extraction. Biochem Eng J 109:243–251

    CAS  Google Scholar 

  • Yap BH, Dumsday GJ, Scales PJ, Martin GJ (2014) Energy evaluation of algal cell disruption by high pressure homogenisation. Bioresour Technol 184:280–285

    PubMed  Google Scholar 

  • Zhang Z, Wang F, Wang X, Liu X, Hou Y, Zhang Q (2010) Extraction of the polysaccharides from five algae and their potential antioxidant activity in vitro. Carbohydr Polym 82:118–121

    CAS  Google Scholar 

Download references

Acknowledgments

The authors wish to acknowledge CBB Capbiotek for research program coordination and the CNRS (Marine biologic station of Roscoff, France) for HPSEC analysis.

Funding

This work was supported by the Regional Council of Brittany in the framework of the “Transfert de technologies des filières « Santé » et « Biotechnologies »” program (grant number 17001728).

Author information

Authors and Affiliations

  1. IRDL - UMR CNRS 6027, Université Bretagne-Sud, 56300, Pontivy, France

    A. Elain, C. Nkounkou & M. Le Fellic

  2. PFT ProDiaBio, Allée des Pommiers, 56300, Pontivy, France

    K. Donnart

Authors
  1. A. Elain
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Nkounkou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Le Fellic
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Donnart
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. Elain.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Elain, A., Nkounkou, C., Le Fellic, M. et al. Green extraction of polysaccharides from Arthrospira platensis using high pressure homogenization. J Appl Phycol 32, 1719–1727 (2020). https://doi.org/10.1007/s10811-020-02127-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10811-020-02127-y

Keywords

Search

Navigation