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Effect of epiphyte infection on physical and chemical properties of carrageenan produced by Kappaphycus alvarezii Doty (Soliericeae, Gigartinales, Rhodophyta)

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Abstract

Epiphytism of filamentous red algae is a serious problem in Kappaphycus farms in the Philippines, Indonesia, Malaysia, and Tanzania. The causative organism of epiphyte outbreak has been identified as Neosiphonia apiculata (Hollenberg) Masuda and Kogame, but its actual effect on carrageenan quality has not yet been established. Therefore, yield and quality of carrageenan from healthy and infected specimens were examined. Infected specimens showed 20.5 ± 2.5 % DW lower carrageenan yield compared with the healthy seaweed (65.5 ± 4.2 % DW). Infected specimens also had a higher phenolic and fatty acid content, compared with healthy specimens. The carrageenan from the infected seaweed showed 74.5 ± 2.8 % lower viscosity, 52.6 ± 3.6 % lower gel strength, 22.9 ± 1.5 % higher syneresis, and 5 °C higher melting temperature as compared with carrageenan from healthy specimens. FTIR and 13C-NMR analysis of carrageenan from infected seaweed did not show any differences in their functionality or carbon atom chemical shift as compared with healthy and standard k-carrageenan. However, size exclusion chromatography showed the infected carrageenan molecular size to be 80 kDa as compared with 800 kDa for the healthy and standard k-carrageenan. These findings prove that infection of Kappaphycus by the filamentous red algae epiphyte, N. apiculata, reduces carrageenan molecular size and affects the physical properties of the carrageenan.

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Acknowledgments

The authors would like to acknowledge the PEMANDU for funding this project. In addition, CSV would like to thank the Japan Society for the Promotion of Science (JSPS) and Sabah Parks Semporna for the permission to conduct surveys and collect seaweed materials form culture farms in Semporna, Sabah.

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Authors and Affiliations

  1. Laboratory of Natural Products Chemistry, Institute for Tropical Biology and Conservation, University Malaysia Sabah, 88999, Kota Kinabalu, Sabah, Malaysia

    Charles S. Vairappan & Chong Sim Chung

  2. Laboratory of Aquatic Natural Products Chemistry, Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-8657, Japan

    Shigeki Matsunaga

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  1. Charles S. Vairappan
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  2. Chong Sim Chung
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  3. Shigeki Matsunaga
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Correspondence to Charles S. Vairappan.

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Vairappan, C.S., Chung, C.S. & Matsunaga, S. Effect of epiphyte infection on physical and chemical properties of carrageenan produced by Kappaphycus alvarezii Doty (Soliericeae, Gigartinales, Rhodophyta). J Appl Phycol 26, 923–931 (2014). https://doi.org/10.1007/s10811-013-0126-0

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  • DOI: https://doi.org/10.1007/s10811-013-0126-0

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